Detailed information NTG0230099

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB987_RS09045
• Genome accession: NZ_CP021347
• Coordinates: 1855705..1856763 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain B8300
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1850705..1861763

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB987_RS09030 (AB987_1826)	-	1851811..1852143 (+)	333	WP_000169598.1	hypothetical protein	-
AB987_RS09035 (AB987_1827)	-	1852146..1852970 (+)	825	WP_002068938.1	putative RNA methyltransferase	-
AB987_RS09040 (AB987_1828)	ponA	1852988..1855543 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
AB987_RS09045 (AB987_1829)	pilM	1855705..1856763 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
AB987_RS09050 (AB987_1830)	pilN	1856763..1857404 (+)	642	WP_000201224.1	PilN domain-containing protein	Machinery gene
AB987_RS09055 (AB987_1831)	pilO	1857401..1858141 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
AB987_RS09060 (AB987_1832)	pilP	1858152..1858679 (+)	528	WP_004833663.1	pilus assembly protein PilP	Machinery gene
AB987_RS09065 (AB987_1833)	pilQ	1858742..1860907 (+)	2166	WP_001017029.1	type IV pilus secretin PilQ family protein	Machinery gene
AB987_RS09070 (AB987_1834)	aroK	1860919..1861488 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=230099 AB987_RS09045 WP_002014066.1 1855705..1856763(+) (pilM) [Acinetobacter baumannii strain B8300]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=230099 AB987_RS09045 WP_002014066.1 1855705..1856763(+) (pilM) [Acinetobacter baumannii strain B8300]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGACTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423