Detailed information NTG0207203

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JN853_RS05505
• Genome accession: NZ_CP018202
• Coordinates: 1084709..1085581 (-)
• Length: 290 a.a.
• NCBI ID: WP_003381203.1
• Uniprot ID: A0A2V0QGS2
• Organism: Pseudomonas syringae pv. actinidiae ICMP 9853
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1079709..1090581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JN853_RS05460 (JN853_05375)	-	1080384..1081196 (+)	813	WP_017682620.1	lysophospholipid acyltransferase family protein	-
JN853_RS05465 (JN853_05380)	-	1081171..1081431 (+)	261	WP_002551687.1	phosphopantetheine-binding protein	-
JN853_RS05470 (JN853_05385)	-	1081441..1081695 (+)	255	WP_017698950.1	acyl carrier protein	-
JN853_RS34095 (JN853_05400)	-	1083309..1083617 (+)	309	WP_025991960.1	hypothetical protein	-
JN853_RS05495 (JN853_05405)	yacG	1083883..1084092 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
JN853_RS05500 (JN853_05410)	coaE	1084089..1084712 (-)	624	WP_003381205.1	dephospho-CoA kinase	-
JN853_RS05505 (JN853_05415)	pilD	1084709..1085581 (-)	873	WP_003381203.1	prepilin peptidase	Machinery gene
JN853_RS05510 (JN853_05420)	pilC	1085583..1086800 (-)	1218	WP_003381202.1	type II secretion system F family protein	Machinery gene
JN853_RS05515 (JN853_05425)	pilB	1086803..1088497 (-)	1695	WP_003381201.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JN853_RS05520 (JN853_05430)	-	1088723..1089115 (+)	393	WP_003381200.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32020.33 Da
• Isoelectric Point: 8.3782

>NTDB_id=207203 JN853_RS05505 WP_003381203.1 1084709..1085581(-) (pilD) [Pseudomonas syringae pv. actinidiae ICMP 9853]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKAQSREMLGLPAVPDQPVFNLILPHSSCPHCAHKIR
PWENLPIISYLLLRGKCSHCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLMG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=207203 JN853_RS05505 WP_003381203.1 1084709..1085581(-) (pilD) [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGCCCCTCCTCGACCTCCTGGCAAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCCTGCTCGGCCTCATCGTCGGAAG
CTTCCTCAACGTAGTCGTTTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGCGCAATCACGGGAAATGCTCGGCC
TCCCTGCCGTACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCCAGTTGCCCGCACTGCGCGCACAAGATCCGC
CCATGGGAAAATCTGCCGATCATCAGTTACCTGTTGCTGCGCGGCAAATGCTCACACTGCAAAGCACCGATCAGCAAGCG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTATCGGCCTACGTCGCCTGGCACTTCGGCTTTGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGCTGGGGTTTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTGCCTGACTCTCTGGTA
CTTCCACTGCTGTGGCTGGGCCTGATCGTTAACGCCTTTGGGCTGTTCACCTCGCTGAATGATGCGTTGTGGGGCGCAGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGTTGTCGTCGCTGATGGGT
GCGGTGCTGGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A2V0QGS2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.417	97.586	0.531
pilD	Vibrio campbellii strain DS40M4	51.237	97.586	0.5
pilD	Acinetobacter baumannii D1279779	51.625	95.517	0.493
pilD	Acinetobacter nosocomialis M2	51.264	95.517	0.49
pilD	Neisseria gonorrhoeae MS11	52.239	92.414	0.483