Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO536_RS26415 Genome accession   NZ_CP019171
Coordinates   5823922..5825664 (+) Length   580 a.a.
NCBI ID   WP_012207286.1    Uniprot ID   -
Organism   MAG: Delftia acidovorans isolate ANG1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5818922..5830664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO536_RS26385 proB 5819440..5820588 (-) 1149 WP_012207281.1 glutamate 5-kinase -
  AO536_RS26390 cgtA 5820672..5821787 (-) 1116 WP_012207282.1 Obg family GTPase CgtA -
  AO536_RS26395 rpmA 5821937..5822194 (-) 258 WP_012207283.1 50S ribosomal protein L27 -
  AO536_RS26400 rplU 5822210..5822521 (-) 312 WP_012207284.1 50S ribosomal protein L21 -
  AO536_RS26405 - 5822752..5823681 (+) 930 WP_034396917.1 polyprenyl synthetase family protein -
  AO536_RS26415 pilB 5823922..5825664 (+) 1743 WP_012207286.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO536_RS26420 pilC 5825703..5826920 (+) 1218 WP_012207287.1 type II secretion system F family protein Machinery gene
  AO536_RS26425 pilD 5826920..5827804 (+) 885 WP_012207288.1 A24 family peptidase Machinery gene
  AO536_RS26430 coaE 5827819..5828463 (+) 645 WP_012207289.1 dephospho-CoA kinase -
  AO536_RS26435 zapD 5828553..5829308 (+) 756 WP_012207290.1 cell division protein ZapD -
  AO536_RS26440 - 5829326..5829562 (+) 237 WP_085930583.1 DNA gyrase inhibitor YacG -
  AO536_RS26445 - 5829573..5830091 (-) 519 WP_012207292.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62695.82 Da        Isoelectric Point: 5.3458

>NTDB_id=213629 AO536_RS26415 WP_012207286.1 5823922..5825664(+) (pilB) [MAG: Delftia acidovorans isolate ANG1]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AINAARLPHELLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASEA
MESYSGGGDFEFGDLKPDEVAEEKDNTAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLIEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLEAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=213629 AO536_RS26415 WP_012207286.1 5823922..5825664(+) (pilB) [MAG: Delftia acidovorans isolate ANG1]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGCATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTATCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCGGGCATCCTCTCAGCCCTGGAAGTGGCCGAGACGGTCTCGACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCATCAACGCCGCGCGCCTGCCGCATGAGTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCGTTGAGCAA
GCGCGGCAACCGCATCACGGTGGCCACAGCCGACCCGACACAACAGGAAGCTGCGGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAAGCCGACAAGCTGGAGCGCCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAGCC
ATGGAGAGCTACAGCGGGGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAGAAAGACAACAC
GGCGGCAGCGGACGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCCTACGAACATCAATATCGCGTACGCTTCCGCATTGACGGCGAGTTGCGCGAAATCGCATCA
CCGCCCATTGCCATAAAGGAGAAGTTGGCCTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAAAAACGTGTTCC
TCAAGATGGCCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGATTTTCGCGTGAGCACCCTGCCCACGCTCTTTG
GCGAGAAGATCGTGATCCGTATCCTTGACCCAAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCCGAGGAA
AAGGCACGTCTGATCGAAGCCATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACGGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCAACGGCAGAAGACCCTTCGGAAATCAACC
TGCCCGGCGTCAATCAGGTCAATGTGAACGAAAAGGCGGGCCTTACCTTTGCGACGGCGCTCAAGGCCTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGAGAAATCCGTGACCTAGAAACCGCCGACATCTCGATCAAGGCCGCACAGACCGGCCA
CTTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCATCGAGCGTGATCTTGATCACCGCCCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCGCTGCTGGAGGCGGGCTATGACGAGGCGGACATCGATGGCAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAAGCATGCAACAACGGCTACAAGGGACGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGCGCACTGGAGATCGCGGCACAGGCCAAGCGTGAAGGCGTACGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.536

98.103

0.545
  pilB Acinetobacter baumannii D1279779

56.028

97.241

0.545
  pilF Neisseria gonorrhoeae MS11

52.297

97.586

0.51
  pilB Legionella pneumophila strain ERS1305867

50.615

98.103

0.497
  pilB Vibrio cholerae strain A1552

46.461

95

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

45.554

95

0.433
  pilB Vibrio campbellii strain DS40M4

43.717

97.414

0.426
  pilF Thermus thermophilus HB27

38.204

97.931

0.374

Multiple sequence alignment