Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA921_RS04935 Genome accession   NZ_CP021283
Coordinates   1070574..1071638 (+) Length   354 a.a.
NCBI ID   WP_011215094.1    Uniprot ID   Q5WXY1
Organism   Legionella pneumophila subsp. pneumophila strain Allentown     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1065574..1076638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA921_RS04925 - 1066614..1068998 (-) 2385 WP_061692111.1 penicillin-binding protein 1A -
  CA921_RS04930 ravI 1069150..1070157 (-) 1008 WP_061637670.1 Dot/Icm T4SS effector RavI -
  CA921_RS04935 pilM 1070574..1071638 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  CA921_RS04940 pilN 1071642..1072190 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  CA921_RS04945 pilO 1072204..1072803 (+) 600 WP_011215096.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA921_RS04950 pilP 1072800..1073384 (+) 585 WP_027266404.1 pilus assembly protein PilP Machinery gene
  CA921_RS04955 pilQ 1073388..1075484 (+) 2097 WP_011215098.1 type IV pilus secretin PilQ Machinery gene
  CA921_RS04960 aroK 1075929..1076456 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38760.78 Da        Isoelectric Point: 5.0367

>NTDB_id=229660 CA921_RS04935 WP_011215094.1 1070574..1071638(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Allentown]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229660 CA921_RS04935 WP_011215094.1 1070574..1071638(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Allentown]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGATGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAGTCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WXY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

100

100

1
  comM Acinetobacter nosocomialis M2

42.373

100

0.424
  pilM Acinetobacter baumannii D1279779

42.09

100

0.421
  comM Acinetobacter baylyi ADP1

40.678

100

0.407

Multiple sequence alignment