Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFI10_RS13890 Genome accession   NZ_CP022297
Coordinates   2896139..2897836 (+) Length   565 a.a.
NCBI ID   WP_206842166.1    Uniprot ID   -
Organism   Marinobacterium iners strain IC961     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2882121..2895865 2896139..2897836 flank 274


Gene organization within MGE regions


Location: 2882121..2897836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFI10_RS13810 (CFI10_13745) - 2882121..2882495 (-) 375 WP_206835406.1 translation initiation factor Sui1 -
  CFI10_RS13815 (CFI10_13750) - 2882561..2883880 (-) 1320 WP_206842156.1 deoxyguanosinetriphosphate triphosphohydrolase -
  CFI10_RS13820 (CFI10_13755) lnt 2883910..2885361 (-) 1452 WP_206835408.1 apolipoprotein N-acyltransferase -
  CFI10_RS13825 (CFI10_13760) - 2885364..2885765 (-) 402 WP_277987729.1 type IV pilin protein -
  CFI10_RS13830 (CFI10_13765) - 2885770..2888700 (-) 2931 WP_206835410.1 PilC/PilY family type IV pilus protein -
  CFI10_RS13835 (CFI10_13770) - 2888650..2890092 (+) 1443 WP_206834310.1 IS66 family transposase -
  CFI10_RS13840 (CFI10_0050) - 2890111..2890770 (-) 660 WP_206835412.1 hypothetical protein -
  CFI10_RS13845 (CFI10_13775) - 2890781..2891422 (-) 642 WP_206835416.1 PilX N-terminal domain-containing pilus assembly protein -
  CFI10_RS13850 (CFI10_13780) - 2891419..2892450 (-) 1032 WP_206835419.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CFI10_RS13855 (CFI10_13785) pilV 2892447..2892923 (-) 477 WP_206835423.1 type IV pilus modification protein PilV -
  CFI10_RS13860 (CFI10_13790) - 2892920..2893405 (-) 486 WP_206842161.1 GspH/FimT family pseudopilin -
  CFI10_RS13865 (CFI10_13795) - 2893691..2893945 (-) 255 WP_206835426.1 hypothetical protein -
  CFI10_RS13870 (CFI10_13800) - 2894023..2894394 (+) 372 Protein_2724 transposase -
  CFI10_RS13875 (CFI10_13805) - 2894396..2894877 (+) 482 Protein_2725 IS3 family transposase -
  CFI10_RS19405 (CFI10_13810) - 2895015..2895389 (-) 375 WP_341868535.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CFI10_RS13885 - 2895746..2895865 (-) 120 Protein_2727 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CFI10_RS13890 (CFI10_13820) pilB 2896139..2897836 (+) 1698 WP_206842166.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62578.75 Da        Isoelectric Point: 5.1006

>NTDB_id=238165 CFI10_RS13890 WP_206842166.1 2896139..2897836(+) (pilB) [Marinobacterium iners strain IC961]
MQPLTGLAKRLVNDGVFSAEVAQDAIQEAQKAGQPFITYIIKHRLVSAQRAATAASDEFGLPLFDIDAISDELLPKEVVA
EGLITKHHVLPLMRRENRLFIAVSDPTNIQALDEIKFQTGITTEAVIVEDDKLTRRLDQYLEHSSTAMDELEDEDLENLQ
LHPTEQTEEDVAEDAANETPIVRFINRVLLDAIKKGASDIHFEPYEKAYRVRCRIDGILQEVHRPPLNLASRLTARLKVM
SKMDISEKRVPQDGRIKLKVSAKRAIDFRVNTLPTLWGEKIVLRILDPTSAQMGVEALGFEPDQKEAFMSTLEQPQGMIL
VTGPTGSGKTVTLYTGLNILNTEERNISTAEDPVEINLEGVNQVHVNPKVGLTFAEALRAFLRQDPDVVMVGEIRDLETA
EIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCSDCKKPVNLPEATLIEEGFSVEAI
PALHIYEANHKGCNSCNRGYKGRVGIYEILQMTADVAECIMDNGTSIDILRVAKQEGFKTLREAGLIKVANGLTSLEEMN
RVIKT

