Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IMCC3135_RS28765 Genome accession   NZ_CP018632
Coordinates   6650606..6652303 (+) Length   565 a.a.
NCBI ID   WP_088920720.1    Uniprot ID   A0A2Z2NWR2
Organism   Granulosicoccus antarcticus IMCC3135     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6645606..6657303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMCC3135_RS28740 (IMCC3135_29080) - 6646348..6647262 (+) 915 WP_088920715.1 hypothetical protein -
  IMCC3135_RS28745 (IMCC3135_29085) - 6647259..6648020 (+) 762 WP_088920716.1 methyltransferase domain-containing protein -
  IMCC3135_RS28750 (IMCC3135_29090) - 6648005..6649192 (+) 1188 WP_088920717.1 glycosyltransferase -
  IMCC3135_RS35320 (IMCC3135_29095) - 6649465..6649881 (+) 417 WP_088920718.1 pilin -
  IMCC3135_RS35325 (IMCC3135_29100) - 6649958..6650380 (+) 423 WP_088920719.1 pilin -
  IMCC3135_RS28765 (IMCC3135_29105) pilB 6650606..6652303 (+) 1698 WP_088920720.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IMCC3135_RS28770 (IMCC3135_29110) pilC 6652307..6653524 (+) 1218 WP_088920721.1 type II secretion system F family protein Machinery gene
  IMCC3135_RS28775 (IMCC3135_29115) - 6653595..6654518 (+) 924 WP_088920722.1 prepilin peptidase -
  IMCC3135_RS28780 (IMCC3135_29120) coaE 6654565..6655173 (+) 609 WP_157736339.1 dephospho-CoA kinase -
  IMCC3135_RS28785 (IMCC3135_29125) mutT 6655166..6655567 (-) 402 WP_088920724.1 8-oxo-dGTP diphosphatase MutT -
  IMCC3135_RS28790 (IMCC3135_29130) argJ 6655571..6656803 (-) 1233 WP_088922137.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61606.57 Da        Isoelectric Point: 4.8582

