Detailed information NTG0238462

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: CFN60_RS12445
• Genome accession: NZ_CP022341
• Coordinates: 2548169..2550520 (-)
• Length: 783 a.a.
• NCBI ID: WP_089072447.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 157
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2543169..2555520

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFN60_RS12415 (CFN60_12410)	-	2543535..2543873 (-)	339	WP_007408270.1	YqxA family protein	-
CFN60_RS12420 (CFN60_12415)	spoIIP	2543890..2545083 (-)	1194	WP_007612673.1	stage II sporulation protein P	-
CFN60_RS12425 (CFN60_12420)	gpr	2545151..2546257 (-)	1107	WP_014418417.1	GPR endopeptidase	-
CFN60_RS12430 (CFN60_12425)	rpsT	2546460..2546726 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CFN60_RS12435 (CFN60_12430)	holA	2546743..2547784 (-)	1042	Protein_2381	DNA polymerase III subunit delta	-
CFN60_RS19990	-	2547824..2547975 (-)	152	Protein_2382	hypothetical protein	-
CFN60_RS12440 (CFN60_12435)	-	2548016..2548150 (+)	135	WP_003152870.1	YqzM family protein	-
CFN60_RS12445 (CFN60_12440)	comEC	2548169..2550520 (-)	2352	WP_089072447.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CFN60_RS12450 (CFN60_12445)	-	2550521..2551090 (-)	570	WP_003152868.1	ComE operon protein 2	-
CFN60_RS12455 (CFN60_12450)	comEA	2551157..2551771 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CFN60_RS12460 (CFN60_12455)	comER	2551830..2552651 (+)	822	WP_014418420.1	late competence protein ComER	-
CFN60_RS12465 (CFN60_12460)	-	2552720..2553457 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
CFN60_RS12470 (CFN60_12465)	rsfS	2553454..2553810 (-)	357	WP_007408260.1	ribosome silencing factor	-
CFN60_RS12475 (CFN60_12470)	yqeK	2553828..2554388 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CFN60_RS12480 (CFN60_12475)	-	2554378..2554947 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CFN60_RS12485 (CFN60_12480)	yhbY	2554958..2555248 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86814.71 Da
• Isoelectric Point: 9.0097

>NTDB_id=238462 CFN60_RS12445 WP_089072447.1 2548169..2550520(-) (comEC) [Bacillus velezensis strain 157]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLFFIIVKTKQHAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWTASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTVFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSV
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLSGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEEIRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=238462 CFN60_RS12445 WP_089072447.1 2548169..2550520(-) (comEC) [Bacillus velezensis strain 157]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTTTCTTTATTATTGTAAAAACAAAGCAGCATGCTCCGGTTATTGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAACATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GACGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGTTCTCTGGAGCAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCCGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGGTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACTCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGTT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAAGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAATCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (208-469)

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Domain of unknown function (DUF4131) (DUF4131) (26-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.792	98.723	0.561