Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BLE401_RS10130 Genome accession   NZ_CP018889
Coordinates   2426201..2427925 (+) Length   574 a.a.
NCBI ID   WP_062152443.1    Uniprot ID   -
Organism   Beggiatoa leptomitoformis strain D-401     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2421201..2432925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLE401_RS10110 (BLE401_14245) - 2421629..2422327 (-) 699 WP_066246125.1 glycine zipper domain-containing protein -
  BLE401_RS10115 (BLE401_14240) - 2422659..2423579 (+) 921 WP_062152439.1 AEC family transporter -
  BLE401_RS10120 (BLE401_14235) trpE 2423784..2425268 (+) 1485 WP_062152441.1 anthranilate synthase component I -
  BLE401_RS10125 (BLE401_14230) - 2425288..2425872 (+) 585 WP_201800074.1 anthranilate synthase component II -
  BLE401_RS10130 (BLE401_14225) pilB 2426201..2427925 (+) 1725 WP_062152443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BLE401_RS10135 (BLE401_14220) pilC 2427932..2429194 (+) 1263 WP_062152445.1 type II secretion system F family protein Machinery gene
  BLE401_RS10140 (BLE401_14215) pilD 2429200..2430099 (+) 900 WP_062152447.1 prepilin peptidase Machinery gene
  BLE401_RS10145 (BLE401_14210) coaE 2430096..2430716 (+) 621 WP_062152449.1 dephospho-CoA kinase -
  BLE401_RS10150 (BLE401_14205) zapD 2430745..2431524 (+) 780 WP_062152451.1 cell division protein ZapD -
  BLE401_RS10155 (BLE401_18635) - 2431637..2432455 (+) 819 WP_062152453.1 response regulator -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63632.63 Da        Isoelectric Point: 5.5480

