Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CA931_RS04950 Genome accession   NZ_CP021262
Coordinates   1056984..1057583 (+) Length   199 a.a.
NCBI ID   WP_027221266.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pascullei strain U8W (D-7160)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1051984..1062583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA931_RS04935 - 1053922..1054935 (-) 1014 WP_027221263.1 hypothetical protein -
  CA931_RS04940 pilM 1055354..1056418 (+) 1065 WP_027221264.1 pilus assembly protein PilM Machinery gene
  CA931_RS04945 pilN 1056422..1056970 (+) 549 WP_027221265.1 PilN domain-containing protein Machinery gene
  CA931_RS04950 pilO 1056984..1057583 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA931_RS04955 pilP 1057580..1058164 (+) 585 WP_027221267.1 pilus assembly protein PilP Machinery gene
  CA931_RS04960 pilQ 1058168..1060264 (+) 2097 WP_027221268.1 type IV pilus secretin PilQ Machinery gene
  CA931_RS04965 aroK 1060707..1061234 (+) 528 WP_032828804.1 shikimate kinase AroK -
  CA931_RS04970 aroB 1061221..1062324 (+) 1104 WP_027221270.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22441.13 Da        Isoelectric Point: 5.3305

>NTDB_id=229207 CA931_RS04950 WP_027221266.1 1056984..1057583(+) (pilO) [Legionella pneumophila subsp. pascullei strain U8W (D-7160)]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLDTLEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMGITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=229207 CA931_RS04950 WP_027221266.1 1056984..1057583(+) (pilO) [Legionella pneumophila subsp. pascullei strain U8W (D-7160)]
ATGGATACAGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGCCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGCATCAGTATATTGATAATTGGATTGGGATATTGGCTTATTGTTAAGCCAAATTTTGAGCAATTGGATACACTGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCCTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGAACCATGCTAAAACAACTCCCGGCAAAAAATGAAATGCCTGGGCTTTTGGAAGAAAT
ATCTAAAACAGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTAGCAAGATTTTTAAGTCGTGTTGCTGAAATGAACAGA
ATCGTGACTTTGCATGAATTTAGTGTTGAGGGGGTATCCTCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGGTATCACTGCTAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

97.487

100

0.975
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

38.22

95.98

0.367

Multiple sequence alignment