Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B9K09_RS20080 Genome accession   NZ_CP020892
Coordinates   4373119..4375191 (-) Length   690 a.a.
NCBI ID   WP_087518464.1    Uniprot ID   A0A1Y0KX72
Organism   Pseudomonas sp. M30-35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4368119..4380191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9K09_RS20065 (B9K09_20050) - 4369890..4371428 (-) 1539 WP_087518461.1 SPOR domain-containing protein -
  B9K09_RS20070 (B9K09_20055) aroB 4371441..4372544 (-) 1104 WP_087518462.1 3-dehydroquinate synthase -
  B9K09_RS20075 (B9K09_20060) aroK 4372596..4373114 (-) 519 WP_087518463.1 shikimate kinase AroK -
  B9K09_RS20080 (B9K09_20065) pilQ 4373119..4375191 (-) 2073 WP_087518464.1 type IV pilus secretin PilQ Machinery gene
  B9K09_RS20085 (B9K09_20070) pilP 4375246..4375770 (-) 525 WP_087518465.1 type 4a pilus biogenesis lipoprotein PilP -
  B9K09_RS20090 (B9K09_20075) pilO 4375767..4376390 (-) 624 WP_087518466.1 type 4a pilus biogenesis protein PilO -
  B9K09_RS20095 (B9K09_20080) pilN 4376387..4376959 (-) 573 WP_087518467.1 type 4a pilus biogenesis protein PilN -
  B9K09_RS20100 (B9K09_20085) pilM 4376959..4378023 (-) 1065 WP_087518468.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74410.37 Da        Isoelectric Point: 4.8377

