Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   CA931_RS04945 Genome accession   NZ_CP021262
Coordinates   1056422..1056970 (+) Length   182 a.a.
NCBI ID   WP_027221265.1    Uniprot ID   A0AAX2IVG0
Organism   Legionella pneumophila subsp. pascullei strain U8W (D-7160)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1051422..1061970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA931_RS04935 - 1053922..1054935 (-) 1014 WP_027221263.1 hypothetical protein -
  CA931_RS04940 pilM 1055354..1056418 (+) 1065 WP_027221264.1 pilus assembly protein PilM Machinery gene
  CA931_RS04945 pilN 1056422..1056970 (+) 549 WP_027221265.1 PilN domain-containing protein Machinery gene
  CA931_RS04950 pilO 1056984..1057583 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA931_RS04955 pilP 1057580..1058164 (+) 585 WP_027221267.1 pilus assembly protein PilP Machinery gene
  CA931_RS04960 pilQ 1058168..1060264 (+) 2097 WP_027221268.1 type IV pilus secretin PilQ Machinery gene
  CA931_RS04965 aroK 1060707..1061234 (+) 528 WP_032828804.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21223.01 Da        Isoelectric Point: 10.1404

>NTDB_id=229206 CA931_RS04945 WP_027221265.1 1056422..1056970(+) (pilN) [Legionella pneumophila subsp. pascullei strain U8W (D-7160)]
MTEINLLPWREQKREQEKKLFTTMLLIGIIVAAAIVFLMNSYVSHLISNQTQRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGIYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQSPVLTEIKK
IVEKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=229206 CA931_RS04945 WP_027221265.1 1056422..1056970(+) (pilN) [Legionella pneumophila subsp. pascullei strain U8W (D-7160)]
ATGACTGAGATTAATTTATTACCATGGCGTGAGCAAAAACGGGAACAGGAAAAGAAATTATTCACGACCATGTTATTGAT
AGGGATTATCGTTGCTGCGGCAATTGTTTTTTTAATGAATTCTTATGTTTCCCATTTAATAAGTAATCAAACGCAACGGA
ACCAGATTTTACAGAAAGAAATTACAGCCTTAGATGAGCAAATCAAGGAGATCAAGGCATTAAAGCAAATCAGAGAAGGT
CTGATTTCGAGAATGTCTATCGTGCAGAATCTGCAATCAACAAGAACTTTAATGGTTCATTTATTTGATGAGTTAATTAA
AATTACTCCTCCAGGAATTTACATAACCAAGATGCAAAGAGAACATGATGTCGTAACACTCTGGGGGTATTCTGAATCGA
ATACAAGTATTTCTATTTTGATGAGAAATATAGAGGCCAATGTCTGGATACAGAGTCCCGTACTTACTGAAATTAAAAAA
ATTGTTGAAAAAAAACAGTCTCCAAACAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

97.253

100

0.973

Multiple sequence alignment