>NTDB_id=209191 IMCC3135_RS28765 WP_088920720.1 6650606..6652303(+) (pilB) [Granulosicoccus antarcticus IMCC3135]
MTTSSTLNGIARQLVNQGLVNEETAIKAIEHCKKSKTAFLSSLVLNDSVNSKDIAHYVASDFGLPLFDLATLAPESIPKD
LIKEELINSQNAVPIAQRGSRLFIAISDPTNNEPLEKYKFSSGLSVEAVVVDETALAELRSRVLDQSSDLDEGLDESFDL
EVDNGDMGDLDADDSGIDETPVVRFVNKVLLDAIKKGASDIHIEPYEKIFRIRFRVDGVLEEVVHPPLAMAGRLTARIKV
MSRMDISERRVPQDGRIKLKISKTKAIDFRVNTCPTLFGEKTVLRILDPSSAQLGIDALGYEPEQKKLYMDALANPYGMI
LVTGPTGSGKTVSLYTGLNILNEPGTNISTAEDPAEINLAGINQVNVNVKAGLTFAEALKSFLRQDPDVIMVGEIRDLET
ASIAVKAAQTGHLVLSTLHTNDAPQTLSRLINMGVPPFNIATAVSLIIAQRLARRLCEHCKVPDTDLPRELLLQEGFKEE
TVDELNIFKAVGCKKCNNGYKGRVGIYQVMKISEEISKIIMAGGNAIDIASQAKAEEIPDLRESALKKVAQGVMSLEEAN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=209191 IMCC3135_RS28765 WP_088920720.1 6650606..6652303(+) (pilB) [Granulosicoccus antarcticus IMCC3135]
ATGACAACCAGCAGTACGTTGAACGGCATTGCCAGGCAACTCGTTAATCAGGGACTCGTTAACGAAGAAACTGCCATCAA
GGCAATTGAGCACTGCAAGAAATCCAAAACGGCATTTCTAAGCAGCCTGGTGTTGAACGACTCGGTCAATTCGAAGGACA
TAGCCCACTATGTGGCCTCGGATTTTGGACTGCCGTTATTTGATTTGGCAACTCTGGCACCAGAGAGCATTCCCAAGGAC
TTGATCAAGGAAGAACTGATCAACTCACAGAATGCGGTGCCGATTGCGCAGCGAGGATCACGCCTCTTCATTGCCATCTC
AGACCCAACAAACAACGAGCCGCTGGAAAAATACAAGTTTTCATCCGGTTTGAGTGTTGAAGCGGTGGTGGTGGACGAAA
CTGCCCTTGCAGAGCTTCGATCACGTGTACTGGATCAAAGCTCGGATCTGGATGAAGGTCTGGACGAGAGTTTCGACCTG
GAAGTCGACAATGGTGACATGGGAGATCTGGATGCAGATGATTCCGGGATTGATGAGACACCGGTCGTACGCTTCGTCAA
CAAGGTGCTTCTGGATGCTATCAAGAAGGGCGCATCGGATATTCATATAGAGCCTTATGAGAAAATTTTTCGTATCCGCT
TTCGCGTCGATGGTGTGCTCGAAGAAGTCGTACACCCGCCATTGGCTATGGCAGGTCGTCTGACCGCACGCATCAAGGTC
ATGTCACGGATGGATATCTCCGAGCGGCGTGTGCCACAAGATGGTCGCATCAAGCTGAAGATCTCCAAGACCAAGGCGAT
CGATTTTCGAGTCAATACCTGCCCTACTCTGTTCGGCGAAAAAACGGTATTGCGTATTCTCGATCCTAGTAGTGCGCAGC
TAGGCATCGATGCACTGGGTTATGAGCCTGAACAGAAAAAGCTCTACATGGATGCGCTGGCCAATCCCTACGGCATGATT
CTGGTAACCGGTCCTACCGGTAGTGGTAAAACGGTCTCTTTGTATACCGGGCTGAATATTCTTAATGAACCTGGCACCAA
CATCTCCACCGCGGAGGATCCAGCGGAGATCAACCTTGCTGGTATCAACCAGGTCAATGTCAATGTCAAAGCAGGCCTGA
CTTTCGCAGAAGCTCTGAAGTCCTTCCTGCGACAGGATCCGGACGTCATCATGGTTGGTGAGATTCGTGATCTGGAGACA
GCAAGTATTGCGGTCAAAGCGGCGCAAACAGGTCATCTGGTCTTGTCCACACTACATACCAATGATGCGCCACAGACTCT
GTCTCGCCTCATTAACATGGGCGTGCCGCCCTTCAATATTGCAACTGCAGTATCACTGATCATCGCCCAGCGTCTGGCAC
GTCGATTATGTGAGCACTGCAAGGTGCCAGATACGGATCTTCCTCGTGAGCTGCTTCTACAAGAAGGTTTCAAGGAAGAG
ACGGTCGACGAACTCAATATATTCAAGGCCGTTGGCTGCAAAAAATGCAATAACGGCTACAAGGGTCGAGTCGGTATTTA
CCAGGTCATGAAGATCAGTGAAGAGATCAGCAAGATCATCATGGCAGGCGGTAATGCAATCGATATCGCCTCACAAGCCA
AGGCAGAAGAAATTCCCGACCTGCGTGAGTCAGCCCTGAAAAAGGTTGCTCAAGGCGTCATGAGTCTTGAAGAAGCCAAC
CGAGTGACAAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(14-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z2NWR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.923

100

0.582
  pilB Acinetobacter baumannii D1279779

56.766

100

0.572
  pilB Legionella pneumophila strain ERS1305867

51.943

100

0.52
  pilF Neisseria gonorrhoeae MS11

51.515

99.292

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.53

100

0.506
  pilB Vibrio cholerae strain A1552

52.5

92.035

0.483
  pilB Vibrio campbellii strain DS40M4

49.908

95.752

0.478
  pilF Thermus thermophilus HB27

37.746

98.938

0.373

Multiple sequence alignment