Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CBP36_RS15470 Genome accession   NZ_CP021366
Coordinates   3335111..3336847 (+) Length   578 a.a.
NCBI ID   WP_086928036.1    Uniprot ID   A0A240UEX3
Organism   Acidovorax carolinensis strain P4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3330111..3341847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP36_RS15450 (CBP36_15440) - 3330622..3330981 (+) 360 WP_236748739.1 hypothetical protein -
  CBP36_RS15455 (CBP36_15445) istB 3331007..3331822 (-) 816 WP_026437709.1 IS21-like element helper ATPase IstB -
  CBP36_RS15460 (CBP36_15450) istA 3331815..3333338 (-) 1524 WP_086927645.1 IS21 family transposase -
  CBP36_RS15465 (CBP36_15455) - 3333396..3334286 (-) 891 WP_204247568.1 hypothetical protein -
  CBP36_RS15470 (CBP36_15460) pilB 3335111..3336847 (+) 1737 WP_086928036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CBP36_RS15475 (CBP36_15465) pilC 3336868..3338088 (+) 1221 WP_086913249.1 type II secretion system F family protein Machinery gene
  CBP36_RS15480 (CBP36_15470) - 3338088..3338966 (+) 879 WP_086928037.1 prepilin peptidase -
  CBP36_RS15485 (CBP36_15475) coaE 3338969..3339577 (+) 609 WP_086928038.1 dephospho-CoA kinase -
  CBP36_RS15490 (CBP36_15480) zapD 3339663..3340418 (+) 756 WP_086928039.1 cell division protein ZapD -
  CBP36_RS15495 (CBP36_15485) - 3340432..3340650 (+) 219 WP_086928040.1 DNA gyrase inhibitor YacG -
  CBP36_RS15500 (CBP36_15490) - 3340741..3341160 (-) 420 WP_269466978.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62916.20 Da        Isoelectric Point: 5.3457

>NTDB_id=230213 CBP36_RS15470 WP_086928036.1 3335111..3336847(+) (pilB) [Acidovorax carolinensis strain P4]
MAAVDTAQKEASSVALPGLGRALMSAGKLTQKSAEDIYKKSQSSRTSFIAELTGSGVVSASDLAHTVSAVFGAPLLDLDA
IDPQRLPKDLLDAKICSAYRVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGMDWIIAEYDKLSRLVEISTKSTSETM
DTLISGGGDFEFDDVAVEEAAESNEATTEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEAVEKE
RLLAAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKTPADIP
HEALVDAGYPEDELDGSWVTYRPVGCSACNNGYKGRLGIYQVMPITEEIQRIILRDGSAMEIAEQAKREGVRSLREAGLH
KAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=230213 CBP36_RS15470 WP_086928036.1 3335111..3336847(+) (pilB) [Acidovorax carolinensis strain P4]
ATGGCCGCTGTCGATACTGCCCAGAAAGAAGCTTCCTCAGTAGCCCTCCCGGGATTGGGCCGCGCGCTGATGTCCGCGGG
AAAGCTGACACAAAAATCAGCCGAGGATATTTACAAAAAGTCACAATCAAGCCGCACCAGCTTTATCGCTGAACTGACCG
GATCAGGTGTCGTTTCGGCCTCGGATTTGGCGCACACCGTCTCGGCTGTTTTTGGCGCGCCTTTGCTGGATCTGGATGCC
ATAGATCCGCAGCGTCTTCCCAAAGATCTGCTGGACGCCAAAATTTGCAGCGCCTACCGGGTGGTTGTTCTCAGCAAAAG
AAATAATCGACTCATCGTTGCCACGGCGGATCCAACGGATCAGGAAGCCGCAGAGAAAATCAAATTCACCACCCAAATGG
GAATGGACTGGATTATTGCCGAATACGACAAATTAAGTCGTCTGGTAGAAATCAGCACAAAATCCACCAGCGAAACCATG
GATACGCTGATCAGCGGTGGAGGCGATTTTGAATTCGACGATGTGGCTGTCGAAGAGGCTGCGGAGAGCAATGAAGCAAC
AACCGAAGTTGAAGATGCTCCCATTGTCAAGTTTCTGCACAAAATGCTGCTGGATGCCTTCAACATGCGGGCATCGGATC
TGCATTTTGAACCTTATGAGCACCAGTATCGAGTACGGTTTCGTATTGACGGCGAGTTGCGCGAGATTGCATCGCCTCCG
ATTGCCATCAAGGATAAATTAGCCTCCCGCATCAAGGTTATATCGCGCCTCGATATTTCCGAGAAACGGGTGCCCCAGGA
TGGACGAATGAAACTGAAAGTGGGACCGGACCGGGTCATCGACTTTCGGGTCAGCACCTTGCCCACGCTGTTTGGTGAAA
AAATCGTGATCCGTATTCTGGATCCCAGCAGCGCAAAATTGGGCATCGATGCCCTCGGATATGAGGCCGTAGAGAAAGAG
CGCCTGCTGGCGGCTATCGGGCGCCCTTACGGAATGGTTTTGGTCACCGGCCCCACCGGGTCGGGCAAAACGGTTTCGCT
CTATACCTGTTTGAATCTGCTCAATAAGCCCGGCGTCAATATCGCCACCGCCGAAGACCCCTCTGAAATCAACCTCCCAG
GGGTGAACCAGGTCAACGTCAACGAAAAGGCCGGGCTCACCTTTGCGGTGGCCCTCAAGTCATTTCTGCGTCAGGATCCC
GACATCATCATGGTGGGGGAAATTCGCGATCTGGAAACCGCTGACATTTCCATCAAGGCGGCACAGACGGGACACCTCGT
ACTGTCCACATTGCATACCAATGACGCCCCCACCACGTTGACACGCATGCGCAACATGGGAATTGCGCCCTTCAATATTG
CATCCAGTGTCATATTGATCACGGCCCAGCGTCTGGCCCGTCGGCTTTGCCCCGTGTGCAAGACACCAGCCGATATTCCA
CATGAAGCACTCGTGGATGCCGGCTACCCCGAGGATGAGCTCGATGGTTCCTGGGTAACCTACCGCCCTGTTGGCTGCTC
TGCCTGCAATAACGGGTACAAGGGTCGCTTGGGGATTTATCAGGTCATGCCCATCACTGAAGAAATTCAGCGCATCATCC
TGCGCGACGGCAGCGCGATGGAAATTGCGGAGCAGGCAAAGCGGGAAGGCGTGCGCTCTTTACGTGAAGCGGGCCTGCAT
AAAGCGAAATTGGGCCTCACTTCCCTGGAAGAAGTGCTGGCAGTAACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240UEX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.749

100

0.531
  pilB Acinetobacter baylyi ADP1

53.684

98.616

0.529
  pilF Neisseria gonorrhoeae MS11

52.743

97.751

0.516
  pilB Legionella pneumophila strain ERS1305867

48.936

97.578

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

44.561

98.616

0.439
  pilB Vibrio campbellii strain DS40M4

44.602

97.751

0.436
  pilB Vibrio cholerae strain A1552

44.909

95.156

0.427
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.978

92.734

0.389
  pilF Thermus thermophilus HB27

38.986

98.962

0.386

Multiple sequence alignment