Detailed information NTG0020774

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: L_RS11105
• Genome accession: NC_002662
• Coordinates: 2185661..2186107 (-)
• Length: 148 a.a.
• NCBI ID: WP_164752712.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis Il1403
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2180661..2191107

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L_RS11070 (L164222)	-	2180778..2181155 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
L_RS11075 (L164789)	-	2181360..2182226 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
L_RS11080 (L165709)	-	2182265..2183074 (-)	810	WP_010906310.1	metal ABC transporter permease	-
L_RS11085 (L166512)	-	2183067..2183804 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
L_RS11090 (L167426)	-	2183981..2184823 (-)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
L_RS11095 (L168265)	-	2184820..2185257 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
L_RS11100 (L168783)	comGG	2185338..2185622 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
L_RS11105 (L169106)	comGF	2185661..2186107 (-)	447	WP_164752712.1	competence type IV pilus minor pilin ComGF	Machinery gene
L_RS11110 (L169515)	comGE	2186070..2186366 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
L_RS11115 (L169783)	comGD	2186338..2186754 (-)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
L_RS11120 (L0315)	comGC	2186729..2187112 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
L_RS11125 (L0314)	comGB	2187126..2188199 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
L_RS11130 (L0313)	comGA	2188093..2189031 (-)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=20774 L_RS11105 WP_164752712.1 2185661..2186107(-) (comGF) [Lactococcus lactis subsp. lactis Il1403]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVALSQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=20774 L_RS11105 WP_164752712.1 2185661..2186107(-) (comGF) [Lactococcus lactis subsp. lactis Il1403]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGCTTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (46-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	74.82	93.919	0.703
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385