Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OTEC02_RS01520 Genome accession   NZ_CP020015
Coordinates   315084..315863 (+) Length   259 a.a.
NCBI ID   WP_081172659.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain OTEC-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310084..320863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTEC02_RS01505 (OTEC02_01505) ponA 310665..313226 (-) 2562 WP_155859195.1 penicillin-binding protein PBP1a -
  OTEC02_RS01510 (OTEC02_01510) comM 313388..314446 (+) 1059 WP_057104815.1 pilus assembly protein PilM Machinery gene
  OTEC02_RS01515 (OTEC02_01515) pilN 314446..315087 (+) 642 WP_031949513.1 PilN domain-containing protein Machinery gene
  OTEC02_RS01520 (OTEC02_01520) pilO 315084..315863 (+) 780 WP_081172659.1 type 4a pilus biogenesis protein PilO Machinery gene
  OTEC02_RS01525 (OTEC02_01525) pilP 315875..316402 (+) 528 WP_081172660.1 pilus assembly protein PilP Machinery gene
  OTEC02_RS01530 (OTEC02_01530) pilQ 316465..318630 (+) 2166 WP_081172661.1 type IV pilus secretin PilQ Machinery gene
  OTEC02_RS01535 (OTEC02_01535) aroK 318642..319211 (+) 570 WP_081172662.1 shikimate kinase AroK -
  OTEC02_RS01540 (OTEC02_01540) aroB 319240..320322 (+) 1083 WP_061875201.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29288.25 Da        Isoelectric Point: 6.0348

>NTDB_id=220073 OTEC02_RS01520 WP_081172659.1 315084..315863(+) (pilO) [Acinetobacter lactucae strain OTEC-02]
MSPHELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFAAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDSKSDIPVLNYSIKAKTYRYMGAGENQNNNDPQGVNGETQA
NASDGHNVNIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=220073 OTEC02_RS01520 WP_081172659.1 315084..315863(+) (pilO) [Acinetobacter lactucae strain OTEC-02]
ATGAGTCCTCATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCCTCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAATACACTCGATATGAACAGCTATGGCAGTTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGCTATTTTGCTGCTATCCAGCCAAAGCTCCAAGCAATTGATAATGCTCAGGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGTAACTTGCAGCAATATCAGCTTCAACTTCAAGAAAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGAGTGAATTCTGGTCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAGGAAATCTTTATTGAACAGCCG
ATTACGATTGAGGCAACAGGCGATTATCACGCTTTCGGTGCTTTCGTGAGCAGTATTGCGGCATTACCGCGTATTGTGAC
GATGCATGATTTTGTTGTTGAAGCTGCTCCTGTTAAAGATAGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGCGCTGGGGAAAACCAAAATAATAATGACCCGCAAGGTGTAAATGGTGAGACGCAAGCC
AACGCCTCCGATGGTCATAATGTAAATATTCCTTCTATAAAGAAATCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.286

86.486

0.772
  comO Acinetobacter baylyi ADP1

69.547

93.822

0.653

Multiple sequence alignment