Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B9G99_RS16100 Genome accession   NZ_CP021323
Coordinates   3472540..3474276 (+) Length   578 a.a.
NCBI ID   WP_086623088.1    Uniprot ID   A0A2Z2HA06
Organism   Kushneria konosiri strain X49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3467540..3479276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9G99_RS16080 (B9G99_16070) - 3468080..3469006 (+) 927 WP_086623084.1 recombination-associated protein RdgC -
  B9G99_RS16085 (B9G99_16075) - 3469036..3470208 (+) 1173 WP_086623085.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  B9G99_RS16090 (B9G99_16080) - 3470281..3471756 (-) 1476 WP_086623086.1 TrkH family potassium uptake protein -
  B9G99_RS16095 (B9G99_16085) - 3471880..3472353 (-) 474 WP_086623087.1 pilin -
  B9G99_RS16100 (B9G99_16090) pilB 3472540..3474276 (+) 1737 WP_086623088.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B9G99_RS16105 (B9G99_16095) - 3474285..3475526 (+) 1242 WP_086623089.1 type II secretion system F family protein -
  B9G99_RS16110 (B9G99_16100) - 3475546..3476397 (+) 852 WP_269466788.1 prepilin peptidase -
  B9G99_RS16115 (B9G99_16105) coaE 3476454..3477062 (+) 609 WP_086623090.1 dephospho-CoA kinase -
  B9G99_RS16120 (B9G99_16110) yacG 3477059..3477277 (+) 219 WP_086623091.1 DNA gyrase inhibitor YacG -
  B9G99_RS16125 (B9G99_16115) - 3477354..3478298 (-) 945 WP_086623531.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63795.13 Da        Isoelectric Point: 5.5205

