Detailed information NTG0226945

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B9K09_RS20100
• Genome accession: NZ_CP020892
• Coordinates: 4376959..4378023 (-)
• Length: 354 a.a.
• NCBI ID: WP_087518468.1
• Uniprot ID: -
• Organism: Pseudomonas sp. M30-35
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4371959..4383023

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B9K09_RS20075 (B9K09_20060)	aroK	4372596..4373114 (-)	519	WP_087518463.1	shikimate kinase AroK	-
B9K09_RS20080 (B9K09_20065)	pilQ	4373119..4375191 (-)	2073	WP_087518464.1	type IV pilus secretin PilQ	Machinery gene
B9K09_RS20085 (B9K09_20070)	pilP	4375246..4375770 (-)	525	WP_087518465.1	type 4a pilus biogenesis lipoprotein PilP	-
B9K09_RS20090 (B9K09_20075)	pilO	4375767..4376390 (-)	624	WP_087518466.1	type 4a pilus biogenesis protein PilO	-
B9K09_RS20095 (B9K09_20080)	pilN	4376387..4376959 (-)	573	WP_087518467.1	type 4a pilus biogenesis protein PilN	-
B9K09_RS20100 (B9K09_20085)	pilM	4376959..4378023 (-)	1065	WP_087518468.1	pilus assembly protein PilM	Machinery gene
B9K09_RS20105 (B9K09_20090)	-	4378205..4380685 (+)	2481	WP_087518469.1	penicillin-binding protein 1A	-
B9K09_RS20110 (B9K09_20095)	-	4380774..4382042 (-)	1269	WP_087518470.1	malic enzyme-like NAD(P)-binding protein	-
B9K09_RS20115 (B9K09_20100)	-	4382214..4383014 (-)	801	WP_087518471.1	thermonuclease family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37768.20 Da
• Isoelectric Point: 4.3976

>NTDB_id=226945 B9K09_RS20100 WP_087518468.1 4376959..4378023(-) (pilM) [Pseudomonas sp. M30-35]
MLGLFNKKANTLLGIDISSTSVKLLELSCSGGRYKVEAYAVEPLPPNAVVEKNIAEMEGVGQALTRVLSKAKTGVKTVAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGLSARNAERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFALLAEQLGSGADDLTVAVVDIGATMTTLSVLHNGKTIYTREQMFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKQAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=226945 B9K09_RS20100 WP_087518468.1 4376959..4378023(-) (pilM) [Pseudomonas sp. M30-35]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGTTCGACTTCGGTAAAGCTCCTCGAGTT
GAGTTGCTCAGGAGGCCGCTACAAAGTAGAGGCCTACGCTGTTGAGCCGCTCCCACCTAATGCTGTAGTTGAGAAAAACA
TCGCCGAGATGGAAGGGGTCGGCCAAGCGCTCACGCGTGTGCTTTCCAAAGCCAAGACAGGCGTTAAAACTGTTGCTGTT
GCAGTGGCAGGGTCTGCGGTGATTACCAAGACCATCGAAATGGAAGCCGGGCTTTCTGATGATGATCTTGAGAATCAATT
GAAGATCGAAGCGGATCAATACATTCCTTACCCGCTCGAAGAAGTTGCGATTGACTTCGAGGTGCAGGGATTGTCTGCGC
GTAATGCCGAACGTGTTGAGGTATTACTCGCGGCGTGCCGCAAAGAGAATGTCGAGGTTCGTGAGGCCGCGTTGGCGCTT
GCAGGCCTGACCGCCAAAGTGGTTGATGTTGAGGCGTATGCGCTCGAACGCTCTTTTGCGTTGCTCGCAGAACAGCTCGG
TTCTGGCGCTGATGACCTGACCGTAGCGGTTGTTGATATCGGCGCAACGATGACCACGTTGAGCGTTCTGCATAATGGCA
AGACTATCTATACCCGCGAGCAAATGTTCGGCGGCAAGCAACTGACTGAAGAGATTCAGCGCCGTTATGGCTTATCTGTT
GAAGAAGCCGGTCTTGCGAAAAAACAAGGCGGTCTTCCAGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAACAAGC
GGTTGTGCAGCAGGTTTCTCGCTCGCTGCAATTCTTCTTCGCGGCGGGTCAGTACAACGACGTTGATTACATCCTGTTGG
CCGGAGGTACGGCTTCCATTCCTGATCTCGATCGCTTGATTCAGCAGAAAATCGGCACCCAGACATTGGTCGCCAATCCG
TTCGCTGATATGGCGTTAAGTAGCAAGGTCAATGCTGGGGCGCTCGCGAGTGATGCACCTGCACTGATGATTGCTTGTGG
TCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.062	100	0.571
comM	Acinetobacter nosocomialis M2	57.062	100	0.571
comM	Acinetobacter baylyi ADP1	53.955	100	0.54
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466