Detailed information NTG0239314

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: Asalp_RS20640
• Genome accession: NZ_CP022426
• Coordinates: 4410929..4412611 (+)
• Length: 560 a.a.
• NCBI ID: WP_034524152.1
• Uniprot ID: -
• Organism: Aeromonas salmonicida subsp. pectinolytica 34mel
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	4409861..4410892	4410929..4412611	flank	37

Gene organization within MGE regions

Location: 4409861..4412611

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Asalp_RS20635 (Asalp_40400)	-	4409861..4410892 (+)	1032	WP_001809438.1	IS630-like element ISAhy2 family transposase	-
Asalp_RS20640 (Asalp_40390)	pilB	4410929..4412611 (+)	1683	WP_034524152.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene

Sequence

Protein

• Length: 560 a.a.
• Molecular weight: 61788.23 Da
• Isoelectric Point: 5.4384

>NTDB_id=239314 Asalp_RS20640 WP_034524152.1 4410929..4412611(+) (pilB) [Aeromonas salmonicida subsp. pectinolytica 34mel]
MALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKYLNQKLIEK
HHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEISELEVSDE
NSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSARLKVMARLD
IAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQGMILVTGP
TGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAI
KAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFTQQQLAAGL
RLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLLIAAIAQKEGMRTLRVSGLEKARLGVTSLAEINRVTTN

Nucleotide

• Length: 1683 bp

>NTDB_id=239314 Asalp_RS20640 WP_034524152.1 4410929..4412611(+) (pilB) [Aeromonas salmonicida subsp. pectinolytica 34mel]
CTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTACCTGAGCCAAGCCAAGGCACAGCG
CAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGGCGGATTTTTGCGAGCTGGAATATG
GGGTACCTCTGCTGGATCTTGCCGCTTTCGACCTGGCCGAGATCCCGCAGAAGTACCTCAATCAGAAGCTGATTGAGAAA
CACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGACCCGACCAATGTGTCGGCACTGGA
GGATTTTGGCTTCAGCTTCGGACTCCATACCGAAGCCCTGCTGGTGGAAGAGAACAAGCTCACCGCCGCCATCGGCAAGC
TGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCTGAGATCTCCGAGCTGGAAGTCTCGGACGAA
AACTCCCGCCTCGATGAGAGCGTCAATACCGCAGATGACGATGCCCCCATCGTCAAATACATCAATAAAATCATGATGGA
TGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACCGTATCCGCTTTCGTATAGACGGCA
TACTGCACGAGATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGCCTCAAGGTGATGGCGCGACTCGAT
ATCGCCGAGCGACGACTGCCCCAGGACGGTCGCATCAAGCTCAAGCTGTCGCGCAACAAATCCATGGATATGCGGGTCAA
CACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCGCAGCGCGGCTCAATATCGAACAGC
TCGGCTTCGACGACCGGCAGAAGGCACAATATCTGCGTGCCCTCTCCAAGCCCCAGGGCATGATACTGGTGACGGGCCCG
ACCGGCTCTGGCAAGACTGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCACAGAGGTCAACATCTCTACCGCCGA
AGATCCGGTGGAGATCAACCTGCCCGGTGTCAACCAGGTACAAGTCAATCCCAAGGCGGGGCTGACCTTCGCCAGCGCCC
TGCGCTCTTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGATCTGGAAACCGCCGAGATTGCCATC
AAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGCCGAAACCCTGACCCGGATGATGAA
CATGGGGGTCCCGGCCTTCAATATCGCCTCTTCAGTAACCCTCATCATGGCCCAGCGCCTCGCTCGCAAGCTCTGCGATC
ACTGCAAGGCGCCGGAAGTGGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACCCAGCAGCAGTTGGCCGCCGGCTTG
CGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGTCGGTATCTACGAAATCATGCTAAT
GTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCTAATTGCAGCGATTGCTCAAAAAGAGGGGATGC
GAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCAGAGATCAACCGGGTGACCACCAAC
TGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (34-139)

• Type II/IV secretion system protein (T2SSE) (183-450)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	56.194	99.464	0.559
pilB	Acinetobacter baumannii D1279779	56.545	98.214	0.555
pilB	Legionella pneumophila strain ERS1305867	54.902	100	0.55
pilB	Vibrio cholerae strain A1552	54.336	100	0.548
pilB	Vibrio campbellii strain DS40M4	52.5	100	0.525
pilB	Vibrio parahaemolyticus RIMD 2210633	51.885	99.464	0.516
pilF	Neisseria gonorrhoeae MS11	46.512	99.821	0.464
pilB/pilB1	Synechocystis sp. PCC 6803	38.879	98.75	0.384
pilF	Thermus thermophilus HB27	40.341	94.286	0.38
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.149	86.429	0.364