>NTDB_id=229839 B9G99_RS16100 WP_086623088.1 3472540..3474276(+) (pilB) [Kushneria konosiri strain X49]
MSDALSQLSANPLRSLAHQLVEDGLLDADHALALEQRAQQEKRPLLRLITEETDVSPRDAIQSAGWAFGLGCVDLDAIPV
ARLPPLQGLPESMIRRLGVLPLMRQERCLIVAVDDPSRLPELDELQFVIGMSVEGMLAPAKQLAARLEQYLDMHGEGALA
LLEGHNAIETLAQEESDLALDQTEDASFQSSDDAPVVRFVKQILLDGIRRGASDIHFEPFDESFRIRFRVDGILMEASRP
PAQLRQRIAARIKVMAKLDISERRLPQDGNLRLKLSSRRHVEFRVNTLPTLFGEKIVMRLLDPDSARMGIDALGFTAEQR
AIFEQAIARPQGMILSTGPTGSGKTVTLYTALNLLNTEARNIATAEDPVEIKLEGINQVNVRPSIGFDFAAALRAFLRQD
PDVVMVGEIRDLETAEVAIKAAQTGHLVLSTLHTNSAAETLTRLGNMGVAGFNIATSISLIIAQRLARRLCERCRQPVTL
PREVLRHQGLNDEEIDQATLYQAVGCEHCTHGYRGRVGIYEMVPISSAMSHLIMQDAGAMALAEQARREGHHDLRRSGLH
KVLTGLTTLEELNRTIQE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=229839 B9G99_RS16100 WP_086623088.1 3472540..3474276(+) (pilB) [Kushneria konosiri strain X49]
ATGTCGGATGCTCTCAGCCAACTCTCAGCCAACCCTCTGCGAAGCCTCGCCCATCAGCTGGTCGAGGATGGATTGCTGGA
CGCCGACCATGCGCTGGCCCTGGAGCAACGGGCGCAGCAGGAAAAGCGCCCGCTGCTCAGGCTGATTACCGAAGAAACCG
ACGTATCACCACGCGACGCGATTCAGAGTGCCGGCTGGGCCTTCGGACTCGGCTGTGTGGATCTGGATGCGATTCCGGTC
GCGCGTCTGCCGCCGCTACAGGGTCTGCCGGAATCGATGATCCGCCGGCTGGGCGTGCTGCCTTTGATGCGTCAGGAGCG
CTGTTTGATCGTGGCGGTGGATGACCCCTCTCGCCTGCCCGAACTCGATGAACTTCAGTTCGTGATTGGCATGAGCGTGG
AAGGCATGCTGGCGCCGGCCAAACAGCTTGCCGCCAGACTCGAGCAGTATCTGGACATGCACGGCGAAGGGGCGCTGGCG
CTGCTGGAGGGTCACAACGCCATTGAGACACTGGCACAGGAAGAAAGCGATCTTGCGCTGGATCAAACAGAAGACGCTTC
ATTTCAATCCAGCGACGACGCGCCGGTGGTGCGTTTCGTCAAGCAGATCCTGCTCGACGGTATTCGGCGCGGCGCCTCGG
ACATCCATTTCGAGCCGTTTGACGAGAGCTTTCGAATCCGCTTTCGCGTCGATGGCATTCTCATGGAAGCTTCCCGACCG
CCGGCACAGCTTCGCCAGCGTATTGCAGCCCGCATCAAGGTGATGGCCAAGCTGGACATCTCCGAGCGACGCCTGCCCCA
GGATGGCAACCTGCGCCTGAAGCTGTCGAGCCGACGCCACGTTGAATTTCGTGTCAACACGCTACCCACCCTGTTTGGTG
AAAAGATTGTCATGCGCCTGCTGGACCCGGACAGCGCCCGTATGGGCATCGATGCACTGGGCTTCACGGCCGAGCAGCGC
GCCATCTTTGAACAGGCCATCGCCCGGCCCCAGGGCATGATTCTATCGACCGGCCCCACCGGCAGCGGCAAGACGGTGAC
GCTTTATACGGCTCTGAATCTGCTCAATACCGAGGCGCGCAATATCGCTACGGCCGAGGACCCGGTCGAAATCAAGCTGG
AAGGCATCAACCAGGTCAACGTACGACCCAGTATCGGCTTTGATTTTGCCGCCGCCCTGCGCGCTTTTCTGCGTCAGGAC
CCGGATGTGGTGATGGTGGGGGAAATTCGTGACCTGGAAACCGCCGAAGTCGCCATCAAGGCGGCCCAGACCGGACATCT
GGTGCTATCGACACTACACACCAACTCTGCCGCCGAAACCCTGACTCGTCTGGGCAACATGGGCGTGGCCGGCTTCAACA
TTGCCACCTCCATCAGCCTGATCATCGCTCAGCGACTGGCACGCCGCCTGTGTGAACGCTGCCGACAGCCGGTCACCCTG
CCCCGCGAAGTGCTCAGACATCAGGGACTCAATGACGAGGAAATTGATCAGGCCACGCTGTATCAGGCCGTAGGCTGTGA
GCACTGCACGCATGGCTACCGAGGACGTGTGGGGATTTATGAAATGGTGCCGATATCCTCTGCCATGAGCCATTTGATCA
TGCAGGATGCCGGTGCCATGGCGCTGGCCGAGCAGGCGCGACGAGAAGGTCATCACGACCTGCGTCGCAGCGGTTTGCAC
AAGGTGCTGACCGGACTGACCACGCTGGAAGAACTCAACCGGACCATTCAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(20-153)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z2HA06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.352

98.27

0.495
  pilB Acinetobacter baylyi ADP1

47.887

98.27

0.471
  pilB Vibrio cholerae strain A1552

52.216

89.792

0.469
  pilB Acinetobacter baumannii D1279779

46.312

100

0.467
  pilB Vibrio campbellii strain DS40M4

51.751

88.927

0.46
  pilF Neisseria gonorrhoeae MS11

46.691

96.713

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

50.292

88.754

0.446
  pilF Thermus thermophilus HB27

39.442

99.135

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.902

100

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

39.377

94.464

0.372

Multiple sequence alignment