Detailed information NTG0238464

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CFN60_RS12455
• Genome accession: NZ_CP022341
• Coordinates: 2551157..2551771 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain 157
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2546157..2556771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFN60_RS12430 (CFN60_12425)	rpsT	2546460..2546726 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CFN60_RS12435 (CFN60_12430)	holA	2546743..2547784 (-)	1042	Protein_2381	DNA polymerase III subunit delta	-
CFN60_RS19990	-	2547824..2547975 (-)	152	Protein_2382	hypothetical protein	-
CFN60_RS12440 (CFN60_12435)	-	2548016..2548150 (+)	135	WP_003152870.1	YqzM family protein	-
CFN60_RS12445 (CFN60_12440)	comEC	2548169..2550520 (-)	2352	WP_089072447.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CFN60_RS12450 (CFN60_12445)	-	2550521..2551090 (-)	570	WP_003152868.1	ComE operon protein 2	-
CFN60_RS12455 (CFN60_12450)	comEA	2551157..2551771 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CFN60_RS12460 (CFN60_12455)	comER	2551830..2552651 (+)	822	WP_014418420.1	late competence protein ComER	-
CFN60_RS12465 (CFN60_12460)	-	2552720..2553457 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
CFN60_RS12470 (CFN60_12465)	rsfS	2553454..2553810 (-)	357	WP_007408260.1	ribosome silencing factor	-
CFN60_RS12475 (CFN60_12470)	yqeK	2553828..2554388 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CFN60_RS12480 (CFN60_12475)	-	2554378..2554947 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CFN60_RS12485 (CFN60_12480)	yhbY	2554958..2555248 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CFN60_RS12490 (CFN60_12485)	aroE	2555242..2556084 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=238464 CFN60_RS12455 WP_014418419.1 2551157..2551771(-) (comEA) [Bacillus velezensis strain 157]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=238464 CFN60_RS12455 WP_014418419.1 2551157..2551771(-) (comEA) [Bacillus velezensis strain 157]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363