Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFF01_RS16865 Genome accession   NZ_CP022272
Coordinates   3833184..3834890 (+) Length   568 a.a.
NCBI ID   WP_088905557.1    Uniprot ID   A0AAC9U383
Organism   Shewanella marisflavi strain EP1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3828184..3839890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFF01_RS16845 (CFF01_16845) ampD 3828914..3829465 (-) 552 WP_033540800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CFF01_RS16850 (CFF01_16850) nadC 3829743..3830621 (+) 879 WP_088905554.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CFF01_RS16855 (CFF01_16855) - 3830668..3832347 (+) 1680 WP_088905555.1 PglL family O-oligosaccharyltransferase -
  CFF01_RS19225 - 3832773..3832890 (+) 118 Protein_3247 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CFF01_RS16865 (CFF01_16865) pilB 3833184..3834890 (+) 1707 WP_088905557.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFF01_RS16870 (CFF01_16870) pilC 3834979..3836244 (+) 1266 WP_088905558.1 type II secretion system F family protein Machinery gene
  CFF01_RS16875 (CFF01_16875) pilD 3836292..3837203 (+) 912 WP_088905559.1 A24 family peptidase Machinery gene
  CFF01_RS16880 (CFF01_16880) coaE 3837228..3837833 (+) 606 WP_088905560.1 dephospho-CoA kinase -
  CFF01_RS16885 (CFF01_16885) zapD 3837842..3838576 (+) 735 WP_033540806.1 cell division protein ZapD -
  CFF01_RS16890 (CFF01_16890) yacG 3838625..3838837 (+) 213 WP_088905561.1 DNA gyrase inhibitor YacG -
  CFF01_RS16895 (CFF01_16895) mutT 3838838..3839227 (+) 390 WP_088905562.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63061.17 Da        Isoelectric Point: 5.1146

>NTDB_id=237801 CFF01_RS16865 WP_088905557.1 3833184..3834890(+) (pilB) [Shewanella marisflavi strain EP1]
MPSTGLHLGLSTLFIRKGLLSEEQLADAISRSRQNKQSLVSTIVSIKMLSAREVAELCYEEYGTPLLDLSEFDISSIPED
FLNRKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLTIALEKVLEEDLSALDLDGIDEES
LSSIEVTDTDKRQEEQGDASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLAGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRKLYEKMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRQPEEVPEHELLRLGFT
QAQIDEGFTVYKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIANQAKEQGMRDLRDSGLRKVIAGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=237801 CFF01_RS16865 WP_088905557.1 3833184..3834890(+) (pilB) [Shewanella marisflavi strain EP1]
ATGCCATCAACAGGCTTACATCTAGGACTATCAACTTTATTTATTCGTAAAGGTCTCCTTAGCGAAGAACAGTTAGCTGA
TGCAATATCACGGTCACGTCAAAACAAACAATCTCTAGTAAGCACAATAGTTTCAATTAAAATGCTATCAGCTAGAGAGG
TTGCCGAGCTTTGCTATGAAGAATACGGAACACCTCTGCTAGATCTCAGTGAGTTCGATATAAGTAGTATCCCTGAAGAC
TTTCTCAACAGAAAACTCATCGAGAAGCACAGGTGTCTTCCGCTATTCAAAAGAGGCAACCGCCTCTATATTGGCACTTC
AGATCCAACTAACATCGCAGCCTTAGAAGACTTTCAATTCAGCGCCGGTCTGCATGCTGAAGCAATACTCGTTGAAGATG
ATAAACTTACTATTGCCTTAGAGAAAGTCCTCGAAGAAGATCTCTCGGCTCTCGATTTAGATGGTATAGATGAAGAATCA
CTCTCCAGCATTGAGGTTACCGATACAGACAAGCGCCAGGAAGAACAAGGCGATGCTAGTGATGACGCACCTATTGTCAT
CTACATTAATAAGATTCTCACTGACGCTATACGTAAAGGTGCGTCTGATTTACATTTTGAGCCCTATGAGAAACGCTACC
GTATTCGTTTTCGTATCGATGGAATTTTACATGAAGTCTCTGAACCACCGGTGAATTTAGCTGGCCGTATCTCAGCTCGC
CTTAAGGTGATGTCAAAGCTAGATATAGCCGAACGCCGCGTCCCTCAAGATGGCCGCATCAAGATGAAGCTGTCTCGCAC
TAAATCTATCGATTTTCGTGTCAGCACACTGCCCACTATCTGGGGCGAAAAGATCGTAATGCGTATCTTGGACTCCTCTT
CTGCCCAGCTCGGTATCGAAAAGCTCGGCTATGAAGATGACCAACGCAAGCTTTATGAAAAGATGTTGGCAAAGCCTCAA
GGAATGATCTTGGTAACCGGCCCTACAGGCTCGGGGAAAACAGTTTCCCTTTATACAGGCCTTAACATTCTCAATACCGA
AGAGCGCAATATCTCAACCGCTGAAGATCCTGTTGAAATTAACTTAGAAGGCGTCAATCAGGTTCATATTAACTTGAAGG
CAGGACTTACCTTTGCCTCTGCTCTACGCTCATTCCTGCGCCAAGATCCAGATGTGGTCATGGTGGGTGAGATCCGAGAC
TTGGAAACCGCAGAAATTGCAATCAAAGCAGCGCAAACCGGCCACTTAGTGCTATCTACCCTGCATACTAACTCAGCTGC
CGAAACGCTAACTCGTCTGATAAACATGGGTGTGCCTGGTTACAACATTGCCAGCTCTGTTAACCTGATTATTGCCCAGC
GTCTTGCCAGAAGGCTTTGCACCGAATGCCGTCAGCCCGAAGAAGTCCCAGAGCACGAACTACTCAGATTAGGCTTTACT
CAAGCACAAATTGACGAAGGCTTTACCGTATACAAACCTGTTGGCTGTGACCTTTGCTCTGGCGGTTATAAGGGACGAGT
CGGTATCTATGAAGTAATGAAGATGTCAGATGAGATTGCCCGTACTATTATGGAAGGAGGCAACTCGTTACAAATCGCTA
ATCAGGCCAAAGAACAAGGCATGCGAGATCTGAGAGATTCTGGCCTACGCAAGGTCATTGCAGGCGTAACCAGTATCGCC
GAAATAAACCGCGTCACCAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-144)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.467

98.768

0.577
  pilB Acinetobacter baumannii D1279779

57.576

98.768

0.569
  pilB Legionella pneumophila strain ERS1305867

55.773

99.12

0.553
  pilB Vibrio cholerae strain A1552

52.355

97.183

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.973

99.472

0.507
  pilB Vibrio campbellii strain DS40M4

50.446

98.768

0.498
  pilF Neisseria gonorrhoeae MS11

47.434

99.472

0.472
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

98.415

0.382
  pilF Thermus thermophilus HB27

37.895

100

0.38

Multiple sequence alignment