Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BG910_RS07485 Genome accession   NZ_CP022278
Coordinates   1517177..1518856 (+) Length   559 a.a.
NCBI ID   WP_089036302.1    Uniprot ID   A0A220S296
Organism   Neisseria chenwenguii strain 10023     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1512177..1523856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG910_RS07460 (BG910_07450) - 1513434..1513967 (+) 534 WP_089036297.1 septation protein A -
  BG910_RS07465 (BG910_07455) - 1513970..1514260 (+) 291 WP_089036298.1 YciI family protein -
  BG910_RS07470 (BG910_07460) - 1514260..1514529 (+) 270 WP_089036299.1 BolA family transcriptional regulator -
  BG910_RS07475 (BG910_07465) - 1514551..1515423 (+) 873 WP_089036300.1 peptidylprolyl isomerase -
  BG910_RS07480 (BG910_07470) rfaQ 1515595..1516665 (-) 1071 WP_089036301.1 putative lipopolysaccharide heptosyltransferase III -
  BG910_RS07485 (BG910_07475) pilF 1517177..1518856 (+) 1680 WP_089036302.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BG910_RS07490 (BG910_07480) pilG 1518927..1520168 (+) 1242 WP_089036303.1 type II secretion system F family protein Machinery gene
  BG910_RS07495 (BG910_07485) pilD 1520170..1521033 (+) 864 WP_089036304.1 A24 family peptidase Machinery gene
  BG910_RS07500 (BG910_07490) coaE 1521035..1521649 (+) 615 WP_089036305.1 dephospho-CoA kinase -
  BG910_RS07505 (BG910_07495) yacG 1521663..1521848 (+) 186 WP_089036306.1 DNA gyrase inhibitor YacG -
  BG910_RS12565 - 1522133..1523623 (+) 1491 WP_198344769.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62373.85 Da        Isoelectric Point: 6.3577

>NTDB_id=237853 BG910_RS07485 WP_089036302.1 1517177..1518856(+) (pilF) [Neisseria chenwenguii strain 10023]
MSVGLLRVLVQSQIITSQQAEHYNNELKTGKNVLQMLFGDKIITAKALGELLARTFSYPLLDFQYYPRNNILSDILTEEQ
MVQNRCVPIFRRGHKVYLAVSDPTQIQNFQKITFHSGATVDLVVVPDDQLSSLLEWLGQRSTTILKEMNQEQEAAQPAQA
LYIDNEEAEDGPIPRFIHKTLSDALSAGASDIHFEFYEQMARVRFRVDGVLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPSCKKEVERPPVPALKKAGFTDEDLAKDWKL
YRPVGCDSCRGKGFKGRAGIYEVMPITEEMQRVIMNNGTEVDIMNMAYQEGMVDLRRAGLLKVMQGLTSLEEVTANTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=237853 BG910_RS07485 WP_089036302.1 1517177..1518856(+) (pilF) [Neisseria chenwenguii strain 10023]
ATGAGTGTCGGATTATTGCGCGTTTTGGTGCAGAGCCAAATTATTACCAGCCAGCAGGCTGAGCATTACAACAATGAATT
AAAAACGGGTAAAAACGTATTGCAGATGCTGTTTGGCGACAAAATCATCACTGCCAAGGCATTGGGCGAACTGCTTGCCC
GTACGTTCAGCTATCCGTTGCTGGATTTTCAGTATTACCCACGCAACAATATTTTGTCGGACATTCTGACGGAAGAGCAG
ATGGTGCAAAACCGCTGTGTGCCGATTTTCCGCCGTGGTCATAAGGTTTATCTGGCAGTATCCGATCCGACCCAAATCCA
GAATTTCCAGAAAATTACCTTTCATTCGGGCGCGACGGTTGATTTGGTTGTGGTTCCCGACGATCAGCTCAGCTCACTTT
TGGAATGGCTCGGACAGCGTTCGACCACCATTTTGAAAGAAATGAATCAGGAGCAGGAGGCGGCGCAACCGGCCCAAGCG
CTGTATATCGATAATGAAGAGGCGGAAGACGGCCCGATTCCGCGTTTTATCCATAAAACGCTTTCGGATGCTTTGAGCGC
GGGTGCTTCGGATATCCATTTCGAGTTTTACGAACAAATGGCGCGGGTGCGTTTCCGTGTGGACGGCGTGTTGCGCGAAG
TGGTGCAGCCTCCGGTCGCTGTGCGCGGTCAGTTGGCTTCGCGCATCAAGGTGATGGCGCGTTTGGACATTTCGGAAAAA
CGTATTCCGCAAGACGGCCGTATTCAAATCGCCTTCCACAAACACGGCCGGCCGATCGACTTCCGTGTCAGCACTCTGCC
GACTTTGTTCGGCGAAAAAGTGGTGATGCGTATTTTGAACTCGGATGCGGCATCGCTGAATATCGACCAGCTCGGTTTCG
AACCTTTCCAAAAAGAAATGCTGCTGGAGGCGATTCACCGCCCTTACGGCATGGTGTTGGTGACGGGTCCGACCGGTTCG
GGTAAAACGGTTTCGCTTTATACCTGTCTGAACATTCTCAATACGGAAGATGTGAATATTTCCACCGCAGAAGACCCTGC
GGAAATCAACTTGCCGGGGATTAACCAGGTAAACGTCAACGACAAGCAGGGTTTGACCTTTGCCGCCGCGTTGAAATCAT
TCCTGCGCCAAGACCCAGACATCATCATGGTGGGTGAGATTCGCGATTTGGAAACTGCGGATATTGCCATTAAAGCCGCG
CAAACCGGCCACATGGTGTTTTCAACGCTTCATACCAACAATGCGCCAGCAACCCTGTCGCGTATGCTGAATATGGGTGT
TGCGCCGTTTAACATCGCCAGCTCGGTCAGCCTGATTATGGCGCAGCGTCTGCTGCGGCGTTTGTGCCCGAGCTGTAAGA
AAGAGGTGGAGCGTCCGCCTGTACCGGCTTTGAAAAAAGCAGGCTTTACCGACGAAGATTTGGCAAAAGACTGGAAGCTG
TACCGTCCGGTTGGCTGTGACAGCTGCCGCGGAAAAGGCTTCAAAGGCCGTGCCGGTATTTACGAAGTGATGCCGATTAC
CGAAGAAATGCAGCGCGTGATTATGAATAACGGCACCGAAGTCGATATTATGAACATGGCTTATCAGGAGGGTATGGTCG
ATTTGCGTCGCGCCGGTTTGCTCAAAGTTATGCAGGGGCTGACTTCTTTGGAAGAAGTCACCGCAAACACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220S296

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

79.606

100

0.796
  pilB Acinetobacter baylyi ADP1

50.712

100

0.51
  pilB Acinetobacter baumannii D1279779

50.178

100

0.504
  pilB Legionella pneumophila strain ERS1305867

49.907

96.064

0.479
  pilB Vibrio cholerae strain A1552

45.922

100

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

100

0.462
  pilB Vibrio campbellii strain DS40M4

43.794

100

0.442

Multiple sequence alignment