Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA931_RS04940 Genome accession   NZ_CP021262
Coordinates   1055354..1056418 (+) Length   354 a.a.
NCBI ID   WP_027221264.1    Uniprot ID   A0AAX2IUM9
Organism   Legionella pneumophila subsp. pascullei strain U8W (D-7160)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1050354..1061418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA931_RS04930 - 1051387..1053771 (-) 2385 WP_027221262.1 penicillin-binding protein 1A -
  CA931_RS04935 - 1053922..1054935 (-) 1014 WP_027221263.1 hypothetical protein -
  CA931_RS04940 pilM 1055354..1056418 (+) 1065 WP_027221264.1 pilus assembly protein PilM Machinery gene
  CA931_RS04945 pilN 1056422..1056970 (+) 549 WP_027221265.1 PilN domain-containing protein Machinery gene
  CA931_RS04950 pilO 1056984..1057583 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA931_RS04955 pilP 1057580..1058164 (+) 585 WP_027221267.1 pilus assembly protein PilP Machinery gene
  CA931_RS04960 pilQ 1058168..1060264 (+) 2097 WP_027221268.1 type IV pilus secretin PilQ Machinery gene
  CA931_RS04965 aroK 1060707..1061234 (+) 528 WP_032828804.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38794.80 Da        Isoelectric Point: 5.0331

>NTDB_id=229205 CA931_RS04940 WP_027221264.1 1055354..1056418(+) (pilM) [Legionella pneumophila subsp. pascullei strain U8W (D-7160)]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEQVCVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDQEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKNSALLDVLIVASRAENVNSRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIIAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSYMSFGKKVNLDAINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229205 CA931_RS04940 WP_027221264.1 1055354..1056418(+) (pilM) [Legionella pneumophila subsp. pascullei strain U8W (D-7160)]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATTAGTTCTTCAGCAGTGAAAATTTTGGAAAT
TTCAGGTTCCGGAGAGCAAGTTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCTCTTGAAGGGAATATGA
TCAAGGACGTCGATGTAGTAGCGAATTGTATCAAAAAATTGGTAGAAAGGTTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATTAGCAAAGTAGTGCAAATTAATGAAGGATTGACTGACCAGGAAATGGAAGAGCTTGT
CGTTATTGAAGCGGATAAGTATATTCCTTACCCAATAGACGAAATAAATCTTGATTTTGAAGTGTTAGGTCATTCTGCCA
AGAATTCAGCGCTACTTGATGTTTTAATCGTGGCCTCAAGGGCAGAAAATGTTAATAGTCGTGTTGAAGCAGCTCATAGA
GCTGGCTTGGAGCCTAAAATCGTTGACGTTGAATCTTATGCAGTAGAACGTTCAGTGCAACAAATTGCTCAAGAATTGCC
AGCAGGTGGACAAGACAAAATTATAGCGATAGTAGATATTGGAGCCAATTACACTCATTTATTTGTTTTGCATGGCATGC
GATTAATCTTTTCAAGAGAAGAAAAATTTGGTGGCATGCAGCTTGTTGAAGCTATTGCAGAACATTATCATATGAATTTA
GAGCAGGCTATTGCCCTAAAGAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGCACTTTGCAGTTTTTTTATTCTACCAGTCAACATGGATTTGTAGACCATATTTTATTAG
CTGGAGGAATGGCAAGGCAACCAGGGTTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTAGCAAATCCG
CTATCCTATATGTCTTTTGGTAAAAAAGTGAATTTGGATGCCATTAATAATGACGCGCCTACACTGATGGTGGCTTGTGG
GTTAGCTCTAAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

96.61

100

0.966
  comM Acinetobacter nosocomialis M2

43.22

100

0.432
  pilM Acinetobacter baumannii D1279779

42.938

100

0.429
  comM Acinetobacter baylyi ADP1

40.96

100

0.41

Multiple sequence alignment