Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   B0B39_RS04390 Genome accession   NZ_CP020894
Coordinates   992081..993145 (+) Length   354 a.a.
NCBI ID   WP_003632474.1    Uniprot ID   -
Organism   Legionella longbeachae strain F1157CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987081..998145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B0B39_RS04380 (B0B39_15745) - 987837..990203 (-) 2367 WP_085662862.1 penicillin-binding protein 1A -
  B0B39_RS04385 (B0B39_15750) - 990414..991844 (-) 1431 WP_085662863.1 M protein -
  B0B39_RS04390 (B0B39_15755) pilM 992081..993145 (+) 1065 WP_003632474.1 type IV pilus assembly protein PilM Machinery gene
  B0B39_RS04395 (B0B39_15760) pilN 993150..993713 (+) 564 WP_003632473.1 PilN domain-containing protein Machinery gene
  B0B39_RS04400 (B0B39_15765) pilO 993710..994309 (+) 600 WP_012978910.1 type 4a pilus biogenesis protein PilO Machinery gene
  B0B39_RS04405 (B0B39_15770) pilP 994306..994812 (+) 507 WP_003632471.1 pilus assembly protein PilP Machinery gene
  B0B39_RS04410 (B0B39_15775) pilQ 994821..996926 (+) 2106 WP_012978911.1 type IV pilus secretin PilQ Machinery gene
  B0B39_RS04415 (B0B39_15780) aroK 997262..997789 (+) 528 WP_003632469.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39125.36 Da        Isoelectric Point: 5.1674

>NTDB_id=226959 B0B39_RS04390 WP_003632474.1 992081..993145(+) (pilM) [Legionella longbeachae strain F1157CHC]
MLKLLQPQHRSILGIDITSTAVKILEISGKNNELVIENYGREILPPNAMDGNVIKDLDAVSQCVKKIVDRLHTPCKQAAL
AVPDAAIISKVVQINEGLNEEEMEELIATEADKYIPYPIEEINLDFEVLGPHQKNPHLLDVLIVASRAENVNQRVGIAAA
AGLDTVVVDVESYAVERAAQRIALDLPGLGKDKTIAIIDIGACYTHLFVLHRMKLVYSREEKFGDKQLIESIAERYKMTL
EEAALARDKKQLPSNYETEVLEPFKENILLQIKRTLQFFYSTNQDGDVDHILLAGGLAKLPGLVALVKDRLGKTTIIANP
LSYMTPSKKIHLDAINNDAPALMVACGLALRNIE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=226959 B0B39_RS04390 WP_003632474.1 992081..993145(+) (pilM) [Legionella longbeachae strain F1157CHC]
ATGCTCAAATTACTACAACCTCAGCATCGTTCAATTCTCGGAATAGACATTACATCCACGGCAGTCAAAATATTAGAAAT
CTCAGGTAAGAATAATGAGCTTGTTATAGAGAATTACGGACGAGAAATATTACCACCCAATGCAATGGATGGTAATGTTA
TTAAGGATCTTGATGCAGTTTCCCAATGTGTTAAAAAAATAGTTGACCGATTGCATACCCCATGTAAGCAAGCTGCTTTG
GCTGTGCCAGATGCTGCGATAATCAGTAAAGTAGTGCAAATTAATGAAGGCTTAAATGAAGAGGAGATGGAGGAGCTTAT
CGCTACTGAGGCTGATAAATATATTCCTTATCCAATTGAGGAAATTAATCTTGATTTCGAAGTTTTAGGTCCGCATCAGA
AAAATCCGCATCTACTCGATGTACTTATCGTTGCTTCGCGAGCGGAGAATGTGAACCAACGTGTTGGAATAGCGGCGGCT
GCGGGTTTAGATACAGTGGTTGTAGATGTTGAGTCTTACGCTGTGGAGCGAGCAGCACAACGTATTGCTTTAGATTTACC
AGGACTTGGTAAAGATAAAACAATTGCGATTATTGATATTGGAGCATGTTATACCCATTTATTTGTATTGCATAGAATGA
AATTAGTTTATTCCCGAGAAGAAAAATTTGGTGATAAGCAATTAATTGAATCAATTGCTGAACGCTATAAAATGACCTTG
GAAGAGGCAGCTCTTGCCAGGGATAAAAAACAATTACCTTCTAACTATGAAACGGAAGTTTTAGAACCATTTAAAGAAAA
TATCTTGTTACAGATTAAAAGAACCTTGCAGTTTTTTTATTCTACAAACCAAGATGGAGATGTGGATCATATTTTATTAG
CAGGGGGATTAGCCAAATTACCAGGCTTAGTTGCTTTAGTTAAAGACCGTTTAGGAAAAACAACTATTATTGCTAATCCA
CTTTCATATATGACCCCAAGTAAAAAAATCCATTTGGATGCAATAAATAATGATGCACCCGCACTTATGGTAGCTTGTGG
TTTAGCATTGAGAAATATAGAGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

66.667

100

0.667
  pilM Acinetobacter baumannii D1279779

42.655

100

0.427
  comM Acinetobacter nosocomialis M2

42.655

100

0.427
  comM Acinetobacter baylyi ADP1

40.678

100

0.407

Multiple sequence alignment