Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PspR84_RS24665 Genome accession   NZ_CP019426
Coordinates   5587173..5588873 (+) Length   566 a.a.
NCBI ID   WP_160059458.1    Uniprot ID   A0A7Z2KXK2
Organism   Pseudomonas sp. R84     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5582173..5593873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR84_RS24645 (PspR84_24895) - 5582352..5585303 (+) 2952 WP_160059455.1 DUF748 domain-containing protein -
  PspR84_RS24650 (PspR84_24900) - 5585303..5585599 (+) 297 WP_160059456.1 DUF2845 domain-containing protein -
  PspR84_RS24655 (PspR84_24905) - 5585871..5586227 (-) 357 WP_047600285.1 BON domain-containing protein -
  PspR84_RS24660 (PspR84_24910) - 5586525..5586947 (-) 423 WP_160059457.1 pilin -
  PspR84_RS24665 (PspR84_24915) pilB 5587173..5588873 (+) 1701 WP_160059458.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PspR84_RS24670 (PspR84_24920) pilC 5588876..5590093 (+) 1218 WP_160059459.1 type II secretion system F family protein Machinery gene
  PspR84_RS24675 (PspR84_24925) pilD 5590095..5590967 (+) 873 WP_160059460.1 A24 family peptidase Machinery gene
  PspR84_RS24680 (PspR84_24930) coaE 5590964..5591587 (+) 624 WP_160059461.1 dephospho-CoA kinase -
  PspR84_RS24685 (PspR84_24935) yacG 5591584..5591784 (+) 201 WP_160059462.1 DNA gyrase inhibitor YacG -
  PspR84_RS24690 (PspR84_24940) - 5591790..5592005 (-) 216 WP_038369048.1 hypothetical protein -
  PspR84_RS24695 (PspR84_24945) - 5592071..5592760 (-) 690 WP_160059463.1 energy-coupling factor ABC transporter permease -
  PspR84_RS24700 (PspR84_24950) - 5592933..5593559 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62680.66 Da        Isoelectric Point: 5.9192

>NTDB_id=215646 PspR84_RS24665 WP_160059458.1 5587173..5588873(+) (pilB) [Pseudomonas sp. R84]
MNDIALSGLAKQLVQAELLTEKGAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
LDVESIDDSKQEAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPVQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=215646 PspR84_RS24665 WP_160059458.1 5587173..5588873(+) (pilB) [Pseudomonas sp. R84]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAATTGGTGCAGGCCGAACTGCTCACGGAAAAAGGCGCCCAGCAAGC
CTGGCAACAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTGAAAAGCTCACAGGTCG
CCGAGATCGCTTCCGAGCACTTCGGCATGGCGTTCCTCGATCTCAACTGTCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTACGCCAGCATCACGCCCTGCCTTTGTGGCGACGCGGCAACAAGCTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGTACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGATGCCATCGAGAAGTTCTTCGACACCCACACCAGTGGCCTGGAAGACATGGCCGATGTCGATCTCGATGGC
CTCGATGTCGAATCAATCGACGACAGCAAACAGGAGGCCATCGGTGGCCTCGATGCCGACGATGCGCCGGTGGTGCGTTT
CGTTCACAAGATGCTGCTAGACGCGATCAAGAGCGGCTCATCCGACTTGCACTTCGAACCTTACGAGAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAGCCACCGGTTCAACTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTCCGCGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CGCAGATCGGCATCGACGCCCTTGGCTATGAACCAGAGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGGCCCACCGGATCGGGTAAAACCGTGTCGCTGTACACCGGCCTGAATATCCTCAACACCGTCGA
CATCAACATCTCCACCGCTGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAAG
GACTCGACTTTGCTCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCATACCAACAGCGCCGCGGA
AACTCTGACGCGCCTGCATAACATGGGCATTCCCGGTTTCAACATCGCCACGTCGGTCAGTCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAGAAGCCCATCGAGATCCCACGCGAAACTTTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACATTCAAA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2KXK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Acinetobacter baylyi ADP1

58.437

99.47

0.581
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.735

100

0.498
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilF Thermus thermophilus HB27

37.097

98.587

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362

Multiple sequence alignment