Detailed information NTG0235511

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CEG11_RS12365
• Genome accession: NZ_CP021976
• Coordinates: 2520597..2521211 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain T20E-257
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2515597..2526211

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CEG11_RS12340 (CEG11_12340)	rpsT	2515897..2516163 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CEG11_RS12345 (CEG11_12345)	holA	2516180..2517221 (-)	1042	Protein_2369	DNA polymerase III subunit delta	-
CEG11_RS19605	-	2517261..2517415 (-)	155	Protein_2370	hypothetical protein	-
CEG11_RS12350 (CEG11_12350)	-	2517456..2517590 (+)	135	WP_003152870.1	YqzM family protein	-
CEG11_RS12355 (CEG11_12355)	comEC	2517609..2519960 (-)	2352	WP_003152869.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CEG11_RS12360 (CEG11_12360)	-	2519961..2520530 (-)	570	WP_003152868.1	ComE operon protein 2	-
CEG11_RS12365 (CEG11_12365)	comEA	2520597..2521211 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
CEG11_RS12370 (CEG11_12370)	comER	2521270..2522091 (+)	822	WP_003152866.1	late competence protein ComER	-
CEG11_RS12375 (CEG11_12375)	-	2522160..2522897 (-)	738	WP_003152865.1	class I SAM-dependent DNA methyltransferase	-
CEG11_RS12380 (CEG11_12380)	rsfS	2522894..2523250 (-)	357	WP_003152864.1	ribosome silencing factor	-
CEG11_RS12385 (CEG11_12385)	yqeK	2523247..2523828 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CEG11_RS12390 (CEG11_12390)	-	2523818..2524387 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
CEG11_RS12395 (CEG11_12395)	yhbY	2524398..2524688 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CEG11_RS12400 (CEG11_12400)	aroE	2524682..2525524 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=235511 CEG11_RS12365 WP_003152867.1 2520597..2521211(-) (comEA) [Bacillus velezensis strain T20E-257]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=235511 CEG11_RS12365 WP_003152867.1 2520597..2521211(-) (comEA) [Bacillus velezensis strain T20E-257]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363