Detailed information NTG0238197

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CFH90_RS07725
• Genome accession: NZ_CP022298
• Coordinates: 1563150..1564343 (+)
• Length: 397 a.a.
• NCBI ID: WP_126036367.1
• Uniprot ID: A0A3S9AJX4
• Organism: Acinetobacter johnsonii strain IC001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1558150..1569343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFH90_RS07715 (CFH90_07695)	-	1559266..1561365 (+)	2100	WP_126036365.1	EAL domain-containing protein	-
CFH90_RS18960	-	1561758..1561904 (-)	147	WP_171254088.1	hypothetical protein	-
CFH90_RS07720 (CFH90_07700)	-	1561850..1562911 (+)	1062	WP_004693246.1	3-deoxy-7-phosphoheptulonate synthase	-
CFH90_RS07725 (CFH90_07705)	pilU	1563150..1564343 (+)	1194	WP_126036367.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CFH90_RS07730 (CFH90_07710)	-	1564390..1565568 (-)	1179	WP_126036368.1	MFS transporter	-
CFH90_RS07735 (CFH90_07715)	gcvH	1565828..1566202 (-)	375	WP_004693240.1	glycine cleavage system protein GcvH	-
CFH90_RS07740 (CFH90_07720)	-	1566274..1567302 (-)	1029	WP_126036370.1	AraC family transcriptional regulator	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44766.18 Da
• Isoelectric Point: 6.3912

>NTDB_id=238197 CFH90_RS07725 WP_126036367.1 1563150..1564343(+) (pilU) [Acinetobacter johnsonii strain IC001]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPCDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDIIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRATVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLNSSSALSFDQAVAEKAKEAEEEKDDA

Nucleotide

• Length: 1194 bp

>NTDB_id=238197 CFH90_RS07725 WP_126036367.1 1563150..1564343(+) (pilU) [Acinetobacter johnsonii strain IC001]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTCATGAAGCCACTGGGTCAACAAAACTTGC
CATGTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACTGAGCTGGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTTTCACGTTTTCGTGTCAATGTTTTTCAGCAGCAACTGCATGTAGGTATGGTGAT
TCGTACGATTACTGCGGAAATTCCTAACTTCACTAAGTTACAACTTCCAACGTCTTTAAAAGACATAATTATGGAAAAAC
GTGGTTTGGTCCTCGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTAGCAGCCATGATTGATCATCGAAATGAA
AACTCAGCAGGGCACATTATTACGGTTGAAGATCCTGTGGAGTATGTACATAAACATAAGAAATCGATGATTACTCATCG
AGAAGTGGGTGTGGACTGTCATTCTTGGCACAATGCTTTGAAAAATACTCTGCGCCAAGCACCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACTATGGAGCATGCCATAGCTTTTGCGGAAACAGGTCATTTATGCCTTGGAACATTGCAT
GCCAATAATGCAAATCAAGCTTTAGATCGAATTATTAACTTTTTCCCAGATGAGCGTCGCAATCAACTTTTGATGGACTT
ATCCTCCAATATGAAGGCGATTATTTCACAGCGTTTGGTGCGAACTGAAGATGGGCGTGGTCGCCGAGCAACAGTAGAAA
TCATGCTTAATACACCCTTGATGTCAGACTTGATACTTAAAGGGAATTTCCACGAACTCAAAGAAGTGATGTCGAAGTCA
CGAGAGCTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATTGCTTACGAAGAAGCGCT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCTAACCCACAGTTAAACTCAA
GTAGTGCTTTGTCTTTTGATCAAGCGGTTGCCGAAAAGGCCAAAGAAGCTGAAGAAGAGAAAGACGACGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-282)

3D structure

• AlphaFold DB: A0A3S9AJX4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.331	90.428	0.537
pilU	Acinetobacter baylyi ADP1	54.545	91.436	0.499
pilU	Vibrio cholerae strain A1552	50.578	87.154	0.441
pilT	Acinetobacter baylyi ADP1	43.598	82.62	0.36
pilT	Legionella pneumophila strain Lp02	42.183	85.39	0.36
pilT	Legionella pneumophila strain ERS1305867	42.183	85.39	0.36