Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A6J53_RS01985 Genome accession   NZ_CP020401
Coordinates   327258..328934 (-) Length   558 a.a.
NCBI ID   WP_002231695.1    Uniprot ID   A0A9K2KN84
Organism   Neisseria meningitidis strain FDAARGOS_214     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322258..333934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J53_RS01965 (A6J53_01930) pilG 324171..325403 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  A6J53_RS01970 (A6J53_01935) pilD 325477..326337 (+) 861 WP_002256367.1 A24 family peptidase Machinery gene
  A6J53_RS01975 (A6J53_01940) coaE 326339..326971 (+) 633 WP_002236672.1 dephospho-CoA kinase -
  A6J53_RS01980 (A6J53_01945) - 326964..327173 (+) 210 WP_002224872.1 DNA gyrase inhibitor YacG -
  A6J53_RS01985 (A6J53_01950) pilF 327258..328934 (-) 1677 WP_002231695.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A6J53_RS02005 (A6J53_01960) - 329426..329872 (-) 447 WP_002216397.1 DUF441 domain-containing protein -
  A6J53_RS02010 (A6J53_01965) ispB 329877..330851 (-) 975 WP_002256366.1 octaprenyl diphosphate synthase -
  A6J53_RS02020 (A6J53_01975) rplU 331075..331383 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  A6J53_RS02025 (A6J53_01980) rpmA 331408..331680 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  A6J53_RS02030 (A6J53_01985) ubiM 332069..333253 (+) 1185 WP_002233502.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  A6J53_RS02040 (A6J53_01990) rpmG 333524..333679 (-) 156 WP_002212306.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61979.12 Da        Isoelectric Point: 5.4180

>NTDB_id=222151 A6J53_RS01985 WP_002231695.1 327258..328934(-) (pilF) [Neisseria meningitidis strain FDAARGOS_214]
MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=222151 A6J53_RS01985 WP_002231695.1 327258..328934(-) (pilF) [Neisseria meningitidis strain FDAARGOS_214]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAAGGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATTGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.265

100

0.511
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.439

95.878

0.378
  pilF Thermus thermophilus HB27

37.132

97.491

0.362

Multiple sequence alignment