Detailed information NTG0220098

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OTEC02_RS14465
• Genome accession: NZ_CP020015
• Coordinates: 3053138..3054256 (+)
• Length: 372 a.a.
• NCBI ID: WP_005803129.1
• Uniprot ID: A0A009MM04
• Organism: Acinetobacter lactucae strain OTEC-02
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3048138..3059256

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OTEC02_RS14455 (OTEC02_14455)	-	3051261..3051953 (-)	693	WP_081175031.1	YggS family pyridoxal phosphate-dependent enzyme	-
OTEC02_RS14460 (OTEC02_14460)	pilT	3052080..3053117 (+)	1038	WP_081175033.1	type IV pilus twitching motility protein PilT	Machinery gene
OTEC02_RS14465 (OTEC02_14465)	pilU	3053138..3054256 (+)	1119	WP_005803129.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OTEC02_RS14470 (OTEC02_14470)	fur	3054326..3054763 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
OTEC02_RS14475 (OTEC02_14475)	-	3054875..3055273 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
OTEC02_RS14480 (OTEC02_14480)	-	3055338..3055652 (-)	315	WP_016144536.1	RnfH family protein	-
OTEC02_RS14485 (OTEC02_14485)	-	3055652..3056719 (-)	1068	WP_155859225.1	hypothetical protein	-
OTEC02_RS14495 (OTEC02_14495)	-	3056873..3057328 (-)	456	WP_002121698.1	bacteriohemerythrin	-
OTEC02_RS14500 (OTEC02_14500)	-	3057580..3058428 (-)	849	WP_016144533.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41903.09 Da
• Isoelectric Point: 6.6403

>NTDB_id=220098 OTEC02_RS14465 WP_005803129.1 3053138..3054256(+) (pilU) [Acinetobacter lactucae strain OTEC-02]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=220098 OTEC02_RS14465 WP_005803129.1 3053138..3054256(+) (pilU) [Acinetobacter lactucae strain OTEC-02]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGAGATAAATCTGCC
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCGGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTACAACTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGTATCATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACCTCATTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATTATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTCATCTTGATTGGTGAGATTCGTTCTCGTGAAGTAATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAAGTGATCGTCACAGCCAGCTCTACATGGACCTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACACCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCGCTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGATTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A009MM04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363