Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BKK81_RS17485 Genome accession   NZ_CP017751
Coordinates   3948323..3950059 (+) Length   578 a.a.
NCBI ID   WP_071015563.1    Uniprot ID   -
Organism   Cupriavidus sp. USMAHM13 isolate pure     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3943323..3955059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK81_RS17460 (BKK81_17460) obgE 3944500..3945600 (-) 1101 WP_071015558.1 GTPase ObgE -
  BKK81_RS17465 (BKK81_17465) rpmA 3945743..3946003 (-) 261 WP_071015560.1 50S ribosomal protein L27 -
  BKK81_RS17470 (BKK81_17470) rplU 3946064..3946375 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  BKK81_RS17475 (BKK81_17475) ispB 3947020..3947949 (+) 930 WP_071015561.1 octaprenyl diphosphate synthase -
  BKK81_RS17485 (BKK81_17485) pilF 3948323..3950059 (+) 1737 WP_071015563.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKK81_RS17490 (BKK81_17490) pilC 3950088..3951353 (+) 1266 WP_071015565.1 type II secretion system F family protein Machinery gene
  BKK81_RS17495 (BKK81_17495) pilD 3951357..3952250 (+) 894 WP_071015568.1 A24 family peptidase Machinery gene
  BKK81_RS17500 (BKK81_17500) coaE 3952305..3952937 (+) 633 WP_071015570.1 dephospho-CoA kinase -
  BKK81_RS17505 (BKK81_17505) zapD 3953145..3953903 (+) 759 WP_071016592.1 cell division protein ZapD -
  BKK81_RS17510 (BKK81_17510) - 3953988..3954179 (+) 192 WP_071015571.1 DNA gyrase inhibitor YacG -
  BKK81_RS17515 (BKK81_17515) - 3954220..3954648 (-) 429 WP_071015573.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63322.88 Da        Isoelectric Point: 7.8029

>NTDB_id=202525 BKK81_RS17485 WP_071015563.1 3948323..3950059(+) (pilF) [Cupriavidus sp. USMAHM13 isolate pure]
MTLGLALAQSRRIAPALLAQLEQAAREKKSQLIDEIVGSGTMSARDLATFAADKYQLPLLDLSQFNFATVPPALVGNREF
HAHRLLPLGRRDNRLVVATSDPSNQAGLDAIRQKLNLPVDAVVVEHDKLLKHVRSAGEESSTLRAAGPVAAEHKLIEYDP
VASAGAQRNKAPNDIDDAPVVRFLHKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLLEVARPPLDIRDKIATRIKVLS
RLDISEKRVPQDGRMKLLIALPKEKDKDGKDSKETVEKAIDFRVSTLPTLFGEKIVMRILDSSTDKLDIDRLGYEPAQKA
LLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNEKAGLTFAAALRSFLRQDP
DVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCNCKRPGALAR
DVLLDAGFQESELDGSWQPYHPAGCERCSGSGYKGRCGIYQVMPISEAMQQIILSHGTALQIAEQARRDGVLSLREAGLL
KVKQGVTSLEEVLATTNM

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=202525 BKK81_RS17485 WP_071015563.1 3948323..3950059(+) (pilF) [Cupriavidus sp. USMAHM13 isolate pure]
ATGACACTAGGTCTCGCTCTCGCGCAAAGCCGCCGCATCGCTCCGGCCCTTCTCGCCCAGCTCGAACAGGCCGCGCGGGA
AAAGAAGTCGCAACTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCGCGACCTCGCCACCTTCGCCGCCGACA
AGTACCAGCTCCCGCTGCTCGACCTCAGCCAGTTCAACTTCGCCACGGTGCCGCCGGCCCTGGTCGGCAACCGCGAGTTC
CACGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGACAACCGGCTGGTCGTGGCAACCTCCGATCCGTCCAACCAGGCCGG
CCTCGATGCCATCCGCCAGAAACTGAACCTGCCGGTCGACGCGGTCGTGGTCGAGCACGACAAGCTGCTGAAGCACGTGC
GCTCGGCCGGCGAGGAAAGCAGTACGCTGCGCGCCGCCGGCCCGGTGGCGGCCGAGCACAAGCTGATCGAGTACGACCCG
GTCGCCTCGGCGGGAGCCCAGCGCAACAAGGCGCCCAACGACATCGACGACGCACCGGTCGTGCGCTTCCTGCACAAGCT
GTTGACCGAGGCCTTCCACCGGGGTGCATCCGACCTGCACTTCGAGCCGTTCGAGACCTTCTACCGCGTGCGCTTCCGCG
TCGACGGCGTGCTGCTGGAGGTGGCCCGCCCCCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCG
CGCCTGGACATCTCCGAGAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCGAAGGAAAAGGACAA
GGACGGCAAGGACAGCAAGGAGACGGTCGAGAAGGCCATCGATTTCCGTGTGTCGACGCTGCCGACACTGTTCGGCGAGA
AGATCGTCATGCGGATCCTCGACTCCTCGACCGACAAGCTCGACATCGACCGGCTCGGCTACGAGCCCGCGCAGAAGGCG
CTGCTGCTCGAGACCATCAAGCGCCCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCAGCGGCAAGACGGTCTCGCT
CTATACCTTCCTCAACCTGCTCAACCAGGGCGACATCAATATCTCGACGGCCGAGGATCCCGCCGAAATCCAGCTGCCCG
GCATCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTGACCTTCGCCGCCGCGCTGCGCTCCTTCCTGCGCCAGGACCCG
GACGTCATCATGGTGGGCGAGATCCGCGACCTGGAGACCGCGGACATCGCCATCAAGGCCGCCCAGACCGGCCATCTGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGACCACGCTGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCG
CCTCCAGCGTGCTGCTGATCACCGCCCAGCGGCTGGCGCGCCGGCTCTGCAACTGCAAGCGGCCGGGCGCCCTGGCGCGC
GACGTGCTGCTTGACGCCGGCTTCCAAGAGAGCGAACTCGACGGCAGCTGGCAGCCTTACCACCCGGCCGGCTGCGAGCG
CTGCAGCGGCAGCGGCTACAAGGGGCGTTGCGGCATCTACCAGGTGATGCCCATCTCCGAGGCGATGCAGCAGATCATCC
TGTCTCACGGTACGGCGCTGCAGATCGCCGAGCAGGCGCGCCGCGACGGCGTGCTATCGTTGCGGGAAGCCGGCCTGCTG
AAGGTGAAGCAAGGCGTCACCTCGCTGGAAGAAGTGCTGGCGACGACCAATATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-136)

Type II/IV secretion system protein (T2SSE)

(186-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.853

100

0.516
  pilB Acinetobacter baumannii D1279779

50.685

100

0.512
  pilB Acinetobacter baylyi ADP1

51.434

96.54

0.497
  pilB Legionella pneumophila strain ERS1305867

49.464

96.886

0.479
  pilB Vibrio cholerae strain A1552

46.367

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

47.145

93.945

0.443
  pilB Vibrio campbellii strain DS40M4

45.173

94.983

0.429
  pilF Thermus thermophilus HB27

38.382

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.188

97.405

0.372

Multiple sequence alignment