Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CCX87_RS03940 Genome accession   NZ_CP021648
Coordinates   821761..823497 (-) Length   578 a.a.
NCBI ID   WP_087743918.1    Uniprot ID   -
Organism   Acidovorax sp. T1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816761..828497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX87_RS03910 - 817441..817875 (+) 435 WP_087743906.1 NUDIX domain-containing protein -
  CCX87_RS03915 - 817966..818184 (-) 219 WP_087743907.1 DNA gyrase inhibitor YacG -
  CCX87_RS03920 zapD 818198..818953 (-) 756 WP_087743909.1 cell division protein ZapD -
  CCX87_RS03925 coaE 819037..819645 (-) 609 WP_198314761.1 dephospho-CoA kinase -
  CCX87_RS03930 - 819648..820520 (-) 873 WP_087743913.1 A24 family peptidase -
  CCX87_RS03935 pilC 820520..821740 (-) 1221 WP_087743915.1 type II secretion system F family protein Machinery gene
  CCX87_RS03940 pilB 821761..823497 (-) 1737 WP_087743918.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCX87_RS03950 - 823745..824674 (-) 930 WP_198314762.1 polyprenyl synthetase family protein -
  CCX87_RS03955 rplU 824905..825216 (+) 312 WP_024814187.1 50S ribosomal protein L21 -
  CCX87_RS03960 rpmA 825232..825489 (+) 258 WP_087743920.1 50S ribosomal protein L27 -
  CCX87_RS03965 obgE 825657..826727 (+) 1071 WP_087743922.1 GTPase ObgE -
  CCX87_RS03970 proB 826821..827963 (+) 1143 WP_087743925.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62872.10 Da        Isoelectric Point: 5.5910

>NTDB_id=232247 CCX87_RS03940 WP_087743918.1 821761..823497(-) (pilB) [Acidovorax sp. T1]
MAAVDTVQKEASSVALPGLGRALISAGKLTQKSAEEIYKKSQTSRTSFIAELTGSGIVSASDLAHTVSAVFGAPLLDLDA
IDPLRLPKDLLDAKICNAYRVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLSRLVENSTKSTSESM
DTLISGGGDFEFDDVTVEEATESTDAGSDVEDAPVVKFVHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEAVEKE
RLLAAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKTPADIP
HETLVDAGFPEHEIDGSWVTYRPVGCSACNNGYKGRLGIYQVMPITEEIQRIILRDGSALEIAEQAKREGVRSLRDAGLH
KAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=232247 CCX87_RS03940 WP_087743918.1 821761..823497(-) (pilB) [Acidovorax sp. T1]
ATGGCCGCTGTTGATACTGTCCAGAAAGAAGCCTCCTCAGTAGCCCTCCCAGGATTGGGCCGCGCGCTGATATCGGCGGG
AAAGCTGACACAAAAATCAGCCGAGGAAATTTACAAAAAGTCACAAACAAGTCGCACCAGCTTTATCGCTGAGTTAACCG
GCTCAGGCATCGTTTCCGCCTCGGATTTGGCGCACACCGTTTCGGCTGTTTTTGGCGCACCATTGCTGGACCTGGACGCC
ATAGATCCACTGCGTCTTCCCAAAGACCTGCTCGACGCCAAAATTTGCAACGCTTACCGGGTGGTTGTTCTCAGCAAAAG
AAACAATCGGCTCATAGTGGCCACGGCAGATCCAACAGATCAGGAAGCCGCCGAGAAAATCAAATTCACCACCCAAATGG
GGGTGGACTGGATTATTGCAGAATACGATAAGTTAAGTCGTCTGGTAGAAAACAGCACAAAATCCACCAGCGAATCCATG
GATACGCTGATCAGTGGCGGGGGTGATTTTGAATTCGATGATGTCACTGTTGAAGAAGCCACGGAGAGTACGGATGCTGG
AAGCGACGTTGAAGACGCTCCAGTCGTCAAGTTTGTGCACAAGATGCTGCTCGATGCTTTCAATATGCGGGCCTCTGATC
TGCATTTCGAACCTTATGAGCACCAGTATCGGGTGCGGTTTCGTATTGATGGAGAATTGCGCGAAATTGCATCCCCCCCT
ATTGCCATCAAGGACAAATTAGCCTCCCGCATCAAGGTTATTTCGCGCCTCGATATTTCCGAGAAACGGGTGCCCCAGGA
TGGGCGCATGAAATTGAAAGTGGGTCCCGACCGGGTCATCGACTTTCGTGTGAGCACCTTGCCCACGCTGTTTGGCGAAA
AAATCGTGATTCGTATTTTGGATCCCAGCAGCGCGAAACTCGGCATTGATGCGCTAGGTTATGAGGCCGTGGAGAAAGAG
CGCCTACTGGCAGCGATCGGTCGTCCTTACGGAATGGTTTTGGTCACCGGCCCCACCGGGTCGGGCAAGACGGTTTCGCT
CTATACCTGTTTGAACTTGCTCAACAAACCTGGTGTCAATATCGCCACGGCCGAAGATCCATCTGAAATCAATCTCCCAG
GAGTGAACCAAGTCAACGTCAACGAAAAGGCCGGGCTCACCTTTGCGGTGGCTCTCAAGTCTTTTCTTCGTCAGGATCCG
GACATCATCATGGTGGGTGAAATTCGCGATCTGGAGACCGCCGATATTTCTATCAAAGCGGCGCAGACGGGACACCTTGT
TCTTTCAACCTTGCATACCAATGACGCGCCCACCACATTAACTCGCATGCGCAATATGGGAATTGCGCCCTTCAATATTG
CGTCCAGCGTTATATTGATCACGGCCCAGAGACTGGCCCGTCGGCTTTGCCCTGTTTGCAAGACACCTGCTGATATTCCC
CATGAAACACTGGTGGATGCAGGTTTTCCTGAGCATGAGATCGATGGCTCTTGGGTAACCTACCGCCCTGTTGGCTGCTC
TGCCTGCAACAACGGATACAAGGGCCGTCTGGGGATTTATCAGGTCATGCCAATTACCGAAGAGATTCAGCGCATCATCC
TGCGCGACGGCAGCGCGCTGGAAATTGCGGAACAAGCCAAACGGGAAGGCGTACGTTCTTTGCGTGACGCGGGTCTGCAT
AAAGCGAAATTGGGCCTCACTTCATTGGAAGAAGTGCTGGCAGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.24

97.924

0.531
  pilB Acinetobacter baumannii D1279779

52.962

99.308

0.526
  pilF Neisseria gonorrhoeae MS11

52.743

97.751

0.516
  pilB Legionella pneumophila strain ERS1305867

49.468

97.578

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

45.406

97.924

0.445
  pilB Vibrio campbellii strain DS40M4

44.991

98.443

0.443
  pilB Vibrio cholerae strain A1552

45.863

96.194

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.908

100

0.394
  pilF Thermus thermophilus HB27

39.51

98.962

0.391

Multiple sequence alignment