Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CBP33_RS15040 Genome accession   NZ_CP021359
Coordinates   3212963..3214699 (+) Length   578 a.a.
NCBI ID   WP_086913248.1    Uniprot ID   -
Organism   Acidovorax carolinensis strain NA2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3207963..3219699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP33_RS15010 (CBP33_15000) proB 3208497..3209639 (-) 1143 WP_086913246.1 glutamate 5-kinase -
  CBP33_RS15015 (CBP33_15005) cgtA 3209733..3210803 (-) 1071 WP_086913247.1 Obg family GTPase CgtA -
  CBP33_RS15020 (CBP33_15010) rpmA 3210971..3211228 (-) 258 WP_008905145.1 50S ribosomal protein L27 -
  CBP33_RS15025 (CBP33_15015) rplU 3211244..3211555 (-) 312 WP_024814187.1 50S ribosomal protein L21 -
  CBP33_RS15030 (CBP33_15020) - 3211786..3212715 (+) 930 WP_086914179.1 polyprenyl synthetase family protein -
  CBP33_RS15040 (CBP33_15030) pilB 3212963..3214699 (+) 1737 WP_086913248.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CBP33_RS15045 (CBP33_15035) pilC 3214720..3215940 (+) 1221 WP_086913249.1 type II secretion system F family protein Machinery gene
  CBP33_RS15050 (CBP33_15040) - 3215940..3216818 (+) 879 WP_086913250.1 prepilin peptidase -
  CBP33_RS15055 (CBP33_15045) coaE 3216821..3217429 (+) 609 WP_086913251.1 dephospho-CoA kinase -
  CBP33_RS15060 (CBP33_15050) zapD 3217515..3218270 (+) 756 WP_086913252.1 cell division protein ZapD -
  CBP33_RS15065 (CBP33_15055) - 3218284..3218502 (+) 219 WP_086913253.1 DNA gyrase inhibitor YacG -
  CBP33_RS15070 (CBP33_15060) - 3218594..3219013 (-) 420 WP_086913254.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62884.14 Da        Isoelectric Point: 5.3457

>NTDB_id=230162 CBP33_RS15040 WP_086913248.1 3212963..3214699(+) (pilB) [Acidovorax carolinensis strain NA2]
MAAVDTAQKEASSVALPGLGRALMSAGKLTQKSAEDIYKKSQSSRTSFIAELTGSGVVSASDLAHTVSAVFGAPLLDLDA
IDPQRLPKDLLDAKICSAYRVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLSRLVEISTKSTSETM
DTLISGGGDFEFDDVAVEEAAESNEATTEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEAVEKE
RLLAAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKTPADIP
HEALVDAGYPEDELDGSWVTYRPVGCSACNNGYKGRLGIYQVMPITEEIQRIILRDGSAMEIAEQAKREGVRSLREAGLH
KAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=230162 CBP33_RS15040 WP_086913248.1 3212963..3214699(+) (pilB) [Acidovorax carolinensis strain NA2]
ATGGCCGCTGTCGATACTGCCCAGAAAGAAGCTTCCTCAGTAGCCCTCCCGGGATTGGGCCGCGCGCTGATGTCCGCGGG
AAAGCTGACACAAAAATCAGCCGAGGATATTTACAAAAAGTCACAATCAAGCCGCACCAGCTTTATCGCTGAGCTGACCG
GATCAGGTGTCGTTTCGGCCTCGGATTTGGCGCACACCGTCTCGGCTGTTTTTGGCGCGCCTTTGCTGGATCTGGATGCC
ATAGATCCGCAGCGTCTTCCCAAAGATCTGCTGGACGCCAAAATTTGCAGCGCCTACCGGGTGGTTGTTCTCAGCAAAAG
AAATAATCGACTCATCGTTGCCACGGCGGATCCAACGGATCAGGAAGCCGCAGAGAAAATCAAATTCACCACCCAAATGG
GGGTGGACTGGATTATTGCCGAATACGACAAATTAAGTCGTCTGGTAGAAATCAGCACAAAATCCACCAGCGAAACCATG
GATACGCTGATCAGCGGTGGAGGCGATTTTGAATTCGACGATGTGGCTGTCGAAGAGGCTGCGGAGAGCAATGAAGCGAC
AACCGAAGTTGAAGATGCTCCCATCGTCAAGTTTCTGCACAAAATGCTGCTGGATGCCTTCAATATGCGGGCATCGGATC
TGCATTTTGAACCTTATGAGCACCAGTATCGAGTACGGTTTCGTATTGACGGAGAGTTGCGCGAGATTGCATCGCCTCCG
ATTGCCATCAAGGATAAATTAGCCTCCCGCATCAAGGTTATATCGCGCCTCGATATTTCCGAGAAACGGGTGCCCCAGGA
TGGGCGAATGAAACTGAAAGTGGGACCGGACCGGGTCATCGACTTTCGTGTCAGCACCTTGCCGACGCTGTTTGGTGAAA
AAATCGTGATCCGCATTCTGGATCCCAGCAGCGCAAAATTGGGCATCGATGCCCTCGGATATGAGGCCGTAGAGAAAGAG
CGCCTGCTGGCGGCTATCGGGCGCCCTTACGGAATGGTTTTGGTCACCGGCCCCACCGGGTCGGGCAAAACGGTTTCGCT
CTATACCTGTTTGAATCTGCTCAATAAGCCTGGCGTCAATATCGCCACCGCCGAAGACCCCTCTGAAATCAACCTCCCAG
GGGTGAACCAGGTCAACGTCAACGAAAAGGCCGGGCTCACCTTTGCGGTGGCCCTCAAGTCATTTCTGCGTCAAGATCCC
GACATCATCATGGTGGGTGAAATTCGCGATCTGGAAACCGCTGACATTTCCATCAAGGCGGCACAAACGGGACACCTCGT
ACTGTCCACATTGCATACCAATGACGCCCCCACCACGTTGACACGCATGCGCAACATGGGAATTGCGCCCTTCAATATTG
CATCCAGTGTCATATTGATCACGGCCCAGCGTCTGGCCCGTCGGCTTTGCCCCGTGTGCAAGACACCAGCCGATATTCCA
CATGAAGCACTCGTGGATGCCGGCTACCCCGAGGATGAGCTCGATGGTTCCTGGGTAACCTACCGCCCTGTTGGCTGCTC
TGCCTGCAATAACGGGTACAAGGGTCGCTTGGGGATTTATCAGGTCATGCCCATCACCGAAGAAATTCAGCGCATCATCC
TGCGCGACGGCAGCGCGATGGAAATTGCGGAGCAGGCAAAGCGGGAAGGCGTGCGTTCTTTGCGTGAAGCGGGCCTGCAT
AAAGCGAAATTGGGCCTCACTTCCCTGGAAGAAGTGCTGGCAGTAACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.921

100

0.533
  pilB Acinetobacter baylyi ADP1

53.86

98.616

0.531
  pilF Neisseria gonorrhoeae MS11

52.92

97.751

0.517
  pilB Legionella pneumophila strain ERS1305867

48.936

97.578

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

44.737

98.616

0.441
  pilB Vibrio campbellii strain DS40M4

44.779

97.751

0.438
  pilB Vibrio cholerae strain A1552

45.091

95.156

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.978

92.734

0.389
  pilF Thermus thermophilus HB27

38.986

98.962

0.386

Multiple sequence alignment