Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CCX46_RS02085 Genome accession   NZ_CP022411
Coordinates   458311..460383 (+) Length   690 a.a.
NCBI ID   WP_127925530.1    Uniprot ID   -
Organism   Pseudomonas sp. RU47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453311..465383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX46_RS02065 (CCX46_02035) pilM 455523..456587 (+) 1065 WP_127925526.1 pilus assembly protein PilM Machinery gene
  CCX46_RS02070 (CCX46_02040) - 456587..457153 (+) 567 WP_127925527.1 PilN domain-containing protein -
  CCX46_RS02075 (CCX46_02045) pilO 457150..457773 (+) 624 WP_127925528.1 type 4a pilus biogenesis protein PilO -
  CCX46_RS02080 (CCX46_02050) - 457770..458297 (+) 528 WP_127925529.1 pilus assembly protein PilP -
  CCX46_RS02085 (CCX46_02055) pilQ 458311..460383 (+) 2073 WP_127925530.1 type IV pilus secretin PilQ Machinery gene
  CCX46_RS02090 (CCX46_02060) aroK 460388..460906 (+) 519 WP_045122915.1 shikimate kinase AroK -
  CCX46_RS02095 (CCX46_02065) aroB 461059..462159 (+) 1101 WP_127925531.1 3-dehydroquinate synthase -
  CCX46_RS02100 (CCX46_02070) - 462170..463762 (+) 1593 WP_127925532.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74758.28 Da        Isoelectric Point: 5.8240

>NTDB_id=239221 CCX46_RS02085 WP_127925530.1 458311..460383(+) (pilQ) [Pseudomonas sp. RU47]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYTTRVAGNNLFVVVGQGAPAAAPRSAVVAPRPAAAAPAKAVVPKSRAIRGV
DFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFSRTQLPEKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSGTY
EYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKI
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVNGS
STTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=239221 CCX46_RS02085 WP_127925530.1 458311..460383(+) (pilQ) [Pseudomonas sp. RU47]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGCTGTCGTTCGACGGCCCACCGCCACAGCCCAAGG
GCTACACCACCGAATCACCGGCACGCATCGCCCTCGATTTGCCCGGTGTCGCCAGTCAATTGGCCAGCAAGAACCTTGAT
CTGGGCAGCGGCAATGCACGCACCGCTACGGTGGTCGAGGCCAAGGATCGCACGCGGCTGATCGTCAGTCTCACGCAACT
GGCGCCTTACACCACGCGGGTCGCGGGCAACAATCTGTTCGTGGTGGTTGGGCAGGGCGCGCCGGCGGCAGCGCCGCGAT
CTGCTGTCGTTGCGCCACGCCCTGCGGCTGCAGCGCCGGCCAAGGCAGTTGTGCCGAAAAGCCGGGCTATTCGCGGCGTA
GATTTCCAGCGGGGTACGGCAGGTGAAGGCAACGTGGTCATTGACCTCTCCGACCCGACCATCGCTCCGGATATCCAGGA
GCATGACGGCAAGATCATCCTCAGCTTTTCCCGCACACAACTCCCGGAAAAGCTGCGGGTACGCCTCGACGTCAAGGATT
TCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTGACCGCTGATCGCACGGTTATTACGGTTGAACCCAGTGGTACTTAC
GAGTATTCGACTTTCCAGACCGACAACAAACTGACCGTCAGCATCCGCCCGATGACCGTCGATGATCTGCAAAAGCGTAA
CGCCGATCGTCAGGCTTACGTCGGCGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCAGTGCTGCAACTGA
TCGCCGATTTCACCAACCTCAACCTTGTCGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGCCTGCAGAACGTGCCG
TGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAATGTGCTGCTGGTTGCCCC
GGCCGATGAAATCGCTGCCCGCGAACGTCAGGAACTGGAGTCGCAGAAACAGATCGCCGAACTGGCACCGCTGCGTCGCG
AACTGTTGCAAGTGAACTACGCGAAAGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCTAAAATC
GACGAGCGCGGTTCGATTACTGTCGATGAGCGGACCAACAACATCATTGCCTATCAGACTCAGGATCGTCTCGACGAACT
GCGGCGGATCGTGGCACAGCTGGATATACCGGTACGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCTAACGTCGATT
ACGACAAAAGCTTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAACGGATCG
TCGACCACCATTGGTACTCCCGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACCGTCAACAATACTTCAGG
AATCGGCATCGCTTTCATCACCGACAACGTCTTGCTCGATCTTGAACTGACGGCCATGGAGAAAACCGGCAACGGCGAAA
TCGTCTCGCAGCCGAAAGTGGTCACCTCCGACAAGGAGACCGCGAAAATCCTCAAAGGCACCGAGATTCCGTATCAGGAA
GCCAGCTCCAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACGCCGCAGATCACCCCCGA
CAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGCCAATCA
AGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGGGGCGTTTTCTCAAATACTCAA
AGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.082

100

0.759
  comQ Acinetobacter baylyi ADP1

41.474

100

0.416
  pilQ Acinetobacter baumannii strain A118

38.566

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.566

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.314

97.971

0.375
  pilQ Legionella pneumophila strain ERS1305867

38.279

97.681

0.374

Multiple sequence alignment