>NTDB_id=211331 BLE401_RS10130 WP_062152443.1 2426201..2427925(+) (pilB) [Beggiatoa leptomitoformis strain D-401]
MSVTKSKVILTGLARKLVSENLLTEAVAEQAFEEAIKTRSSFVAYLVEKKLLGSKEVAHAASEELGVPLVDIENIDLNPD
VIKLIDEKLLKKHRALPLFKRGNRLFVAMSDPTNFPALDEIKFQTKMNTEAILVEEDKILKLMEKVAELFDTGMKLDGEL
GDLDLADEDAENGVKEEEKSDSEDAPIVKFVNKMLLDAIKLGASDLHFEPYEKFYRVRFRVDGMLRSVSNPPVGLNRKLA
ARIKVMSRLDVSERRVPQDGRIKLKISASKSMDFRVSTCPTLFGEKIVMRILDSSAAKLNIDQLGYEPEQKEQYLQALSN
PYGMVLVTGPTGSGKTVSLYTGVNILNKEDVNISTAEDPVEINLPGVNQVQVDERTGMSFAKALKAFLRQDPDIILVGEI
RDLETGGIAIKAASTGHMVMSTLHTNDAPQTLTRMIDMGIAPFAIATSVNLVMAQRLCRRLCSCKVEVDIPKKALLEEGF
KEQEVNDPNFKVYGPRPDGCEKCTNSGYKGRVGIYQVMPISDAMRRMIMEGKNAVDLADQAQKEGIPDLRQSGLKKVKDG
LTSLEEINRVVLKE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=211331 BLE401_RS10130 WP_062152443.1 2426201..2427925(+) (pilB) [Beggiatoa leptomitoformis strain D-401]
ATGTCCGTCACCAAATCTAAAGTCATTCTCACTGGATTAGCGCGTAAGTTGGTTAGCGAAAACCTCCTTACAGAAGCTGT
AGCAGAACAGGCTTTTGAAGAAGCCATTAAAACACGTAGCTCATTCGTTGCGTATCTTGTTGAAAAAAAACTACTCGGCA
GCAAAGAAGTTGCACATGCCGCTTCTGAAGAGCTAGGCGTTCCCTTAGTTGATATTGAAAATATTGATTTAAATCCTGAT
GTTATTAAGCTGATTGATGAAAAGTTACTCAAAAAACATCGTGCATTACCCTTGTTTAAACGGGGTAATCGTCTGTTTGT
TGCGATGTCTGATCCTACGAATTTTCCTGCGTTAGATGAGATTAAATTCCAAACCAAGATGAATACAGAAGCTATCTTGG
TAGAAGAAGATAAGATTTTAAAACTCATGGAAAAAGTCGCCGAGCTTTTTGATACGGGCATGAAGCTAGATGGCGAGTTA
GGTGATTTAGACTTAGCTGATGAAGATGCCGAAAATGGTGTAAAAGAAGAGGAAAAAAGCGATTCAGAAGACGCGCCTAT
TGTTAAATTCGTTAATAAAATGTTACTTGACGCAATCAAATTAGGGGCTTCTGACTTACATTTTGAACCCTACGAAAAAT
TCTATCGTGTCCGTTTTCGAGTTGATGGTATGCTTCGCTCGGTTTCTAATCCCCCTGTTGGGCTAAACCGTAAACTGGCT
GCACGTATTAAGGTTATGTCCCGTCTTGACGTATCTGAACGACGTGTTCCACAAGATGGACGTATCAAACTCAAAATTTC
AGCCAGTAAATCTATGGATTTTCGTGTCAGCACTTGCCCTACGCTATTTGGCGAAAAAATCGTTATGCGTATTTTGGACT
CCTCAGCGGCAAAGTTAAATATTGACCAACTAGGCTATGAACCTGAACAAAAAGAACAATACCTTCAAGCCTTATCAAAC
CCTTATGGCATGGTGCTGGTTACAGGACCAACAGGAAGCGGTAAAACCGTATCCTTATATACAGGGGTGAATATTCTTAA
TAAAGAAGATGTAAATATCTCCACCGCAGAAGACCCTGTGGAAATTAACTTACCCGGTGTGAATCAAGTTCAAGTGGATG
AGCGCACGGGTATGAGCTTCGCGAAAGCACTAAAAGCCTTTTTACGACAAGACCCTGATATTATTTTGGTAGGAGAAATT
CGGGATTTAGAAACGGGTGGGATTGCGATTAAAGCCGCATCCACAGGACACATGGTTATGTCCACTTTACATACCAATGA
TGCCCCCCAAACGCTAACCCGTATGATTGACATGGGAATTGCACCATTCGCAATTGCTACCTCAGTCAACCTAGTGATGG
CGCAACGACTCTGCCGCCGTCTATGTTCGTGTAAAGTTGAAGTAGATATTCCTAAAAAAGCACTGTTAGAAGAAGGATTT
AAAGAACAAGAAGTCAATGATCCAAACTTCAAAGTTTATGGTCCGCGTCCTGATGGTTGTGAAAAATGTACCAACTCTGG
CTATAAAGGGCGGGTCGGGATTTATCAAGTAATGCCTATTTCAGATGCAATGCGCCGTATGATTATGGAAGGTAAAAATG
CGGTGGATTTAGCAGACCAAGCGCAAAAAGAGGGCATCCCTGACTTGCGTCAATCTGGTTTAAAAAAGGTAAAAGACGGA
TTGACTTCCCTAGAAGAAATCAATCGCGTAGTTCTTAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.07

99.652

0.549
  pilB Acinetobacter baylyi ADP1

54.545

99.652

0.544
  pilB Legionella pneumophila strain ERS1305867

50.432

100

0.509
  pilF Neisseria gonorrhoeae MS11

49.821

97.561

0.486
  pilB Vibrio cholerae strain A1552

46.39

96.516

0.448
  pilB Vibrio campbellii strain DS40M4

43.958

99.477

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

44.346

98.606

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.571

91.463

0.371
  pilF Thermus thermophilus HB27

39.623

92.334

0.366

Multiple sequence alignment