Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVL33_RS15780 Genome accession   NZ_CP019041
Coordinates   3278457..3280181 (+) Length   574 a.a.
NCBI ID   WP_075696849.1    Uniprot ID   A0AAW5RB14
Organism   Acinetobacter junii strain 65     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3273457..3285181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVL33_RS15745 (BVL33_15745) nusA 3274309..3275793 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  BVL33_RS15750 (BVL33_15750) rimP 3275831..3276355 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  BVL33_RS15770 (BVL33_15770) secG 3276978..3277307 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  BVL33_RS15775 (BVL33_15775) tpiA 3277320..3278111 (-) 792 WP_004958617.1 triose-phosphate isomerase -
  BVL33_RS15780 (BVL33_15780) pilB 3278457..3280181 (+) 1725 WP_075696849.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVL33_RS15785 (BVL33_15785) pilC 3280206..3281432 (+) 1227 WP_075696850.1 type II secretion system F family protein Machinery gene
  BVL33_RS15790 (BVL33_15790) pilD 3281432..3282292 (+) 861 WP_005403573.1 prepilin peptidase Machinery gene
  BVL33_RS15795 (BVL33_15795) coaE 3282294..3282893 (+) 600 WP_004958609.1 dephospho-CoA kinase -
  BVL33_RS15800 (BVL33_15800) - 3282890..3283801 (-) 912 WP_004958607.1 DMT family transporter -
  BVL33_RS15805 (BVL33_15805) rlmB 3283832..3284581 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BVL33_RS15810 (BVL33_15810) - 3284771..3285097 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63626.76 Da        Isoelectric Point: 5.1320

>NTDB_id=212825 BVL33_RS15780 WP_075696849.1 3278457..3280181(+) (pilB) [Acinetobacter junii strain 65]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSSCKQPIDIPKQSLL
EMGFTEQDLTTPGFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQ
GITSLQEVNRVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=212825 BVL33_RS15780 WP_075696849.1 3278457..3280181(+) (pilB) [Acinetobacter junii strain 65]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCTCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACGGGTTTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTAGAAACAGCTGAAATTGCTATCAAAGCAGCCCAAACAGGTCACATGGTGATGTCGACTTTGCA
CACCAATAGCGCTCCAGAAACACTCACACGTTTACGTAATATGGGAGTTCAGTCTTTCAATATTGCGACTTCGGTTAATC
TAGTAATTGCTCAACGTTTAGCTCGCCGCCTATGTTCTTCATGCAAACAACCTATCGATATTCCTAAACAAAGCCTTCTA
GAAATGGGTTTCACCGAACAAGACTTAACCACCCCAGGTTTCCAAATATTTCAACCTGTAGGCTGTGATGCTTGTCGTGA
AGGATATAAAGGACGTGTCGGTATTTATGAAGTGATGAAAGTGACACCAGAGATTTCCAAGATTATTATGGAAGATGGGA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCGATAATTTACGTCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.903

100

0.812
  pilB Acinetobacter baylyi ADP1

76.348

100

0.765
  pilB Legionella pneumophila strain ERS1305867

52.788

93.728

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.731

97.038

0.483
  pilF Neisseria gonorrhoeae MS11

50.274

95.296

0.479
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

50.87

90.07

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.253

100

0.368

Multiple sequence alignment