Detailed information NTG0204413

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAMY_RS12650
• Genome accession: NZ_CP017953
• Coordinates: 2570717..2571331 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain Y14
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2565717..2576331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMY_RS12625 (BAMY_12355)	rpsT	2566017..2566283 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAMY_RS12630 (BAMY_12360)	holA	2566300..2567341 (-)	1042	Protein_2444	DNA polymerase III subunit delta	-
BAMY_RS20075	-	2567381..2567535 (-)	155	Protein_2445	hypothetical protein	-
BAMY_RS12635 (BAMY_12365)	-	2567576..2567710 (+)	135	WP_003152870.1	YqzM family protein	-
BAMY_RS12640 (BAMY_12370)	comEC	2567729..2570080 (-)	2352	WP_025852986.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAMY_RS12645 (BAMY_12375)	-	2570081..2570650 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAMY_RS12650 (BAMY_12380)	comEA	2570717..2571331 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAMY_RS12655 (BAMY_12385)	comER	2571390..2572211 (+)	822	WP_003152866.1	late competence protein ComER	-
BAMY_RS12660 (BAMY_12390)	-	2572280..2573017 (-)	738	WP_025852989.1	class I SAM-dependent DNA methyltransferase	-
BAMY_RS12665 (BAMY_12395)	rsfS	2573014..2573370 (-)	357	WP_003152864.1	ribosome silencing factor	-
BAMY_RS12670 (BAMY_12400)	yqeK	2573367..2573948 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAMY_RS12675 (BAMY_12405)	-	2573938..2574507 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
BAMY_RS12680 (BAMY_12410)	yhbY	2574518..2574808 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAMY_RS12685 (BAMY_12415)	aroE	2574802..2575644 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=204413 BAMY_RS12650 WP_003152867.1 2570717..2571331(-) (comEA) [Bacillus amyloliquefaciens strain Y14]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=204413 BAMY_RS12650 WP_003152867.1 2570717..2571331(-) (comEA) [Bacillus amyloliquefaciens strain Y14]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363