Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B6N58_RS07975 Genome accession   NZ_CP020614
Coordinates   1769740..1771446 (+) Length   568 a.a.
NCBI ID   WP_045099173.1    Uniprot ID   A0A098GFR0
Organism   Legionella micdadei strain NZ2015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1764740..1776446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6N58_RS07960 (B6N58_07960) - 1767769..1768329 (-) 561 WP_045099176.1 hypoxanthine-guanine phosphoribosyltransferase -
  B6N58_RS07965 (B6N58_07965) - 1768356..1768682 (-) 327 WP_045099175.1 DUF1820 family protein -
  B6N58_RS07970 (B6N58_07970) - 1768795..1769574 (-) 780 WP_045099174.1 methyltransferase domain-containing protein -
  B6N58_RS07975 (B6N58_07975) pilB 1769740..1771446 (+) 1707 WP_045099173.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B6N58_RS07980 (B6N58_07980) pilC 1771449..1772657 (+) 1209 WP_349267104.1 type II secretion system F family protein Machinery gene
  B6N58_RS07985 (B6N58_07985) - 1772661..1773515 (+) 855 WP_045099172.1 A24 family peptidase -
  B6N58_RS07990 (B6N58_07990) - 1773561..1774703 (+) 1143 WP_045099171.1 hypothetical protein -
  B6N58_RS07995 (B6N58_07995) - 1775043..1775627 (-) 585 WP_045099170.1 hypothetical protein -
  B6N58_RS08000 (B6N58_08000) - 1775697..1776143 (-) 447 WP_045099169.1 hypothetical protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63900.38 Da        Isoelectric Point: 7.0140

>NTDB_id=224962 B6N58_RS07975 WP_045099173.1 1769740..1771446(+) (pilB) [Legionella micdadei strain NZ2015]
MEMTTITNHRLQGIAQLLTANNLISKSKIFEYQTLALANQQTLLQYLATNDLIEPPVIAQIMANYFDLPFMDLDNIDFDS
IPIHLISGQLTRRHAVLPIIIRAENLILATDDPSQQSVFKEIHFQTGLPTKVVVVETDKLRKLIDRVMNKKENQHLLDYL
DNSVQLENITPVNTDFAFTTHNNEAPVVNFVNQILISAINNGASDVHFEPYEQAYRIRYRQDGLLTEVTDPPRHLSNQIS
TRIKVLANLDISERRMPQDGQFQIKLSSHHTVDCRVSTCPTIYGEKLVVRILDADLSYFHIDSLGLNHLQKEHFLAALRK
PQGLILATGPTGSGKTLSLYTALNLMNSIERNISTIEDPVEIKIPGINQVNINPKSGLTFSTALRSFLRQDPDIIMIGEI
RDTETAEIAIKAAQTGHLVLSTLHANSTSETLTRLINLGIPAFNIANSCILIIAQRLARRLCDRCKTIRNDLSHRDLFGL
GFHQNEKANLYQARACEHCRNGYRGRLGLFEVMPISKLIRHQILCGANSVEIQKLAQTEGMLTVYQSGLEKVRQGLTSLE
ELNRVIAD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=224962 B6N58_RS07975 WP_045099173.1 1769740..1771446(+) (pilB) [Legionella micdadei strain NZ2015]
ATGGAAATGACAACAATAACAAACCATCGCTTACAAGGAATTGCTCAATTACTTACAGCAAATAATCTTATTTCTAAAAG
CAAAATTTTCGAATATCAAACCCTTGCTCTCGCTAACCAACAAACTCTCCTACAGTATTTAGCCACAAACGATTTGATTG
AACCCCCGGTTATTGCACAAATCATGGCTAATTATTTTGATTTACCTTTTATGGATCTTGATAATATTGACTTCGACTCC
ATTCCTATACACCTAATCAGTGGACAATTAACACGCCGTCATGCTGTTTTACCCATTATCATTCGCGCGGAGAATTTGAT
TTTAGCGACAGATGATCCAAGTCAGCAGTCTGTTTTCAAAGAAATTCACTTTCAAACTGGCTTACCCACTAAAGTTGTTG
TTGTTGAAACAGATAAGCTTCGGAAACTCATTGACAGGGTGATGAATAAAAAAGAAAACCAACACTTACTCGATTATTTA
GATAATTCAGTTCAATTGGAAAACATCACTCCTGTGAACACAGATTTTGCTTTCACAACACACAATAACGAGGCTCCCGT
TGTCAACTTCGTTAATCAAATCTTGATTTCGGCAATCAATAACGGCGCATCAGATGTTCATTTTGAACCTTATGAACAAG
CATATCGTATTCGTTATCGTCAAGACGGATTATTAACCGAAGTAACCGACCCGCCTAGACATTTATCAAACCAAATAAGC
ACTCGCATTAAGGTGCTGGCAAATCTAGATATCTCCGAGCGTCGTATGCCCCAAGATGGCCAATTTCAAATTAAGCTATC
TTCTCACCATACAGTTGATTGCAGAGTAAGCACTTGTCCTACTATTTATGGTGAAAAACTAGTAGTACGTATTCTTGACG
CCGATTTAAGCTATTTTCATATTGATTCACTAGGATTAAATCACTTACAGAAAGAACATTTCCTTGCTGCTTTGAGGAAA
CCACAAGGGTTGATTTTAGCTACAGGCCCAACAGGTAGTGGTAAAACCTTATCGCTTTATACCGCTCTCAACCTCATGAA
CAGTATTGAACGAAATATCTCCACTATCGAAGATCCGGTTGAAATTAAAATACCAGGTATCAACCAGGTTAATATTAACC
CTAAATCGGGTTTAACTTTCTCGACTGCCTTACGTTCATTTCTGCGGCAAGATCCTGATATCATCATGATTGGCGAGATT
CGGGATACAGAAACTGCCGAAATTGCAATTAAAGCCGCACAAACTGGCCATCTTGTTCTATCTACCTTGCATGCGAATAG
TACTTCAGAAACACTCACTCGACTCATTAATCTTGGCATTCCGGCCTTTAATATTGCAAATTCCTGCATCTTGATTATTG
CGCAGCGGTTAGCCAGACGTTTATGCGATCGGTGTAAAACCATTCGCAATGATTTATCTCATAGGGATTTGTTTGGGCTT
GGCTTTCATCAGAACGAGAAAGCTAATCTTTACCAAGCCCGAGCTTGTGAGCATTGTAGGAATGGTTATCGTGGCCGATT
AGGTTTGTTTGAGGTGATGCCAATCTCCAAATTAATCAGGCACCAGATTTTATGCGGAGCAAATTCTGTTGAGATTCAAA
AATTAGCCCAAACAGAAGGAATGCTCACAGTTTATCAATCAGGTCTTGAAAAAGTCAGGCAAGGATTAACCTCGCTGGAA
GAATTAAACCGCGTCATTGCTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098GFR0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.496

99.824

0.574
  pilB Acinetobacter baumannii D1279779

46.208

99.824

0.461
  pilB Acinetobacter baylyi ADP1

47.015

94.366

0.444
  pilB Vibrio cholerae strain A1552

47.573

90.669

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

46.023

92.958

0.428
  pilB Vibrio campbellii strain DS40M4

46.449

91.725

0.426
  pilF Neisseria gonorrhoeae MS11

42.5

98.592

0.419
  pilB/pilB1 Synechocystis sp. PCC 6803

36.538

100

0.368

Multiple sequence alignment