Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JN853_RS05515 Genome accession   NZ_CP018202
Coordinates   1086803..1088497 (-) Length   564 a.a.
NCBI ID   WP_003381201.1    Uniprot ID   A0A2V0QF19
Organism   Pseudomonas syringae pv. actinidiae ICMP 9853     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1081803..1093497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN853_RS34095 (JN853_05400) - 1083309..1083617 (+) 309 WP_025991960.1 hypothetical protein -
  JN853_RS05495 (JN853_05405) yacG 1083883..1084092 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  JN853_RS05500 (JN853_05410) coaE 1084089..1084712 (-) 624 WP_003381205.1 dephospho-CoA kinase -
  JN853_RS05505 (JN853_05415) pilD 1084709..1085581 (-) 873 WP_003381203.1 prepilin peptidase Machinery gene
  JN853_RS05510 (JN853_05420) pilC 1085583..1086800 (-) 1218 WP_003381202.1 type II secretion system F family protein Machinery gene
  JN853_RS05515 (JN853_05425) pilB 1086803..1088497 (-) 1695 WP_003381201.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JN853_RS05520 (JN853_05430) - 1088723..1089115 (+) 393 WP_003381200.1 pilin -
  JN853_RS05525 (JN853_05435) - 1089161..1090588 (+) 1428 WP_017699050.1 O-antigen ligase family protein -
  JN853_RS05530 (JN853_05440) - 1090701..1091987 (+) 1287 WP_017699051.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  JN853_RS05535 (JN853_05445) - 1092068..1092424 (-) 357 WP_003381197.1 winged helix-turn-helix transcriptional regulator -
  JN853_RS05540 (JN853_05450) - 1092581..1093186 (+) 606 WP_019717042.1 glutathione S-transferase N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62539.83 Da        Isoelectric Point: 6.2561

>NTDB_id=207205 JN853_RS05515 WP_003381201.1 1086803..1088497(-) (pilB) [Pseudomonas syringae pv. actinidiae ICMP 9853]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLAGLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLANQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=207205 JN853_RS05515 WP_003381201.1 1086803..1088497(-) (pilB) [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGTCTGATGCTGTCCTCACTGGTTTGGCCAAACAATTGGTCCTGGCCGAGTTACTTACTGAAACCGCTGCGCAGCAGGC
TTACCAACAGGCGCGCCGCGACAAGGTTTCACTGGTGAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTTG
CAGAAATGGCTTCGGACCAGTTTGGCGTGCCCTTCATGGATCTTGCCGGTCTGGACAAGGAAAGCCAGCCCAAGGGGCTG
GTCAGTGAAAAGCTGGTCCGTCAGCATCATGCGTTGCCACTTTGGCGGCGCGGTAACAAGCTGTTTATAGGCATATCGGA
CCCGACCAACCACCAGGCCGTCACAGACATACAGTTCAGCACCGGCCTGAATACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGCGGCCTTGGCGAGTTGGCAGACATCGACCTTGGCCTTGAT
ATCGAGCCTGCGAGTGACAAGGAAACGTCACTGGCAAATCAGAGTGATGCCGATGATGCCCCGGTCGTGCGCTTCGTCAA
CAAGATGCTGATGGACGCCATACGGCTCGGTTCGTCCGACCTGCACTTTGAACCCTACGAAAAGATCTTTCGCGTGCGCC
TGCGTACCGACGGCATTTTGCATGAAGTGGCCAGGCCACCGATCCATCTGGCCAACCGGATTGCTGCACGGCTTAAGGTA
ATGGCCAGTCTCGATATCTCTGAGCGGCGTAAACCGCAGGACGGCAGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCGAT
CGACTTTCGCATGAACACGCTGCCAACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCGACCAGCGCGCAGA
TGGGCATTGATGCACTGGGCTACGAACCGGAGCAAAAGGCGCTTTATATGGAGGCCTTGAAACAGCCCCAAGGCATGATT
CTTGTCACCGGCCCAACTGGGTCAGGCAAAACCGTTTCGCTATACACCGGTCTGAACATTCTCAACACTGTAGATATCAA
CATTTCCACAGCTGAAGACCCGGTAGAGATCAACCTGGAGGGTATCAATCAGGTCAATGTCAATCCGCGACAGGGCCTTG
ACTTCTCCCAGGCATTGCGTGCCTTTCTGCGTCAAGACCCCGACGTGATCATGGTCGGCGAAATTCGGGACCTGGAAACG
GCCGAAATTGCCATCAAAGCCTCGCAAACCGGTCACATGGTGTTATCGACCCTGCACACCAACAGCGCTGCAGAAACCCT
GACTCGCCTGCACCACATGGGCGTCGCAGCGTTCAACATCGCTACGGCAATCAACCTTATCATTGCCCAGCGGCTGGCAC
GCAAGCTATGCAGTCATTGCAAGAAAGAACTCGACATACCTCGTGAAACACTCATACGAGAGGGCTTCCCGGAAGAAAAG
ATAGGTACGTTCAAAATCTACGGCCCTGTTGGCTGTGAGCATTGCAATGGCGGTTACAGGGGCAGGGTCGGGATCTACGA
AGTAGTCAAGAAAACACCTGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGAA
AGGACGGCTTCAACGATTTGAGGACCTCCGGCCTGTCGAAGGCCATGCAAGGCATAACTAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V0QF19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

100

0.585
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.221

100

0.553
  pilB Vibrio cholerae strain A1552

50.624

99.468

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.181

98.227

0.493
  pilB Vibrio campbellii strain DS40M4

49.113

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.399

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369

Multiple sequence alignment