Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA926_RS05110 Genome accession   NZ_CP021265
Coordinates   1129374..1130438 (+) Length   354 a.a.
NCBI ID   WP_028379151.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. fraseri strain Los Angeles     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1124374..1135438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA926_RS05100 - 1125413..1127797 (-) 2385 WP_028377946.1 penicillin-binding protein 1A -
  CA926_RS05105 ravI 1127948..1128955 (-) 1008 WP_027227632.1 Dot/Icm T4SS effector RavI -
  CA926_RS05110 pilM 1129374..1130438 (+) 1065 WP_028379151.1 type IV pilus assembly protein PilM Machinery gene
  CA926_RS05115 pilN 1130442..1130990 (+) 549 WP_028379152.1 PilN domain-containing protein Machinery gene
  CA926_RS05120 pilO 1131004..1131603 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA926_RS05125 pilP 1131600..1132184 (+) 585 WP_028377949.1 pilus assembly protein PilP Machinery gene
  CA926_RS05130 pilQ 1132188..1134284 (+) 2097 WP_028377950.1 type IV pilus secretin PilQ Machinery gene
  CA926_RS05135 aroK 1134732..1135253 (+) 522 WP_027219629.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38810.84 Da        Isoelectric Point: 5.1378

>NTDB_id=229330 CA926_RS05110 WP_028379151.1 1129374..1130438(+) (pilM) [Legionella pneumophila subsp. fraseri strain Los Angeles]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEQVCVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDQEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKNSALLDVLIVASRAENVNSRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPASGQDKIIAIVDIGTNYTHLFALHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSYMSFGKKVNLDAINNVAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229330 CA926_RS05110 WP_028379151.1 1129374..1130438(+) (pilM) [Legionella pneumophila subsp. fraseri strain Los Angeles]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATTAGTTCTTCAGCAGTGAAAATTTTGGAAAT
TTCAGGTTCCGGAGAGCAAGTTTGTGTTGAAGGATATGGTCGTGAAGAAATACCCGCCTCTGCTCTTGAAGGGAATATGA
TCAAGGACGTCGATGTAGTAGCGAATTGTATTAAAAAATTGGTAGAAAGGTTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATTAGCAAAGTAGTGCAAATTAATGAAGGATTGACTGACCAGGAAATGGAAGAGCTTGT
TGTTATTGAAGCGGATAAGTATATTCCTTACCCAATAGATGAAATAAACCTTGATTTTGAAGTGTTAGGCCATTCTGCCA
AGAATTCAGCGCTGCTTGATGTTTTAATTGTGGCTTCAAGAGCAGAAAATGTTAATAGTCGTGTCGAGGCAGCTCATAGA
GCTGGCCTGGAGCCCAAAATCGTTGACGTTGAATCTTATGCGGTAGAACGTTCAGTGCAACAAATTGCGCAAGAATTGCC
AGCAAGTGGACAAGACAAAATTATAGCGATAGTAGATATTGGAACCAATTACACTCATTTATTTGCTTTGCATGGCATGC
GATTAATCTTTTCAAGAGAAGAAAAATTTGGTGGCATGCAGCTTGTTGAAGCTATTGCAGAACATTATCATATGAATTTA
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGCACTTTGCAGTTTTTTTATTCTACCAGTCAACATGGATTTGTTGACCATATTCTATTAG
CTGGAGGAATGGCAAGGCAACCAGGGTTGGCAGCGCTTATTCAAGAGCAATTAGGAGTGACTACTACAGTAGCAAATCCA
CTATCCTATATGTCTTTTGGTAAAAAAGTGAATTTGGATGCCATTAATAATGTCGCGCCTACACTGATGGTGGCTTGTGG
GTTAGCTCTAAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

96.045

100

0.96
  comM Acinetobacter nosocomialis M2

42.373

100

0.424
  pilM Acinetobacter baumannii D1279779

42.09

100

0.421
  comM Acinetobacter baylyi ADP1

40.113

100

0.401

Multiple sequence alignment