Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CA934_RS07950 Genome accession   NZ_CP021259
Coordinates   1719647..1721371 (+) Length   574 a.a.
NCBI ID   WP_027227022.1    Uniprot ID   A0A2S6EZP9
Organism   Legionella pneumophila strain D-7708     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1714647..1726371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA934_RS07930 - 1714940..1717468 (+) 2529 WP_106225288.1 bifunctional diguanylate cyclase/phosphodiesterase -
  CA934_RS07935 - 1717470..1718039 (-) 570 WP_027221706.1 hypoxanthine-guanine phosphoribosyltransferase -
  CA934_RS07940 - 1718082..1718408 (-) 327 WP_027221707.1 DUF1820 family protein -
  CA934_RS07945 - 1718591..1719334 (-) 744 WP_106225289.1 methyltransferase domain-containing protein -
  CA934_RS07950 pilB 1719647..1721371 (+) 1725 WP_027227022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CA934_RS07955 pilC 1721377..1722597 (+) 1221 WP_027221710.1 type II secretion system F family protein Machinery gene
  CA934_RS07960 pilD 1722643..1723506 (+) 864 WP_106225290.1 A24 family peptidase Machinery gene
  CA934_RS07965 - 1723520..1724725 (-) 1206 WP_192877192.1 cyclic nucleotide-binding domain-containing protein -
  CA934_RS07970 - 1724835..1725428 (-) 594 WP_028378909.1 hypothetical protein -
  CA934_RS07975 - 1725444..1725899 (-) 456 WP_027227026.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62645.11 Da        Isoelectric Point: 5.4362

>NTDB_id=229171 CA934_RS07950 WP_027227022.1 1719647..1721371(+) (pilB) [Legionella pneumophila strain D-7708]
MALATDEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLVQYLVKNKILSAEQIALTAAQNFGVPMMDINCIEVDSI
PASLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSSLIDNLLTAKESQGLSDYVE
DSGDLEGLEISTEDEDQDVDTATSATDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLA
SRITARIKVMSNLDISERRIPQDGGFKMKISKARAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRSNFIK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCDQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=229171 CA934_RS07950 WP_027227022.1 1719647..1721371(+) (pilB) [Legionella pneumophila strain D-7708]
ATGGCTCTAGCTACAGATGAATATAGACTACAGGGAATTGGCCAGCTTCTAGTACTGGAAAAGTTGCTAGATAAAACCAA
AGCAATTGAGTTGCATAAATTGGCCGCAGCAGAAAAGATATCTTTAGTGCAATATCTTGTAAAAAATAAAATACTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCAATGATGGATATTAATTGCATTGAGGTGGACTCTATT
CCTGCGAGTCTGGTTAATGAGAAATTGATTAAACGTCATGCCATGGTCCCCCTATTTAGTCGCGGCACTAACTTGTACCT
TGCAACAGATGATCCCAGTAAGCAAGCTTCATTAAAGGAAATTCAATTTCATACCGGACTGAATACTCATGCTATAGTTG
TAGAAACAGATAAACTGAGTTCTTTGATTGATAACTTATTAACAGCAAAGGAAAGTCAGGGCTTATCGGACTATGTCGAA
GACTCTGGTGATCTGGAAGGTTTGGAAATAAGTACCGAAGATGAAGATCAAGATGTTGATACAGCAACATCTGCAACGGA
TGATGCACCGATTGTAAAGTTCGTTAATAAAATTTTATTGGATGCGATAAGGCAAGGCGCTTCTGATATACATTTTGAGC
CTTATGAACGAGAATACCGGATCAGGTACAGGCAAGATGGTATTTTACATGAGGTAGCTACCCCACCGGCAAGCCTGGCA
TCACGTATTACAGCGCGTATCAAGGTAATGTCTAATTTGGATATATCAGAGAGGCGTATACCCCAGGATGGCGGCTTTAA
AATGAAAATATCCAAAGCACGAGCGATTGACTTCAGAGTGAGCACCTGTCCAACCTCAGCCGGGGAAAAAGTGGTGATGC
GAGTACTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGAGATCCAATTTCATTAAA
GCAATCCAGCGCCCACAGGGAATGATTTTAGTGACAGGCCCAACAGGTAGTGGAAAAACAGTCACCTTATATACGGCATT
GAATATATTAAATACAATTGAAGTGAACATTTCGACTGCAGAGGATCCTGTTGAAATCAAGGTACCTGGCATCAATCAGG
TTAATATTAATCCTAAAGCCGGCCTAACCTTTTCCGGTGCATTACGCTCGTTTCTAAGGCAAGACCCTGATATTATCATG
GTTGGTGAGATACGAGATCTTGAAACAGCTGAAATTGCTGTAAAAGCTGCGCAGACAGGGCATTTGGTTCTTTCTACACT
GCATACCAACAGTGCTGCTGAAACATTAAATCGTTTAGTGAACATGGGGATACCTACTTTTAACATTGCGAGTTCAGTTA
CATTAATTATTGCACAAAGGCTGGCAAGAAAATTATGTAATCAATGTAAGGCTGTGCGCGATGACTTTACCAATCAAGGT
TTGATTGAATTAGGCTTCAAGGAAGCTGATCTGGTTAATCTTAAATTATACAAAGCAGTAGGTTGTGATCAATGCACAAG
TGGCTATCGCGGGCGGGTTGGTCTGTTTGAGGTTTTACCAATGACGAAAGAGCTAGGCCAGTTGATTATGTCAGGTGGTA
ACTCCCTGGATATCTTAAAATTAGCCCAATCTGAAGGTATGCTGACTATCTTTCAATCGGGCATAGAAAAAGTCAAGGAA
GGGATCACAACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6EZP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

96.341

100

0.963
  pilB Acinetobacter baumannii D1279779

51.146

98.78

0.505
  pilB Acinetobacter baylyi ADP1

49.315

100

0.502
  pilB Vibrio cholerae strain A1552

49.723

94.251

0.469
  pilB Vibrio campbellii strain DS40M4

47.397

97.038

0.46
  pilF Neisseria gonorrhoeae MS11

46.466

98.606

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

48.513

93.728

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

92.16

0.368
  pilF Thermus thermophilus HB27

38.806

93.38

0.362

Multiple sequence alignment