Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSPPH_RS04205 Genome accession   NC_005773
Coordinates   980700..982394 (-) Length   564 a.a.
NCBI ID   WP_011167728.1    Uniprot ID   -
Organism   Pseudomonas savastanoi pv. phaseolicola 1448A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 975700..987394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPPH_RS04185 (PSPPH_0816) yacG 977780..977989 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  PSPPH_RS04190 (PSPPH_0817) coaE 977986..978609 (-) 624 WP_011167725.1 dephospho-CoA kinase -
  PSPPH_RS04195 (PSPPH_0818) pilD 978606..979478 (-) 873 WP_011167726.1 prepilin peptidase Machinery gene
  PSPPH_RS04200 (PSPPH_0819) pilC 979480..980697 (-) 1218 WP_011167727.1 type II secretion system F family protein Machinery gene
  PSPPH_RS04205 (PSPPH_0820) pilB 980700..982394 (-) 1695 WP_011167728.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSPPH_RS27995 (PSPPH_0821) - 982619..983014 (+) 396 WP_011167729.1 pilin -
  PSPPH_RS04215 (PSPPH_0822) - 983579..984838 (+) 1260 WP_004663377.1 IS256-like element ISPsy17 family transposase -
  PSPPH_RS28005 (PSPPH_0823) - 984881..986107 (-) 1227 Protein_844 polysaccharide biosynthesis C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62566.84 Da        Isoelectric Point: 6.3332

>NTDB_id=23210 PSPPH_RS04205 WP_011167728.1 980700..982394(-) (pilB) [Pseudomonas savastanoi pv. phaseolicola 1448A]
MTDAVLTGLAKQLVHAELLTESVAQQAYQQARRNKISLVSYLVQSKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETFLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=23210 PSPPH_RS04205 WP_011167728.1 980700..982394(-) (pilB) [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGACTGATGCTGTCCTCACTGGTTTGGCCAAACAGTTGGTCCACGCCGAGCTACTTACCGAATCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGCCGTAACAAGATTTCGTTGGTGAGCTATCTGGTCCAGAGCAAATTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGATCAGTTTGGCGTGCCCTTCATGGACCTCGCCAGCCTGGACAAAGAAAGCCAGCCTAAAGGCCTG
GTGAGTGAAAAGCTGGTTCGACAACATCATGCACTGCCGCTGTGGCGGCGCGGCAATAAACTATTTATCGGGATTTCGGA
CCCGACCAATCATCAGGCGGTGACTGATATTCAGTTCAGCACGGGCCTGAACACCGAAGCTATTCTGGTCGAAGATGACA
AGCTGACTATTGCTATAGATCGCTTTTTTGACAGTGATAGCGGTCTGGGCAATCTGGAAGATGTAGATCTGGGTCTGGAC
ATCGAACCGGCTGACGGCAAGGAAACCTTCCTTGCTACTCAGAGTGACGCTGACGACGCCCCGGTAGTACGCTTCGTCAA
CAAGATGCTGATGGATGCTATCCGATTAGGGTCATCCGACTTGCACTTCGAACCCTACGAAAAGATCTTCCGCGTGCGGT
TGCGTACTGACGGCATCTTGCATGAAGTCGCCAGACCGCCGATTCATCTGGCAAACCGTATTGCCGCGCGCTTGAAAGTC
ATGGCTAGCCTAGATATCTCGGAACGGCGTAAACCACAGGACGGACGTGTCAAGTTACGTGTGTCCAAAACCAAAGCCAT
CGATTTTCGTATGAATACCTTACCCACGCTATGGGGAGAGAAGATCGTAATGCGGATCCTCGATCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTACGAGCCAGAACAAAAAGCGCTGTATCTTGAAGCGCTGAAGCAACCACAAGGCATGATT
CTTGTGACCGGCCCCACCGGCTCGGGCAAAACAGTTTCGCTATACACCGGCCTGAACATTCTTAACACTGTGGACATCAA
CATTTCCACAGCTGAAGACCCAGTAGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGACTGG
ACTTTTCTCAGGCATTGCGCGCATTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATACGAGATCTGGAAACC
GCCGAAATCGCCATCAAAGCCTCTCAGACCGGCCATATGGTTTTATCTACTCTGCATACTAATAGTGCCGCAGAAACCCT
TACTCGCTTGCATCATATGGGCGTCGCAGCGTTCAACATCGCTACGGCGATTAACCTCATCATTGCCCAGCGGCTGGCGC
GCAAGCTATGCAGCCATTGCAAGAAAGAGCTCGACATACCCCGGGAAACGCTCATCCAGGAGGGGTTTCCAGAGGAGAAG
ATCGGTACGTTTAAAATCTATGGCCCAGTAGGTTGTGAGCATTGCAACGGCGGCTACAGGGGCAGGGTGGGCATTTACGA
AGTAGTCAAGAAAACACCAGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGACTGTCGAAGGCCATGCAAGGTATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.582
  pilB Acinetobacter baylyi ADP1

56.714

100

0.569
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

50.263

100

0.509
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio campbellii strain DS40M4

49.468

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.221

99.645

0.481
  pilF Thermus thermophilus HB27

39.029

98.582

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369

Multiple sequence alignment