Detailed information NTG0215646

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: CV702_RS10185
• Genome accession: NZ_CP024954
• Coordinates: 2121376..2122452 (+)
• Length: 358 a.a.
• NCBI ID: WP_010906242.1
• Uniprot ID: Q9CE70
• Organism: Lactococcus lactis subsp. lactis strain F44
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2116376..2127452

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CV702_RS10160 (CV702_10170)	pgsA	2116484..2117056 (-)	573	WP_017865076.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
CV702_RS10165 (CV702_10175)	-	2117097..2117993 (-)	897	WP_017865077.1	helix-turn-helix domain-containing protein	-
CV702_RS10170 (CV702_10180)	yfmH	2118257..2119540 (-)	1284	WP_017865078.1	EF-P 5-aminopentanol modification-associated protein YfmH	-
CV702_RS10175 (CV702_10185)	yfmF	2119530..2120816 (-)	1287	WP_017865079.1	EF-P 5-aminopentanol modification-associated protein YfmF	-
CV702_RS10180 (CV702_10190)	yaaA	2121014..2121376 (+)	363	WP_012898492.1	S4 domain-containing protein YaaA	-
CV702_RS10185 (CV702_10195)	recF	2121376..2122452 (+)	1077	WP_010906242.1	DNA replication/repair protein RecF	Machinery gene
CV702_RS10190 (CV702_10200)	-	2122539..2122907 (-)	369	WP_017865080.1	carboxymuconolactone decarboxylase family protein	-
CV702_RS10195 (CV702_10205)	-	2122985..2123362 (+)	378	WP_017865081.1	MerR family transcriptional regulator	-
CV702_RS10200 (CV702_10210)	-	2123375..2124322 (-)	948	WP_026138958.1	alpha/beta hydrolase	-
CV702_RS10205 (CV702_10215)	galE	2124445..2125425 (-)	981	WP_017865083.1	UDP-glucose 4-epimerase GalE	-

Sequence

Protein

• Length: 358 a.a.
• Molecular weight: 41356.23 Da
• Isoelectric Point: 6.8652

>NTDB_id=215646 CV702_RS10185 WP_010906242.1 2121376..2122452(+) (recF) [Lactococcus lactis subsp. lactis strain F44]
MKLKAIELKNFRNYEELKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELISWSQQEMKVSGVVEKAHAT
VPLEVQLSPKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRKFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDKDKIDKNFLSVLDGQLAEHGNKIMLERQNFIDKLEVHAKKIHEQLTHGKENLKIIYNQNVKTDFSKELLSRQD
HDIFRHQTSVGPHRDDLQFFINEINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVNAGTIEQEQ

Nucleotide

• Length: 1077 bp

>NTDB_id=215646 CV702_RS10185 WP_010906242.1 2121376..2122452(+) (recF) [Lactococcus lactis subsp. lactis strain F44]
ATGAAACTCAAAGCAATTGAACTCAAAAATTTTCGTAATTATGAGGAATTAAAACTTGATTTTCATCCTAATCTCAATAT
TTTTTTAGGACAAAATGCTCAAGGGAAAACAAATATTCTTGAAGCGATTCATTTTTTAGCACTCACTCGTAGTCACAGAA
CAAGTCACGACAAAGAGCTCATTTCTTGGTCGCAACAAGAAATGAAGGTCTCAGGAGTCGTTGAAAAAGCTCATGCAACT
GTTCCACTTGAAGTTCAATTAAGTCCCAAAGGGAGAATTGCTAAAGCCAATCATCTCAAAGAAAATCGCTTAGCCGACTA
CATTGGTCAACTAAAAATCTTGATGTTTGCACCTGAAAATTTGGAATTGGTCAAAGGTTCTCCTGCTACCCGCAGAAAAT
TTATGGATATTGAACTGGGACAAATCCATGCGGTTTACCTTTATGATTCCATGCGATATAATCGTGCATTAAAAGAACGG
AATGCTTACTTAAAATTTGATAAAGACAAAATTGATAAAAATTTTTTAAGTGTTCTTGACGGACAATTAGCTGAACATGG
TAATAAAATCATGCTTGAGCGGCAAAATTTTATTGATAAACTTGAAGTTCATGCAAAAAAAATCCATGAGCAACTTACTC
ATGGTAAAGAAAATTTAAAAATCATTTATAATCAAAATGTGAAAACTGACTTTTCAAAAGAATTATTGAGCAGACAAGAC
CATGATATTTTTAGGCATCAAACATCGGTTGGCCCTCATCGTGACGATTTACAATTTTTTATCAATGAGATTAATGTTGC
TGATTTTGGTAGTCAAGGACAACAACGAACCGTTGCTCTCTCAATTAAATTAGCAGAAATTGATTTAATTTTTGAAGAGA
CTGGAGAGTATCCGATTTTACTTTTAGATGATGTCATGAGTGAATTAGACAATCATCGTCAACTTGATTTGATTGAAACA
AGTTTGGGTAAAACACAAACTTTTATAACTACAACTACTCTTGATCATCTTAAAAATTTACCTGAAAATCTGAGTATTTT
CCATGTTAATGCTGGAACCATTGAACAAGAGCAATAA

Domains

Predicted by InterProScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (3-353)

3D structure

• AlphaFold DB: Q9CE70

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Streptococcus pneumoniae R6	55	100	0.553
recF	Bacillus subtilis subsp. subtilis str. 168	45.977	97.207	0.447