Detailed information NTG0232110

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: CCX85_RS04405
• Genome accession: NZ_CP021640
• Coordinates: 868477..869313 (+)
• Length: 278 a.a.
• NCBI ID: WP_011054501.1
• Uniprot ID: A0A5S4TKN4
• Organism: Streptococcus pyogenes strain JS12
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 863477..874313

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CCX85_RS04375 (CCX85_04375)	-	863965..864399 (+)	435	WP_023605112.1	hypothetical protein	-
CCX85_RS04380 (CCX85_04380)	-	864449..865360 (-)	912	WP_023610321.1	diacylglycerol/lipid kinase family protein	-
CCX85_RS04385 (CCX85_04385)	trhA	865477..866127 (-)	651	WP_002984629.1	PAQR family membrane homeostasis protein TrhA	-
CCX85_RS04390 (CCX85_04390)	-	866124..866564 (-)	441	WP_011017769.1	DUF1836 domain-containing protein	-
CCX85_RS04395 (CCX85_04395)	ylqF	866783..867631 (+)	849	WP_023610309.1	ribosome biogenesis GTPase YlqF	-
CCX85_RS04400 (CCX85_04400)	-	867621..868412 (+)	792	WP_023610315.1	ribonuclease HII	-
CCX85_RS04405 (CCX85_04405)	dprA	868477..869313 (+)	837	WP_011054501.1	DNA-processing protein DprA	Machinery gene
CCX85_RS04410 (CCX85_04410)	topA	869420..871549 (+)	2130	WP_023610295.1	type I DNA topoisomerase	-
CCX85_RS04415 (CCX85_04415)	trmFO	871684..873030 (+)	1347	WP_023610318.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-

Sequence

Protein

• Length: 278 a.a.
• Molecular weight: 31087.14 Da
• Isoelectric Point: 9.4248

>NTDB_id=232110 CCX85_RS04405 WP_011054501.1 868477..869313(+) (dprA) [Streptococcus pyogenes strain JS12]
MNHFELYKLKKAGLTNKNILNILDYQKHQEKSLSLRDMAVVSGCKHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYP
MALKEIYNPPVLLFFQGNLDLLEKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPREEALSYHFPERNRIIAGLSRGILVVEAKNRSGSLITCQIGIEEGR
DIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILSEY

Nucleotide

• Length: 837 bp

>NTDB_id=232110 CCX85_RS04405 WP_011054501.1 868477..869313(+) (dprA) [Streptococcus pyogenes strain JS12]
GTGAATCATTTTGAACTTTATAAGCTAAAGAAAGCTGGACTGACTAATAAAAACATTCTCAATATTCTTGACTACCAAAA
ACACCAGGAAAAATCGTTGTCACTTCGAGATATGGCCGTTGTTTCTGGTTGTAAGCATCCGTCTCACTTTATAGAAGCCT
ATAAGCAGTTAGATATTCAAAAATTAAAAATGGAATTTAAACAATTTCCTAGTATTTCTATCTTAGATAAGCATTACCCA
ATGGCATTGAAAGAAATATACAATCCACCTGTCCTCTTGTTTTTTCAGGGAAATTTAGACCTTCTTGAGAAACCTAAATT
AGCCATTGTCGGCTCCAGACGCTCAAGCGATACCGGAGTAAAGTCTGTCCGTAAAATTCTTAAAGAACTCGGGAACCGTT
TTGTGATTGTTAGCGGACTTGCTCGAGGTATCGACACTAGTGCTCATTTAGCCTGCCTTAAAAATGGGGGACAAACCATC
GCTATTATTGGAACAGGGTTGGATCGCTTTTACCCTAAAGAAAATAGGGAGTTGCAAACTTTCTTAGGGAAAAATCATCT
TGTGCTAACAGAATACGGTCCAAGAGAAGAAGCTTTATCTTATCACTTTCCAGAACGGAATCGTATTATCGCAGGCCTTA
GCCGAGGTATTCTTGTCGTTGAAGCAAAAAATCGTTCAGGTTCCTTGATTACTTGTCAAATTGGGATAGAAGAAGGCCGA
GACATTTTTGCTGTCCCAGGAAACATTTTGGACGGGAAATCCGAAGGTTGCCTTCAGTTAATTAAAGAGGGAGCAACATG
CGTCACATCGGGCATGGATATCCTTTCAGAGTACTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

• DNA recombination-mediator protein A (DNA_processg_A) (68-269)

3D structure

• AlphaFold DB: A0A5S4TKN4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	67.266	100	0.673
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae D39	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae R6	60.072	100	0.601
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	60.072	100	0.601
dprA/cilB/dalA	Streptococcus mitis SK321	60.072	100	0.601
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	59.712	100	0.597
dprA	Lactococcus lactis subsp. cremoris KW2	57.194	100	0.572
dprA	Legionella pneumophila strain ERS1305867	42.857	85.612	0.367