Detailed information NTG0211748

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BVD86_RS10570
• Genome accession: NZ_CP018909
• Coordinates: 2196967..2198085 (+)
• Length: 372 a.a.
• NCBI ID: WP_060465360.1
• Uniprot ID: A0A2I6LWN2
• Organism: Acinetobacter pittii strain XJ88
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2191967..2203085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVD86_RS10560 (BVD86_10610)	yggS	2195091..2195783 (-)	693	WP_101663976.1	YggS family pyridoxal phosphate-dependent enzyme	-
BVD86_RS10565 (BVD86_10615)	pilT	2195910..2196947 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
BVD86_RS10570 (BVD86_10620)	pilU	2196967..2198085 (+)	1119	WP_060465360.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BVD86_RS10575 (BVD86_10625)	fur	2198155..2198592 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
BVD86_RS10580 (BVD86_10630)	omlA	2198704..2199102 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
BVD86_RS10585 (BVD86_10635)	-	2199167..2199481 (-)	315	WP_101663977.1	RnfH family protein	-
BVD86_RS10590 (BVD86_10640)	-	2199481..2200548 (-)	1068	WP_101663978.1	hypothetical protein	-
BVD86_RS10600 (BVD86_10650)	-	2200702..2201157 (-)	456	WP_002121698.1	bacteriohemerythrin	-
BVD86_RS10605 (BVD86_10655)	-	2201409..2202257 (-)	849	WP_057062207.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=211748 BVD86_RS10570 WP_060465360.1 2196967..2198085(+) (pilU) [Acinetobacter pittii strain XJ88]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=211748 BVD86_RS10570 WP_060465360.1 2196967..2198085(+) (pilU) [Acinetobacter pittii strain XJ88]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACGGGACACTTGGTCTTAGCCACAATGCATGCAAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTATAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A2I6LWN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363