Detailed information NTG0230046

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB895_RS05825
• Genome accession: NZ_CP021342
• Coordinates: 1220842..1221900 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain B8342
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1215842..1226900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB895_RS05800 (AB895_1178)	aroK	1216117..1216686 (-)	570	WP_000643712.1	shikimate kinase AroK	-
AB895_RS05805 (AB895_1179)	pilQ	1216698..1218863 (-)	2166	WP_031993779.1	type IV pilus secretin PilQ	Machinery gene
AB895_RS05810 (AB895_1180)	pilP	1218926..1219453 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
AB895_RS05815 (AB895_1181)	pilO	1219464..1220204 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
AB895_RS05820 (AB895_1182)	pilN	1220201..1220842 (-)	642	WP_004742152.1	PilN domain-containing protein	Machinery gene
AB895_RS05825 (AB895_1183)	pilM	1220842..1221900 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
AB895_RS05830 (AB895_1184)	ponA	1222063..1224618 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
AB895_RS05835 (AB895_1185)	-	1224636..1225460 (-)	825	WP_004742150.1	putative RNA methyltransferase	-
AB895_RS05840 (AB895_1186)	-	1225463..1225795 (-)	333	WP_000169599.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=230046 AB895_RS05825 WP_002014066.1 1220842..1221900(-) (pilM) [Acinetobacter baumannii strain B8342]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=230046 AB895_RS05825 WP_002014066.1 1220842..1221900(-) (pilM) [Acinetobacter baumannii strain B8342]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423