Detailed information NTG0211333

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BLE401_RS10140
• Genome accession: NZ_CP018889
• Coordinates: 2429200..2430099 (+)
• Length: 299 a.a.
• NCBI ID: WP_062152447.1
• Uniprot ID: A0A2N9YH48
• Organism: Beggiatoa leptomitoformis strain D-401
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2424200..2435099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BLE401_RS10125 (BLE401_14230)	-	2425288..2425872 (+)	585	WP_201800074.1	anthranilate synthase component II	-
BLE401_RS10130 (BLE401_14225)	pilB	2426201..2427925 (+)	1725	WP_062152443.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BLE401_RS10135 (BLE401_14220)	pilC	2427932..2429194 (+)	1263	WP_062152445.1	type II secretion system F family protein	Machinery gene
BLE401_RS10140 (BLE401_14215)	pilD	2429200..2430099 (+)	900	WP_062152447.1	prepilin peptidase	Machinery gene
BLE401_RS10145 (BLE401_14210)	coaE	2430096..2430716 (+)	621	WP_062152449.1	dephospho-CoA kinase	-
BLE401_RS10150 (BLE401_14205)	zapD	2430745..2431524 (+)	780	WP_062152451.1	cell division protein ZapD	-
BLE401_RS10155 (BLE401_18635)	-	2431637..2432455 (+)	819	WP_062152453.1	response regulator	-
BLE401_RS10160 (BLE401_14195)	-	2432599..2432964 (+)	366	WP_002691300.1	response regulator	-
BLE401_RS10165 (BLE401_14190)	-	2433282..2434400 (+)	1119	WP_062152455.1	ABC transporter substrate-binding protein	-
BLE401_RS10170 (BLE401_14185)	-	2434414..2434938 (+)	525	WP_062152458.1	chemotaxis protein CheW	-

Sequence

Protein

• Length: 299 a.a.
• Molecular weight: 33063.51 Da
• Isoelectric Point: 6.2081

>NTDB_id=211333 BLE401_RS10140 WP_062152447.1 2429200..2430099(+) (pilD) [Beggiatoa leptomitoformis strain D-401]
METLAYIATNPLLAVFLIGILGLIVGSFLNVIIYRLPVMMERDWRKQCEEILQQPTSSVTEPVFNIAYPPSRCPHCGHQI
TALENIPILSYLWQRGQCTGCHASISSRYPIIEGVSALLAMITVWQAWSFLGFGLPLLGVVILTWALLALSMIDIDHQLL
PDDITLPFLWLGVLLNLLGFYPSVGLYNSIIGVMAGYMSLWSVYILFKLVTGKEGMGAGDFKLLAMLGAWMGWQALPVIV
ILSSLVGATFGILMIVVRGQDKSIPISFGPYLAMAGWIHLLWGDMLIHWYQTMMGVSIG

Nucleotide

• Length: 900 bp

>NTDB_id=211333 BLE401_RS10140 WP_062152447.1 2429200..2430099(+) (pilD) [Beggiatoa leptomitoformis strain D-401]
ATGGAAACATTGGCTTATATTGCAACAAATCCGCTTTTAGCTGTTTTCCTTATTGGAATATTAGGTTTAATTGTGGGAAG
CTTTTTAAATGTTATTATTTATCGCTTACCCGTTATGATGGAGCGAGATTGGCGCAAGCAGTGTGAGGAGATTCTACAGC
AACCAACATCTTCTGTTACAGAACCTGTTTTTAATATCGCTTATCCGCCCTCACGGTGTCCACATTGTGGACATCAAATT
ACTGCCTTAGAAAATATTCCTATTTTAAGTTATCTTTGGCAACGGGGACAGTGTACGGGCTGTCATGCGTCCATCTCTTC
GCGCTACCCGATTATTGAAGGGGTTAGTGCATTGCTTGCTATGATAACGGTTTGGCAAGCATGGTCGTTTCTTGGATTTG
GTTTACCTCTTTTGGGCGTTGTCATTCTCACATGGGCATTACTCGCCTTGAGCATGATAGATATAGACCATCAATTATTA
CCTGATGATATTACCCTGCCTTTTTTATGGTTAGGTGTTTTATTAAATCTATTAGGTTTTTATCCGTCCGTGGGACTGTA
TAACAGTATTATTGGTGTCATGGCGGGTTATATGAGTTTATGGTCGGTTTATATTCTCTTTAAATTAGTAACAGGCAAGG
AAGGGATGGGGGCTGGTGATTTTAAATTGCTTGCAATGCTTGGTGCATGGATGGGATGGCAAGCCCTACCTGTTATCGTC
ATATTATCTTCTCTAGTTGGTGCAACCTTTGGTATTTTAATGATTGTGGTACGTGGACAGGATAAAAGCATCCCCATTTC
CTTTGGACCCTATCTTGCGATGGCAGGATGGATTCACCTGTTATGGGGAGATATGCTGATTCATTGGTATCAAACAATGA
TGGGAGTAAGTATTGGTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (142-252)

3D structure

• AlphaFold DB: A0A2N9YH48

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.465	94.983	0.498
pilD	Vibrio campbellii strain DS40M4	47.945	97.659	0.468
pilD	Neisseria gonorrhoeae MS11	46.127	94.983	0.438
pilD	Acinetobacter baumannii D1279779	43.599	96.656	0.421
pilD	Acinetobacter nosocomialis M2	43.599	96.656	0.421