Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BJE04_RS01350 Genome accession   NC_005139
Coordinates   227204..228703 (+) Length   499 a.a.
NCBI ID   WP_011149205.1    Uniprot ID   -
Organism   Vibrio vulnificus YJ016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 222204..233703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJE04_RS01330 (VV0208) hslO 222596..223471 (-) 876 WP_011078947.1 Hsp33 family molecular chaperone HslO -
  BJE04_RS01335 (VV0209) hslR 223503..223889 (-) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  BJE04_RS01340 (VV0213) gspC 224274..225149 (+) 876 WP_157401904.1 type II secretion system protein GspC -
  BJE04_RS01345 (VV0214) gspD 225186..227204 (+) 2019 WP_011149204.1 type II secretion system secretin GspD -
  BJE04_RS01350 (VV0215) pilB/pilB1 227204..228703 (+) 1500 WP_011149205.1 type II secretion system ATPase GspE Machinery gene
  BJE04_RS01355 (VV0216) gspF 228703..229920 (+) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  BJE04_RS01360 (VV0217) gspG 229974..230417 (+) 444 WP_017420501.1 type II secretion system major pseudopilin GspG -
  BJE04_RS01365 (VV0218) gspH 230344..231048 (+) 705 WP_011149207.1 type II secretion system minor pseudopilin GspH -
  BJE04_RS01370 (VV0219) gspI 231041..231403 (+) 363 WP_043877023.1 type II secretion system minor pseudopilin GspI -
  BJE04_RS01375 (VV0220) gspJ 231390..232091 (+) 702 WP_011149209.1 type II secretion system minor pseudopilin GspJ -
  BJE04_RS01380 (VV0221) gspK 232084..233106 (+) 1023 WP_011078937.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55860.17 Da        Isoelectric Point: 6.0826

>NTDB_id=23545 BJE04_RS01350 WP_011149205.1 227204..228703(+) (pilB/pilB1) [Vibrio vulnificus YJ016]
MVDILDTAPSYRRLPFSFANRFKMVLEIEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=23545 BJE04_RS01350 WP_011149205.1 227204..228703(+) (pilB/pilB1) [Vibrio vulnificus YJ016]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AATCGAGCACCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGTCAGCTGATGGAGGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGTTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GGGCGTCAGATATTCACATCGAAACGTTCGAGAAAATTCTGTCGATTCGTTTCCGCGTCGATGGTGTTTTGCGCGATGTG
CTGTCTCCGAGCCGCAAACTGGCGCCGCTGTTGGTCTCTCGCGTTAAAGTCATGGCCAAACTGGATATTGCAGAAAAACG
CGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCGGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGAGAGCGTGTAGTGATGCGTTTGCTGGATAAAAACGCCACTCGCCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGCCCTCACGGCATTATCTTGGTCACAGGTCCAACCGGCTCAGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAATATCTTGACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGCATTGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAATACCGCCGTAGGCGCCATTACCCGTCTGCGTGACATGGGCATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGCGTGTTGGCTCAGCGCCTGGTGCGGACCTTATGCCAAGACTGTAAAGAACCGTAC
GAGGCTGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGTGCCAAGGGATGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTCCAAGAGCTGA
TCCATGCTGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCGAGCATTCGCAGCGATGGTTTAGAC
AAAGTGTTGCAAGGCGTGACCTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.51

98.998

0.421
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363

Multiple sequence alignment