Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA934_RS05045 Genome accession   NZ_CP021259
Coordinates   1091680..1092744 (+) Length   354 a.a.
NCBI ID   WP_027221264.1    Uniprot ID   A0AAX2IUM9
Organism   Legionella pneumophila strain D-7708     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1086680..1097744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA934_RS05035 - 1087713..1090097 (-) 2385 WP_027221262.1 penicillin-binding protein 1A -
  CA934_RS05040 - 1090248..1091261 (-) 1014 WP_027221263.1 hypothetical protein -
  CA934_RS05045 pilM 1091680..1092744 (+) 1065 WP_027221264.1 pilus assembly protein PilM Machinery gene
  CA934_RS05050 pilN 1092748..1093296 (+) 549 WP_106224992.1 PilN domain-containing protein Machinery gene
  CA934_RS05055 pilO 1093310..1093909 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA934_RS05060 pilP 1093906..1094490 (+) 585 WP_027221267.1 pilus assembly protein PilP Machinery gene
  CA934_RS05065 pilQ 1094494..1096590 (+) 2097 WP_027221268.1 type IV pilus secretin PilQ Machinery gene
  CA934_RS05070 aroK 1097033..1097560 (+) 528 WP_032828804.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38794.80 Da        Isoelectric Point: 5.0331

>NTDB_id=229163 CA934_RS05045 WP_027221264.1 1091680..1092744(+) (pilM) [Legionella pneumophila strain D-7708]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEQVCVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDQEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKNSALLDVLIVASRAENVNSRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIIAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSYMSFGKKVNLDAINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229163 CA934_RS05045 WP_027221264.1 1091680..1092744(+) (pilM) [Legionella pneumophila strain D-7708]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATTAGTTCTTCAGCAGTGAAAATTTTGGAAAT
TTCAGGTTCCGGAGAGCAAGTTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCTCTTGAAGGGAATATGA
TCAAGGACGTCGATGTAGTAGCGAATTGTATCAAAAAATTGGTAGAAAGGTTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATTAGCAAAGTAGTGCAAATTAATGAAGGATTGACTGACCAGGAAATGGAAGAGCTTGT
CGTTATTGAAGCGGATAAGTATATTCCTTACCCAATAGACGAAATAAATCTTGATTTTGAAGTGTTAGGTCATTCTGCCA
AGAATTCAGCGCTACTTGATGTTTTAATCGTGGCCTCAAGGGCAGAAAATGTTAATAGTCGTGTTGAAGCAGCTCATAGA
GCTGGCTTGGAGCCTAAAATCGTTGACGTTGAATCTTATGCAGTAGAACGTTCAGTGCAACAAATTGCTCAAGAATTGCC
AGCAGGTGGACAAGACAAAATTATAGCGATAGTAGATATTGGAGCCAATTACACTCATTTATTTGTTTTGCATGGCATGC
GATTAATCTTTTCAAGAGAAGAAAAATTTGGTGGCATGCAGCTTGTTGAAGCTATTGCAGAACATTATCATATGAATTTA
GAGCAGGCTATTGCCCTAAAGAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGCACTTTGCAGTTTTTTTATTCTACCAGTCAACATGGATTTGTAGACCATATTTTATTAG
CTGGAGGAATGGCAAGGCAACCAGGGTTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTAGCAAATCCG
CTATCCTATATGTCTTTTGGTAAAAAAGTGAATTTGGATGCCATTAATAATGACGCGCCTACACTGATGGTGGCTTGTGG
GTTAGCTCTAAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

96.61

100

0.966
  comM Acinetobacter nosocomialis M2

43.22

100

0.432
  pilM Acinetobacter baumannii D1279779

42.938

100

0.429
  comM Acinetobacter baylyi ADP1

40.96

100

0.41

Multiple sequence alignment