Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OTEC02_RS01515 Genome accession   NZ_CP020015
Coordinates   314446..315087 (+) Length   213 a.a.
NCBI ID   WP_031949513.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain OTEC-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 309446..320087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTEC02_RS01495 (OTEC02_01495) - 309490..309822 (+) 333 WP_016143665.1 hypothetical protein -
  OTEC02_RS01500 (OTEC02_01500) - 309825..310649 (+) 825 WP_081172658.1 putative RNA methyltransferase -
  OTEC02_RS01505 (OTEC02_01505) ponA 310665..313226 (-) 2562 WP_155859195.1 penicillin-binding protein PBP1a -
  OTEC02_RS01510 (OTEC02_01510) comM 313388..314446 (+) 1059 WP_057104815.1 pilus assembly protein PilM Machinery gene
  OTEC02_RS01515 (OTEC02_01515) pilN 314446..315087 (+) 642 WP_031949513.1 PilN domain-containing protein Machinery gene
  OTEC02_RS01520 (OTEC02_01520) pilO 315084..315863 (+) 780 WP_081172659.1 type 4a pilus biogenesis protein PilO Machinery gene
  OTEC02_RS01525 (OTEC02_01525) pilP 315875..316402 (+) 528 WP_081172660.1 pilus assembly protein PilP Machinery gene
  OTEC02_RS01530 (OTEC02_01530) pilQ 316465..318630 (+) 2166 WP_081172661.1 type IV pilus secretin PilQ Machinery gene
  OTEC02_RS01535 (OTEC02_01535) aroK 318642..319211 (+) 570 WP_081172662.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23961.42 Da        Isoelectric Point: 5.4186

>NTDB_id=220072 OTEC02_RS01515 WP_031949513.1 314446..315087(+) (pilN) [Acinetobacter lactucae strain OTEC-02]
MATINLLPWRDGLREQRKKQFIILCFGVVVLGITTVFSGWFYLNQKLSDQEQANQLIISTNQNLDQQLKTLNGLQEQRDA
IIERMKLIQGLQSQRPVVVRLVDELVRVTPAAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
TEEKKDKAVSSLLPRVEDSYGSFVVTVDVGEMGVTTTDDTANTPVGTDKEVAK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=220072 OTEC02_RS01515 WP_031949513.1 314446..315087(+) (pilN) [Acinetobacter lactucae strain OTEC-02]
ATGGCAACAATTAATTTACTCCCTTGGCGTGATGGGCTTCGGGAGCAGCGCAAAAAACAATTTATTATTTTATGTTTTGG
GGTCGTTGTTTTGGGGATAACCACTGTATTTTCTGGGTGGTTTTATTTGAATCAAAAACTCAGTGATCAAGAACAAGCGA
ATCAACTCATTATCAGTACAAACCAGAATCTAGATCAACAGCTTAAAACCTTAAATGGCTTGCAAGAACAGCGTGACGCA
ATTATTGAGCGTATGAAGCTCATTCAAGGCTTACAAAGTCAGCGTCCAGTCGTTGTACGTTTGGTGGATGAGTTGGTACG
CGTGACTCCAGCAGCCATGTATTTGACTAAATTCAGCCGAACTGGAGATAAATTTACCATTGAAGGTAAAGCGGAAAGTC
CAAATACAGTTGCAGAGTTATTACGTAATCTGGAAGCATCACCATGGTATCGCAACGCTTTTATGAATTCTTTCTTAGCG
ACTGAAGAGAAAAAAGATAAGGCTGTAAGTTCTTTGCTTCCTCGAGTAGAAGATAGCTATGGCAGTTTTGTTGTTACTGT
TGATGTAGGGGAAATGGGAGTGACTACAACAGATGATACCGCCAATACGCCTGTAGGTACGGATAAGGAGGTAGCAAAAT
GA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

88.732

100

0.887
  comN Acinetobacter baylyi ADP1

71.707

96.244

0.69

Multiple sequence alignment