Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVG12_RS25155 Genome accession   NZ_CP019038
Coordinates   5160704..5162437 (-) Length   577 a.a.
NCBI ID   WP_075794772.1    Uniprot ID   -
Organism   Massilia putida strain 6NM-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5155704..5167437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG12_RS25145 - 5157588..5159441 (+) 1854 WP_169926838.1 beta-galactosidase -
  BVG12_RS25150 pilC 5159455..5160693 (-) 1239 WP_075794771.1 type II secretion system F family protein Machinery gene
  BVG12_RS25155 pilB 5160704..5162437 (-) 1734 WP_075794772.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVG12_RS25160 - 5162684..5163991 (-) 1308 WP_075794773.1 HlyC/CorC family transporter -
  BVG12_RS25165 - 5164078..5164893 (-) 816 WP_075794774.1 slipin family protein -
  BVG12_RS25170 - 5164898..5165263 (-) 366 WP_075794775.1 hypothetical protein -
  BVG12_RS35695 - 5165437..5167008 (-) 1572 WP_075794776.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 61857.37 Da        Isoelectric Point: 5.0936

>NTDB_id=212714 BVG12_RS25155 WP_075794772.1 5160704..5162437(-) (pilB) [Massilia putida strain 6NM-7]
MAAVLPNTNPGTALPGLARALVQAGRLTMPQADSLQKKASADKAGFIDILLGAGAVDARSLAVFCAETFGYPLLDLATLS
PDALPVNAIDPKLMQAQRVVALARRGNKMSVALSDPTNTQALDQIKFQSESTVEPVIVAHDALLALLANISKSAEQDLSE
LVGDEGDIEFAEEEPQAGAAEAAAAAAEVEDAPIVRFLNKVLMDAINLGASDIHFEPYEKQYRIRLRVDGVLRDHLAPPL
SIRDKLVSRIKVLAKLDISEKRVPQDGRMRLVLSPTKTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDSLGYDPDQKAL
LIDAITRPYGMVLVTGPTGSGKTVSLYSCLNLLNKPGINISTAEDPAEINLPGVNQVNVNEKAGLTFPVALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPLDIGRD
TLKAAGFREDDLDSDWKPYGPVGCERCLGSGYKGRVGIYQIMPITPAIEALILAHGNAMEIAAQAEAEGVKSLRRSGLMK
VKQGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=212714 BVG12_RS25155 WP_075794772.1 5160704..5162437(-) (pilB) [Massilia putida strain 6NM-7]
ATGGCAGCAGTCCTCCCCAACACCAACCCTGGCACTGCATTGCCCGGGCTGGCGCGTGCCCTCGTGCAGGCGGGACGCTT
GACCATGCCGCAGGCGGATTCGCTGCAAAAGAAGGCGAGTGCCGATAAAGCCGGTTTCATCGACATCTTGCTCGGCGCCG
GCGCCGTCGATGCGCGCTCCCTGGCTGTCTTCTGCGCCGAGACCTTCGGCTACCCGCTGCTCGACCTCGCCACCCTCAGC
CCGGACGCGCTGCCCGTCAACGCCATCGACCCCAAGCTGATGCAGGCGCAGCGCGTCGTCGCGCTGGCCCGGCGCGGCAA
CAAGATGTCGGTGGCGCTGTCCGACCCGACGAATACCCAGGCACTGGACCAGATCAAGTTCCAGAGCGAATCGACGGTCG
AACCGGTCATCGTCGCCCACGACGCCCTGCTTGCCCTGCTGGCGAACATTTCCAAGAGTGCCGAGCAGGACTTGAGCGAA
CTCGTCGGCGACGAAGGCGACATCGAATTCGCCGAGGAAGAACCGCAGGCGGGGGCGGCGGAAGCGGCCGCGGCCGCGGC
GGAAGTCGAGGATGCGCCGATCGTGCGCTTCCTGAACAAGGTCCTGATGGATGCCATCAACCTGGGCGCGTCGGACATCC
ACTTCGAACCGTACGAAAAACAGTACCGCATCCGCCTGCGCGTGGACGGCGTGCTGCGCGACCACCTGGCCCCGCCGCTG
TCGATCCGCGACAAGCTCGTCTCGCGCATCAAGGTGCTGGCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCAGGACGG
CCGCATGCGCCTCGTGCTGTCGCCCACGAAGACCATCGATTTCCGTGTGAGCACCTTGCCGACCCTGTTCGGCGAAAAGA
CGGTCATGCGTATCCTCGACGCGACGCAAGCCCAGATGGGCATCGACTCGCTCGGCTACGACCCGGACCAGAAAGCCCTC
CTCATCGATGCCATCACGCGCCCGTACGGCATGGTGCTCGTGACGGGTCCGACGGGCTCCGGTAAAACCGTGTCGCTGTA
CAGTTGCCTGAATTTGCTGAACAAGCCCGGGATCAATATCTCGACGGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCG
TGAACCAGGTCAACGTCAACGAAAAAGCGGGCCTCACGTTCCCCGTCGCGCTGAAAGCCTTTCTGCGCCAGGACCCGGAC
ATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCTGACATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGTT
TTCCACGCTGCACACGAACGACGCGCCGTCGACCCTGACGCGCCTGATGAATATGGGCGTGGCCCCGTTCAACATCGCCT
CGTCCGTCATCCTGATCACCGCCCAGCGCCTCGCGCGCCGCCTGTGCACGTGCAAGCAGCCGCTCGACATCGGGCGCGAC
ACGTTGAAGGCGGCCGGTTTCCGCGAAGACGACCTGGACAGCGACTGGAAGCCTTACGGCCCCGTCGGCTGCGAACGCTG
CCTCGGCTCCGGCTACAAGGGCCGCGTGGGCATCTACCAGATCATGCCGATCACGCCCGCGATCGAGGCCCTGATCCTGG
CGCACGGCAACGCAATGGAAATCGCCGCCCAGGCCGAGGCCGAGGGCGTGAAGTCGCTGCGCCGGTCCGGCCTCATGAAA
GTGAAGCAAGGCCTGACGAGCCTCGAGGAAGTGCTCGGCTGCACGAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.819

99.827

0.537
  pilB Acinetobacter baylyi ADP1

53.901

97.747

0.527
  pilF Neisseria gonorrhoeae MS11

53.451

97.92

0.523
  pilB Legionella pneumophila strain ERS1305867

49.733

97.227

0.484
  pilB Vibrio cholerae strain A1552

44.93

99.133

0.445
  pilB Vibrio campbellii strain DS40M4

44.561

98.787

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

44.938

97.574

0.438
  pilF Thermus thermophilus HB27

39.243

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.68

97.4

0.386

Multiple sequence alignment