Detailed information NTG0202527

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BKK81_RS17495
• Genome accession: NZ_CP017751
• Coordinates: 3951357..3952250 (+)
• Length: 297 a.a.
• NCBI ID: WP_071015568.1
• Uniprot ID: -
• Organism: Cupriavidus sp. USMAHM13 isolate pure
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3946357..3957250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKK81_RS17475 (BKK81_17475)	ispB	3947020..3947949 (+)	930	WP_071015561.1	octaprenyl diphosphate synthase	-
BKK81_RS17485 (BKK81_17485)	pilF	3948323..3950059 (+)	1737	WP_071015563.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BKK81_RS17490 (BKK81_17490)	pilC	3950088..3951353 (+)	1266	WP_071015565.1	type II secretion system F family protein	Machinery gene
BKK81_RS17495 (BKK81_17495)	pilD	3951357..3952250 (+)	894	WP_071015568.1	A24 family peptidase	Machinery gene
BKK81_RS17500 (BKK81_17500)	coaE	3952305..3952937 (+)	633	WP_071015570.1	dephospho-CoA kinase	-
BKK81_RS17505 (BKK81_17505)	zapD	3953145..3953903 (+)	759	WP_071016592.1	cell division protein ZapD	-
BKK81_RS17510 (BKK81_17510)	-	3953988..3954179 (+)	192	WP_071015571.1	DNA gyrase inhibitor YacG	-
BKK81_RS17515 (BKK81_17515)	-	3954220..3954648 (-)	429	WP_071015573.1	NUDIX domain-containing protein	-
BKK81_RS17520 (BKK81_17520)	-	3954648..3955538 (-)	891	WP_071015574.1	ATP-binding protein	-
BKK81_RS17525 (BKK81_17525)	argJ	3955575..3956801 (-)	1227	WP_071015576.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31657.61 Da
• Isoelectric Point: 7.8053

>NTDB_id=202527 BKK81_RS17495 WP_071015568.1 3951357..3952250(+) (pilD) [Cupriavidus sp. USMAHM13 isolate pure]
MVSAFSPQGAFTIATLAALPGWFLMLAAGLLGLAVGSFLNVVIHRLPRMMEHDEANYIAELRGEPLPHPSRYNLAVPGSA
CPHCGHTLAPWENIPVLSYLLLRGRCSACHAPIGARYPLVELASGVLSVLAAWHFGPTWQGLAALVLVWTLLALTMIDAD
TQLLPDQLTQPLLWLGLLLNLAGVFASLADAVIGAAAGYLLLWSVYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPA
LVLLSSVVGLLFGLVRIALRRQHRDTPFPFGPFIAGAGLLVLFLGAGALPLALGVPG

Nucleotide

• Length: 894 bp

>NTDB_id=202527 BKK81_RS17495 WP_071015568.1 3951357..3952250(+) (pilD) [Cupriavidus sp. USMAHM13 isolate pure]
ATGGTGTCCGCATTCTCGCCGCAAGGCGCCTTCACCATCGCCACGCTGGCCGCCCTGCCCGGCTGGTTCCTGATGCTGGC
GGCCGGCCTGCTGGGCCTGGCGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATGGAGCACGACG
AGGCGAACTACATCGCCGAGCTGCGCGGCGAGCCGCTGCCCCATCCGTCGCGCTACAACCTGGCGGTGCCGGGATCGGCA
TGCCCGCACTGCGGGCACACGCTGGCGCCATGGGAGAACATCCCCGTGCTCAGCTACCTGCTGCTGCGGGGGCGCTGTTC
GGCCTGCCACGCACCGATCGGGGCGCGCTATCCGCTGGTGGAACTCGCCAGCGGGGTGCTGAGCGTGCTGGCCGCCTGGC
ACTTCGGGCCGACCTGGCAGGGCCTGGCGGCGCTGGTCCTGGTGTGGACGCTGCTGGCGCTGACCATGATCGACGCCGAT
ACCCAGCTGCTGCCCGACCAGCTGACCCAGCCGCTGCTGTGGCTCGGCCTGCTGCTCAACCTGGCGGGCGTGTTCGCCTC
GCTGGCCGACGCCGTGATCGGCGCGGCAGCCGGCTACCTGCTGCTGTGGAGCGTCTACTGGCTCTTCAAGCTGGTGCGCG
GCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTGATGGCGGCGCTCGGTGCCTGGTTCGGCTGGCAGGCGCTGCCCGCG
CTGGTGCTGCTGTCCTCCGTGGTCGGCCTGCTGTTCGGCCTCGTCCGCATCGCACTGCGCCGGCAGCATCGCGACACGCC
CTTCCCCTTCGGTCCGTTCATCGCCGGAGCGGGCCTGCTGGTACTGTTCCTGGGGGCCGGCGCGCTGCCGCTCGCGCTGG
GCGTGCCGGGCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (145-254)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (30-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.307	89.899	0.488
pilD	Vibrio campbellii strain DS40M4	53.933	89.899	0.485
pilD	Acinetobacter baumannii D1279779	46.69	96.633	0.451
pilD	Acinetobacter nosocomialis M2	46.341	96.633	0.448
pilD	Neisseria gonorrhoeae MS11	49.027	86.532	0.424