>NTDB_id=226944 B9K09_RS20080 WP_087518464.1 4373119..4375191(-) (pilQ) [Pseudomonas sp. M30-35]
MNCSLTRLSISLLGVLLSPVLMAANLESLDVAAMPGDRVELKMTFDEPVAAPRGYTIEQPARIALDLPGVSNQLGTKNRE
LGVGNARSVTVVEAKDRTRLIINLSNLAPYSTRVEGNNLFVLVGANAGSAVASAAPAPAASTYKKPQTRSYIAQSKAISN
IDFQRGEQGEGNVVITLSDPSVSPDIQEQGGKIRLDFAKTQLPEALRVRLDVKDFATPVQFVSATASANKASIVIEPTGF
YDYLAFQTDNKLTLSIKPLTKDDAEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKASDMAKLFQSVTSADGS
ADDRGSITVDDRTNSIIAYQTQDRLDELRRVVAQLDVAVRQVMIEARIVEANVDYDKSLGVNWRGAQVGNGNFIVGGSGG
DRVGSGGNPATALGNFVDMGVANATSSLGIGFVTDNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGADIPYQE
ASSSGATSTTFVEAALSLEVTPQITPDNRIIMEVKVNKDEPDFANQVNGTPTIRKNEVNAKVLVNDGETIVIGGVFSNEQ
RKGVDKVPFLGDLPYLGRLFRRDIVEDSKSELLIFLTPRIMNTQAVAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=226944 B9K09_RS20080 WP_087518464.1 4373119..4375191(-) (pilQ) [Pseudomonas sp. M30-35]
ATGAATTGCTCCCTTACACGACTAAGTATCTCCTTGTTGGGGGTTCTGCTTTCGCCTGTGCTGATGGCAGCTAATCTAGA
ATCGCTCGACGTAGCTGCGATGCCGGGTGATCGAGTTGAACTGAAAATGACGTTCGACGAGCCAGTTGCTGCGCCGCGTG
GTTACACGATCGAGCAGCCAGCGCGTATTGCGCTTGACCTTCCAGGGGTGTCTAACCAGCTAGGGACTAAAAACCGTGAG
CTGGGTGTAGGTAATGCTCGCAGCGTGACGGTGGTAGAGGCTAAAGATCGCACGCGTCTGATCATCAACCTGTCTAACCT
GGCGCCATACAGCACTCGTGTAGAAGGCAATAACCTGTTTGTCCTGGTCGGTGCCAATGCGGGCTCAGCAGTGGCCTCGG
CAGCGCCTGCGCCAGCGGCTTCTACTTATAAAAAGCCACAAACAAGAAGTTATATTGCTCAGTCGAAGGCTATCAGCAAT
ATCGACTTCCAGCGTGGCGAGCAAGGTGAGGGGAATGTGGTTATCACCTTGTCCGACCCTTCGGTGAGCCCGGATATTCA
AGAGCAGGGCGGGAAAATCCGTTTGGATTTTGCTAAGACCCAGCTGCCTGAAGCGCTCCGTGTGCGCCTGGATGTTAAAG
ACTTCGCAACCCCTGTGCAGTTTGTCAGCGCCACCGCTTCGGCCAACAAAGCCAGTATCGTCATTGAGCCAACCGGCTTT
TATGACTACCTGGCTTTTCAAACTGATAACAAACTGACGCTGAGTATCAAGCCGTTGACCAAAGACGATGCGGAAAAGCG
TAAGACTGAGCGGTTTGCTTACAGTGGTGAAAAGCTCTCGCTGAATTTCCAAGATATCGACGTGCGTTCGGTGCTGCAGC
TGATTGCTGACTTTACCGACCTCAACTTGGTTGCCAGTGACACCGTTGCCGGTAACATCACGCTGCGCTTGCAGAATGTG
CCTTGGGACCAAGCGCTGGATCTGGTGCTGAAAACCAAAGGTTTGGACAAGCGCAAAGTGGGTAACGTTTTGCTGGTCGC
TCCGGCGGATGAAATCGCTGCTCGTGAGCGTCAAGAGTTGGAGTCGCAAAAGCAAATTGCTGAGCTGGCCCCACTGCGCC
GTGAGTTGATTCAGGTTAACTACGCCAAAGCCTCGGATATGGCCAAGCTGTTCCAATCCGTGACCAGTGCTGACGGCAGT
GCTGACGATCGTGGTTCTATTACAGTCGACGACCGCACCAACAGCATCATCGCCTACCAAACACAAGACCGTCTCGATGA
GTTGCGCCGTGTGGTCGCTCAGCTGGATGTCGCTGTGCGTCAGGTGATGATTGAAGCGCGAATTGTTGAGGCGAACGTCG
ACTATGACAAATCGCTGGGTGTTAACTGGCGTGGTGCGCAGGTTGGCAACGGTAACTTCATTGTTGGCGGGAGCGGTGGT
GACCGCGTCGGCTCGGGTGGCAACCCAGCTACTGCGTTAGGTAACTTCGTCGATATGGGGGTGGCTAACGCAACTTCCTC
GCTGGGCATCGGCTTTGTCACCGACAACACCATTCTTGATCTTGAGCTTTCGGCGATGGAAAAAACCGGTAACGGTGAGG
TTGTATCGCAGCCGAAGGTCGTGACCTCGGATAAAGAAACCGCGAAAATCCTCAAGGGTGCGGATATCCCATACCAGGAA
GCTTCCTCAAGCGGTGCGACCTCGACCACTTTCGTTGAGGCTGCTCTGTCGCTCGAAGTGACTCCGCAGATCACACCTGA
CAACCGGATTATCATGGAAGTCAAAGTGAACAAGGATGAGCCGGACTTCGCCAACCAGGTAAACGGTACGCCAACCATTC
GTAAGAATGAGGTCAATGCCAAGGTCTTGGTTAACGATGGCGAAACCATTGTGATTGGTGGTGTGTTCTCCAACGAGCAG
CGTAAAGGTGTTGATAAGGTACCTTTCTTGGGCGATCTGCCTTATCTGGGCCGCCTGTTCCGTCGTGATATCGTTGAGGA
CTCAAAATCTGAGTTGCTGATCTTCCTCACCCCGCGCATCATGAACACCCAAGCTGTCGCGGTGAGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(41-121)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0KX72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.21

100

0.778
  pilQ Acinetobacter baumannii strain A118

41.691

100

0.422
  pilQ Acinetobacter baumannii D1279779

41.691

100

0.422
  comQ Acinetobacter baylyi ADP1

41.8

99.855

0.417
  pilQ Legionella pneumophila strain Lp02

36.603

98.986

0.362

Multiple sequence alignment