Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A6J88_RS19805 Genome accession   NZ_CP020452
Coordinates   1124364..1126043 (+) Length   559 a.a.
NCBI ID   WP_060974402.1    Uniprot ID   -
Organism   Neisseria mucosa strain FDAARGOS_260     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1119364..1131043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J88_RS19780 (A6J88_05695) waaA 1119507..1120769 (-) 1263 WP_080613749.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  A6J88_RS19785 (A6J88_05700) - 1121593..1122339 (+) 747 WP_240594061.1 hypothetical protein -
  A6J88_RS19795 (A6J88_05710) - 1123202..1123549 (+) 348 WP_240594077.1 YadA C-terminal domain-containing protein -
  A6J88_RS19800 (A6J88_05715) - 1123617..1124084 (+) 468 WP_049226372.1 outer membrane protein assembly factor BamE -
  A6J88_RS19805 (A6J88_05720) pilF 1124364..1126043 (+) 1680 WP_060974402.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A6J88_RS19810 (A6J88_05725) pilG 1126115..1127356 (+) 1242 WP_060974403.1 type II secretion system F family protein Machinery gene
  A6J88_RS19815 (A6J88_05730) pilD 1127358..1128221 (+) 864 WP_080613751.1 prepilin peptidase Machinery gene
  A6J88_RS19820 (A6J88_05735) coaE 1128223..1128852 (+) 630 WP_080613752.1 dephospho-CoA kinase -
  A6J88_RS19825 (A6J88_05740) yacG 1128907..1129101 (+) 195 WP_045074220.1 DNA gyrase inhibitor YacG -
  A6J88_RS19830 (A6J88_05745) ribF 1129203..1130132 (-) 930 WP_080613753.1 bifunctional riboflavin kinase/FAD synthetase -
  A6J88_RS19835 (A6J88_05750) - 1130237..1130629 (-) 393 WP_080613754.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62406.97 Da        Isoelectric Point: 6.3585

>NTDB_id=223301 A6J88_RS19805 WP_060974402.1 1124364..1126043(+) (pilF) [Neisseria mucosa strain FDAARGOS_260]
MSIGLLRVLVQSQTINNTQAEHYNNILKTGQEILPMLFADKIISPRSLGELVARVFSYPLLDLHYYPRNNIVMDVLTEEQ
MVQNRCVPIFRRGRKVYLAVSDPTQIQSFQKIAFASGVTVDLVVVPDDQLSSLLEWLGQRSTTILKEISEEQEAAQPSQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKREVERPPVPALKKAGFTDEDLAKDWKL
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRAGLLKAMQGLTSLEEVIAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=223301 A6J88_RS19805 WP_060974402.1 1124364..1126043(+) (pilF) [Neisseria mucosa strain FDAARGOS_260]
ATGAGTATCGGATTATTACGAGTTTTGGTTCAAAGCCAGACAATCAACAATACCCAGGCAGAGCATTACAATAATATATT
GAAAACGGGGCAGGAAATCTTGCCCATGCTTTTTGCGGATAAGATTATCAGCCCAAGGTCTTTGGGCGAGTTGGTTGCGC
GAGTTTTTAGTTATCCGCTATTGGATTTGCATTATTATCCACGTAACAATATTGTTATGGATGTCTTAACTGAGGAGCAG
ATGGTTCAAAATCGCTGCGTTCCGATTTTTCGTCGCGGCCGAAAGGTATATTTGGCGGTTTCAGATCCTACTCAAATCCA
AAGTTTTCAAAAAATAGCTTTTGCTTCGGGAGTGACGGTTGATTTAGTAGTTGTCCCGGATGATCAGTTGAGTTCTTTGC
TGGAGTGGTTAGGACAACGTTCTACCACCATCTTAAAAGAAATTAGTGAAGAACAAGAAGCTGCCCAGCCCTCTCAGGCG
CTTTATATAGACAACGAAGAGGCTGAAGATGGGCCTATTCCACGTTTTATTCACAAGACCTTATCAGATGCGCTAAATGC
AGGCGCATCCGATATACATTTCGAATTTTATGAACAAATGGCTCGTGTCCGTTTTCGTGTAGATGGACAGCTAAGGGAAG
TGGTTCAACCCCCTGTTGCTGTACGCGGACAGCTGGCTTCACGCATCAAAGTAATGGCGCGTTTGGACATTTCGGAAAAA
CGCGTACCGCAAGATGGTCGTATTCAGATTGCTTTCCATAAACACGGTCGCCCCATTGATTTTCGTGTAAGTACGTTACC
TACGCTTTTTGGCGAAAAAGTGGTAATGCGTATTCTGAATTCTGATGCAGCAACTTTAAATATTGACCAACTGGGATTCG
AACCCTTCCAAAAAGAAATGCTGCTTGAGGCCATTCATCGGCCTTATGGCATGGTATTGGTAACAGGTCCGACAGGCTCC
GGTAAAACCGTATCACTTTATACTTGCTTGAATATTTTAAATACAGAAGACGTCAATATTTCCACTGCAGAAGACCCGGC
CGAGATTAATTTACCCGGCATCAACCAAGTCAACGTCAATGACAAACAAGGTTTGACTTTTGCTGCGGCGTTGAAATCTT
TCTTGCGACAAGACCCTGACATTATCATGGTAGGTGAGATTCGGGACTTGGAAACCGCGGATATTGCCATCAAAGCGGCA
CAAACCGGACATATGGTGTTTTCAACGTTGCATACCAATAACGCGCCTGCGACCCTATCCCGTATGTTGAACATGGGGGT
CGCTCCATTCAACATTGCCAGTTCCGTCAGCTTAATTATGGCGCAACGACTATTGCGTCGTCTGTGTTCGAGCTGTAAGC
GTGAGGTTGAGCGTCCTCCAGTTCCCGCATTAAAAAAAGCAGGATTTACAGATGAAGATTTAGCAAAGGATTGGAAACTC
TACCGTCCTGTCGGCTGCGATAGTTGCCGTGGTAAAGGCTTCAAAGGCCGTGTCGGCGTCTACGAAGTGATGCCGATTAC
CGAGGAAATGCAACGTGTCATTATGAATAATGGTACGGAAGTTGATATTATGAACATGGCCTATAAAGAAGGCATGGTCG
ATTTACGCCGCGCAGGTTTGTTAAAAGCTATGCAAGGTTTGACTTCATTAGAAGAAGTTATTGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

80.322

100

0.803
  pilB Acinetobacter baylyi ADP1

51.06

100

0.517
  pilB Acinetobacter baumannii D1279779

51.259

99.463

0.51
  pilB Legionella pneumophila strain ERS1305867

48.214

100

0.483
  pilB Vibrio cholerae strain A1552

45.907

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

44.858

100

0.453
  pilB Vibrio campbellii strain DS40M4

43.262

100

0.436
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.575

92.665

0.367

Multiple sequence alignment