Detailed information NTG0222150

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A6J53_RS01970
• Genome accession: NZ_CP020401
• Coordinates: 325477..326337 (+)
• Length: 286 a.a.
• NCBI ID: WP_002256367.1
• Uniprot ID: A0A378VS00
• Organism: Neisseria meningitidis strain FDAARGOS_214
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 320477..331337

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A6J53_RS01945 (A6J53_01915)	dapD	320987..321808 (-)	822	WP_002224875.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
A6J53_RS01950 (A6J53_01920)	-	321818..322048 (-)	231	WP_002212338.1	hypothetical protein	-
A6J53_RS01955 (A6J53_01925)	pgi	322018..323661 (-)	1644	WP_002256368.1	glucose-6-phosphate isomerase	-
A6J53_RS01965 (A6J53_01930)	pilG	324171..325403 (+)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
A6J53_RS01970 (A6J53_01935)	pilD	325477..326337 (+)	861	WP_002256367.1	A24 family peptidase	Machinery gene
A6J53_RS01975 (A6J53_01940)	coaE	326339..326971 (+)	633	WP_002236672.1	dephospho-CoA kinase	-
A6J53_RS01980 (A6J53_01945)	-	326964..327173 (+)	210	WP_002224872.1	DNA gyrase inhibitor YacG	-
A6J53_RS01985 (A6J53_01950)	pilF	327258..328934 (-)	1677	WP_002231695.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A6J53_RS02005 (A6J53_01960)	-	329426..329872 (-)	447	WP_002216397.1	DUF441 domain-containing protein	-
A6J53_RS02010 (A6J53_01965)	ispB	329877..330851 (-)	975	WP_002256366.1	octaprenyl diphosphate synthase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31363.39 Da
• Isoelectric Point: 8.2213

>NTDB_id=222150 A6J53_RS01970 WP_002256367.1 325477..326337(+) (pilD) [Neisseria meningitidis strain FDAARGOS_214]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLL
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGETGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=222150 A6J53_RS01970 WP_002256367.1 325477..326337(+) (pilD) [Neisseria meningitidis strain FDAARGOS_214]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
TGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGCCAAACCAAAATCAGCATACGCTATCC
CTTAATCGAACTGCTGACCGGCGTATTGTTCGGGCTGGTTGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCTC
TTGATTTGGCTGGGACTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

3D structure

• AlphaFold DB: A0A378VS00

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	96.14	99.65	0.958
pilD	Vibrio cholerae strain A1552	44.097	100	0.444
pilD	Acinetobacter baumannii D1279779	46.591	92.308	0.43
pilD	Acinetobacter nosocomialis M2	46.591	92.308	0.43
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43