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=238165 CFI10_RS13890 WP_206842166.1 2896139..2897836(+) (pilB) [Marinobacterium iners strain IC961]
ATGCAACCCCTTACAGGCTTGGCCAAACGCTTGGTCAATGATGGTGTTTTCTCAGCCGAAGTCGCACAGGATGCGATTCA
AGAAGCCCAAAAGGCTGGCCAGCCGTTCATAACCTACATAATCAAGCACAGACTCGTCAGCGCCCAGCGGGCGGCTACTG
CCGCATCTGATGAGTTCGGCTTACCTCTTTTTGACATCGACGCCATCTCTGATGAGCTACTGCCCAAAGAGGTTGTAGCC
GAGGGCCTGATCACCAAGCACCATGTTCTTCCGCTGATGCGTCGGGAAAACAGGTTATTCATTGCCGTTTCCGACCCTAC
AAATATCCAGGCGCTCGACGAAATCAAATTTCAGACTGGCATCACAACTGAGGCTGTCATTGTCGAAGATGACAAGCTAA
CCCGTCGGCTTGACCAGTACCTGGAGCACTCATCAACAGCCATGGACGAGCTGGAAGATGAAGACCTGGAAAACCTGCAA
CTGCATCCAACTGAACAAACGGAAGAAGATGTCGCTGAAGATGCAGCTAACGAAACCCCAATTGTTCGATTTATCAACAG
AGTACTACTGGACGCGATAAAGAAAGGCGCTTCAGATATTCACTTTGAACCGTACGAGAAAGCATACAGGGTACGTTGTC
GTATTGATGGGATACTGCAGGAGGTACACCGCCCTCCCCTGAACCTCGCCAGCCGATTAACTGCTCGCTTGAAGGTCATG
TCCAAGATGGATATATCAGAGAAGCGCGTACCTCAGGACGGACGCATTAAGTTAAAGGTTTCCGCAAAGCGCGCTATTGA
CTTCCGTGTCAACACACTCCCTACTCTTTGGGGTGAAAAGATTGTATTGCGTATTCTCGACCCGACCAGCGCCCAAATGG
GTGTTGAAGCTTTGGGCTTTGAGCCGGATCAGAAAGAAGCCTTCATGTCTACGCTTGAGCAGCCGCAGGGTATGATTTTA
GTCACAGGCCCCACTGGCTCGGGCAAAACAGTAACGCTTTATACCGGTCTCAATATTTTAAATACAGAAGAGCGCAATAT
TTCGACTGCAGAAGACCCGGTAGAGATCAACCTAGAGGGGGTTAACCAGGTCCATGTTAACCCTAAAGTCGGGCTTACAT
TTGCTGAAGCACTTCGTGCCTTTTTGCGCCAAGACCCCGACGTGGTGATGGTGGGTGAGATTCGTGACTTGGAAACAGCC
GAGATTGCGATCAAGGCTGCTCAAACAGGGCACATGGTGCTGTCTACATTGCATACAAATAGTGCACCCGAAACTCTGAC
ACGGTTACGAAACATGGGAGTACCTGCATTCAATATTGCAACATCTGTAAGCTTGATCATAGCTCAGAGACTGGGGCGTC
GACTATGTTCAGACTGTAAAAAACCTGTCAACCTACCAGAGGCCACCTTGATCGAGGAAGGTTTTTCTGTAGAGGCAATA
CCTGCATTGCACATATATGAAGCAAACCATAAAGGTTGTAACAGCTGCAATCGTGGCTATAAGGGACGTGTGGGTATTTA
CGAGATACTGCAGATGACAGCAGACGTGGCTGAGTGCATTATGGATAACGGCACATCTATTGATATACTCAGAGTGGCAA
AACAAGAAGGCTTCAAGACGCTTCGCGAGGCAGGTCTCATCAAAGTTGCCAATGGCTTGACGAGTTTGGAAGAGATGAAC
CGCGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.111

99.292

0.577
  pilB Acinetobacter baylyi ADP1

57.398

99.292

0.57
  pilB Legionella pneumophila strain ERS1305867

55.075

94.159

0.519
  pilB Vibrio cholerae strain A1552

51.59

100

0.517
  pilB Vibrio parahaemolyticus RIMD 2210633

48.85

100

0.489
  pilF Neisseria gonorrhoeae MS11

49.283

98.761

0.487
  pilB Vibrio campbellii strain DS40M4

48.05

99.823

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

35.831

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.345

0.375
  pilF Thermus thermophilus HB27

36.786

99.115

0.365

Multiple sequence alignment