Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BI313_RS09620 Genome accession   NZ_CP018470
Coordinates   1910303..1912036 (-) Length   577 a.a.
NCBI ID   WP_039421899.1    Uniprot ID   A0AAJ0J1P3
Organism   Xanthomonas vesicatoria strain LM159     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1905303..1917036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI313_RS09605 (BI313_09530) sucC 1905335..1906504 (-) 1170 WP_005992833.1 ADP-forming succinate--CoA ligase subunit beta -
  BI313_RS09610 (BI313_09535) - 1906736..1908349 (+) 1614 WP_039421903.1 HAMP domain-containing sensor histidine kinase -
  BI313_RS09615 (BI313_09540) pilR 1908709..1910097 (+) 1389 WP_196769990.1 sigma-54 dependent transcriptional regulator Regulator
  BI313_RS09620 (BI313_09545) pilB 1910303..1912036 (-) 1734 WP_039421899.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI313_RS09625 (BI313_09550) - 1912176..1912775 (-) 600 WP_039427843.1 methionine biosynthesis protein MetW -
  BI313_RS09630 (BI313_09555) - 1912768..1913814 (-) 1047 WP_052318575.1 glycosyltransferase family 2 protein -
  BI313_RS09635 (BI313_09560) - 1913811..1914605 (-) 795 WP_039421896.1 glycosyltransferase family A protein -
  BI313_RS23970 - 1914610..1915251 (-) 642 WP_080764189.1 EamA family transporter -
  BI313_RS09645 (BI313_09570) - 1915335..1915751 (+) 417 WP_039421894.1 pilin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62826.97 Da        Isoelectric Point: 5.4486

>NTDB_id=208838 BI313_RS09620 WP_039421899.1 1910303..1912036(-) (pilB) [Xanthomonas vesicatoria strain LM159]
MNSVVTANLVGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANSVEFGMPLLDVSSFDPN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=208838 BI313_RS09620 WP_039421899.1 1910303..1912036(-) (pilB) [Xanthomonas vesicatoria strain LM159]
ATGAATAGCGTCGTTACCGCCAACTTGGTTGGCATCACGGGCATTGCCCGGCGGCTTGTGCAGGACGGTGCTCTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCTGCTACTGCCAAGGTCCCGTTGCCGCAATGGTTCTCAGAGAAGAAGCTGG
TGACCGCTTCGCAACTAGCAGCTGCCAACTCGGTGGAGTTCGGGATGCCGCTACTTGATGTGTCATCGTTCGACCCTAAT
CAGAATGCAATCAAGTTGGTTAGCGAAGAATTGCTGCAGAAGTTTCAAGTGCTGCCCCTGTTCAAGCGCGGTAATCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGAGCGCTGGACGACATCAAGTTCCATACCAACCTAGTGGTCGAGCCGA
TCCTGGTTGATGAGGACCAGATCCGGCGCACGTTGGAGCAGTGGCAGGCTAGCAATGCGGCGCTTGGCTCGTCGATGGGT
GATGACGATGATGAGATGGGGGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGGTTGCTGAAAAATGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCAGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAAAAGCGGGTACCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACGCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGATGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTTTTGGACGCGATCCACAAACCGTACGGCATGGTGTTGGTGACCGGGCCAACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGTTGGGCATCCTCAACGATGAGACGCGCAATATTTCAACCGCCGAGGATCCGGTCGAAATTCGGTTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACCTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCATACCAATGACGCCCCGCAGACCATTGCGCGTTTGATGAACATGGGCATCGCGCCGTACAACATCA
CTTCGTCGGTGACCCTGGTCATCGCGCAGCGTTTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCTTGCCG
GAGCATGCGCTGCTGGCAGAAGGGTTTACGCCAGAGCAAGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGCATTCGCGACTTGCGTCAGTCGGCATTGATGAAG
GCTGCGCACGGAGTGACCAGCCTGGCCGAGATCAATCGCGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.81

98.44

0.549
  pilB Acinetobacter baumannii D1279779

55.634

98.44

0.548
  pilB Legionella pneumophila strain ERS1305867

51.585

98.44

0.508
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio cholerae strain A1552

49.365

95.494

0.471
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.648

97.574

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

35.902

100

0.38

Multiple sequence alignment